Identification of phytotoxins from Botryosphaeria obtusa, a pathogen of black dead arm disease of grapevine

Djoukeng, Jules Désiré; Polli, Suzanna; Larignon, Philippe; Abou-Mansour, Eliane

doi:10.1007/s10658-008-9419-6

Cited by 70 publications

(98 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Compounds (8) to (10), namely (R)-(À)-mellein, (3R,4R)-4-hydroxymellein and (3R,4S)-4-hydroxymellein, presented [a] 25 D , CD, UV, 1 H and 13 C NMR and ESIMS spectra that were identical to those previously reported (Djoukeng et al, 2009;Cole and Cox, 1981;Asha et al, 2004). The molecular formula of compound (11) .5) were in favour of the hydroxyl substitution C-3.…”

Section: Extraction Purification and Identification Of Phytotoxinssupporting

confidence: 81%

“…All of the compounds tested induced necrosis on host plant leaves of Chardonnay, Pinot noir, and Chasselas and non-host plant leaves of Arabidopsis thaliana (data not included). Despite the known phytotoxic activity of (À)-mellein (8) and (3R,4S) and (3R,4R)-4-hydroxymellein (9, 10) (Djoukeng et al, 2009, Evidente et al, 2010, these compounds were included in the assay to compare their activities with that of the newly isolated (À)-R-3-hydroxymellein (11). The results showed that (À)-R-3-hydroxymellein (11) was the most active at a low concentration (100 lg mL À1 ), inducing a necrosis area greater than 50%, followed by (+)-epi-sphaeropsidone (3) with 25% leaf disc necrosis.…”

Section: Phytotoxic Activitymentioning

confidence: 99%

“…Compounds 8, 9 and 10 were isolated from N. parvum (Evidente et al, 2010) with 7-hydroxymellein and 4,7-dihydroxymellein, which are phytotoxins produced by (D. seriata) B. obtusa from grapevine (Djoukeng et al, 2009). Recently, (À)-mellein (500 lg mL À1 ) was reported to induce partial necrosis on grapevine cv.…”

Section: Phytotoxic Activitymentioning

confidence: 99%

“…(À)-Mellein (8) has been reported to be produced in vivo by D. seriata when grown on powder of grapevine wood (Djoukeng et al, 2009). Further studies are required to determine the concentration causing the observed symptoms before drawing a conclusion regarding the contribution of the phytotoxins to the symptoms of the disease.…”

Section: Analysis Of the Wood Of Grapevine Plants Showing Botryosphaementioning

confidence: 99%

See 3 more Smart Citations

Phytotoxic metabolites from Neofusicoccum parvum, a pathogen of Botryosphaeria dieback of grapevine

et al. 2015

Self Cite

View full text Add to dashboard Cite

Liquid chromatography-diode array screening of the organic extract of the cultures of 13 isolates of the fungus Neofusicoccum parvum, the main causal agent of botryosphaeria dieback of grapevine, showed similar metabolites. One strain was selected for further chemical studies and led to the isolation and characterisation of 13 metabolites. Structures were elucidated through spectroscopic analyses, including one-and two-dimensional NMR and mass spectrometry, and through comparison to literature data. The isolated compounds belong to four different chemical families: five metabolites, namely, (À)-terremutin (1), (+)-terremutin hydrate (2), (+)-epi-sphaeropsidone (3) (À)-4-chloro-terremutin hydrate (4) and(+)-4-hydroxysuccinate-terremutin hydrate (5), belong to the family of dihydrotoluquinones; two metabolites, namely, (6S,7R) asperlin (6) (11), belong to the family of dihydroisocoumarins; and two of the metabolites, namely, 6-methyl-salicylic acid (12) and 2-hydroxypropyl salicylic acid (13), belong to the family of hydroxybenzoic acids. We determined the phytotoxic activity of the isolated metabolites through a leaf disc assay and the expression of defence-related genes in Vitis vinifera cells cv. Chardonnay cultured with (À)-terremutin (1), the most abundant metabolite. Finally, analysis of the brown stripes of grapevine wood from plants showing botryosphaeria dieback symptoms revealed the presence of two of the isolated phytotoxins. and (6R,7S)-dia-asperlin (7), belong to the family of epoxylactones; four metabolites, namely, (R)-(À)-mellein (8), (3R,4R)-4-hydroxymellein (9), (3R,4S)-4-hydroxymellein (10) (R)(À)-3-hydroxymellein

show abstract

Section: Extraction Purification and Identification Of Phytotoxinssupporting

confidence: 81%

Section: Phytotoxic Activitymentioning

confidence: 99%

Section: Phytotoxic Activitymentioning

confidence: 99%

Section: Analysis Of the Wood Of Grapevine Plants Showing Botryosphaementioning

confidence: 99%

See 2 more Smart Citations

Phytotoxic metabolites from Neofusicoccum parvum, a pathogen of Botryosphaeria dieback of grapevine

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…Toxin production by fungi could explain the phenomenon of the appearance of necrosis. Indeed, Djoukeng et al (2009) confirmed the production by Botryosphaeria isolates of toxins that are transported to the herbaceous parts and cause symptoms away from the point of infection. To our knowledge, this is the first report on the genus Botryosphaeria being isolated from grapes in Algeria, and we have also demonstrated the potential pathogenicity of 3 isolates in local grape cultivars.…”

Section: Discussionmentioning

confidence: 64%

Occurrence of Botryosphaeriaceae species associated with grapevine dieback in Algeria

Ammad¹,

Benchabane²,

Toumi³

et al. 2014

Turk J Agric For

View full text Add to dashboard Cite

Grapevine dieback is an increasing problem in grape vineyards in Algeria. We conducted field surveys each spring between 2006 and 2012 to determine the fungal pathogens associated with grapevine decline in various grape-growing regions. We isolated and identified 3 species of Botryosphaeriaceae (Botryosphaeria dothidea, Diplodia seriata, and Neofusicoccum parvum) from infected wood using a combination of morphological and cultural characters, as well as phylogenetic analyses of ribosomal DNA internal transcribed spacer (ITS) regions and fruiting body. We identified 20 isolates of Diplodia seriata, 13 isolates of Botryosphaeria dothidea, and 7 isolates of Neofusicoccum parvum. Inoculation of grapevine plantlets with the 3 Botryosphaeriaceae species produced necrosis and vascular lesions in the wood after a 6-week incubation; Neofusicoccum parvum isolates were the most virulent, followed by D. seriata and B. dothidea. All 3 species were re-isolated from lesions on infected plantlets. Our results represent the first report of a canker disease of grapevine associated with species of the Botryosphaeriaceae in Algeria.

show abstract

Fraxitoxin, a New Isochromanone Isolated from Diplodia fraxini

Cimmino

Maddau

Masi

et al. 2017

Chemistry & Biodiversity

View full text Add to dashboard Cite

A new isochromanone, named fraxitoxin, was isolated together with (-)-mellein and tyrosol from liquid cultures of Diplodia fraxini, a pathogen involved in the etiology of canker and dieback disease of Fraxinus spp. in Europe. It was characterized as 5-methoxy-3-methylisochroman-1-one using spectroscopic methods (essentially NMR and HR-EI-MS). Its absolute configuration (R) at C(3) was assigned by electronic circular dichroism (ECD) measurements and calculations. Phytotoxic activity of the compound was evaluated on ash, cork and holm oak leaves at concentration of 1 mg/ml by the leaf-puncture assay. Interestingly, fraxitoxin caused necrotic lesions only on ash leaves.

show abstract

Identification of phytotoxins from Botryosphaeria obtusa, a pathogen of black dead arm disease of grapevine

Cited by 70 publications

References 14 publications

Phytotoxic metabolites from Neofusicoccum parvum, a pathogen of Botryosphaeria dieback of grapevine

Phytotoxic metabolites from Neofusicoccum parvum, a pathogen of Botryosphaeria dieback of grapevine

Occurrence of Botryosphaeriaceae species associated with grapevine dieback in Algeria

Fraxitoxin, a New Isochromanone Isolated from Diplodia fraxini

Contact Info

Product

Resources

About