Identification of pigmented <i>Serratia marcescens</i> symbiotically associated with <i>Rhynchophorus ferrugineus</i> Olivier (Coleoptera: Curculionidae)

Scrascia, Maria; Pazzani, Carlo; Valentini, F.; Oliva, Marta; Russo, Valentina; D’Addabbo, Pietro; Porcelli, Francesco

doi:10.1002/mbo3.377

Cited by 17 publications

(21 citation statements)

References 34 publications

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“…Some strains have a strong entomopathogenic effect through the production of chitinases and proteinases, for instance for weevils or Drosophila [83,84]. Other strains improve host nutritional status by producing amino acids, or defend their host by enhancing host immunity [85,86]. Both functions enable their hosts to live under challenging conditions.…”

Section: Effects Of Diet On Differences In Gut Microbial Community Comentioning

confidence: 99%

Functional Variation in Dipteran Gut Bacterial Communities in Relation to Their Diet, Life Cycle Stage and Habitat

Sontowski

Dam

2020

Insects

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True flies and mosquitos (Diptera) live in habitats and consume diets that pose specific demands on their gut bacterial communities (GBCs). Due to diet specializations, dipterans may have highly diverse and species-specific GBCs. Dipterans are also confronted with changes in habitat and food sources over their lifetime, especially during life history processes (molting, metamorphosis). This may prevent the development of a constant species- or diet-specific GBC. Some dipterans are vectors of several human pathogens (e.g., malaria), which interact with GBCs. In this review, we explore the dynamics that shape GBC composition in some Diptera species on the basis of published datasets of GBCs. We thereby focus on the effects of diet, habitats, and life cycle stages as sources of variation in GBC composition. The GBCs reported were more stage-specific than species- or diet-specific. Even though the presence of GBCs has a large impact on the performance of their hosts, the exact functions of GBCs and their interactions with other organisms are still largely unknown, mainly due to the low number of studies to date. Increasing our knowledge on dipteran GBCs will help to design pest management strategies for the reduction of insecticide resistance, as well as for human pathogen control.

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Section: Effects Of Diet On Differences In Gut Microbial Community Comentioning

confidence: 99%

Functional Variation in Dipteran Gut Bacterial Communities in Relation to Their Diet, Life Cycle Stage and Habitat

Sontowski

Dam

2020

Insects

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“…Serratia marcescens is present as part of the gut microbiota of some sandflies and triatomine species [ 11 ]. Examples of S. marcescens , isolated as a pathogen from insects of the orders Coleoptera, Diptera, Orthoptera, and Lepidoptera, have been reported [ 12 , 13 , 14 , 15 , 16 , 17 , 18 ]. Similarly, P. aeruginosa has been isolated from different weevil species (the pecan weevil Curculio caryae [ 14 ]; the southern pine weevil Dendroctonus brevicomis [ 12 ]; the red palm weevil Rynchophorus ferrugineus [ 19 ], the tobacco and tomato hornworms Manduca sexta and M. quinquemaculata [ 20 ], from Schistocerca gregaria eggs [ 21 ] in the wild and from several other insect species.…”

Section: Introductionmentioning

confidence: 99%

Cannibalism as a Possible Entry Route for Opportunistic Pathogenic Bacteria to Insect Hosts, Exemplified by Pseudomonas aeruginosa, a Pathogen of the Giant Mealworm Zophobas morio

Maciel-Vergara

Jensen

Eilenberg

2018

Insects

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Opportunistic bacteria are often ubiquitous and do not trigger disease in insects unless the conditions are specifically favorable for bacterial development in a suitable host. In this paper, we isolated and identified a bacterium, Pseudomonas aeruginosa, from the larvae of the giant mealworm Zophobas morio and we studied the possible entry routes by challenging larvae with per os injection and subdermal injection. We also evaluated the effect of exposing groups of larvae to P. aeruginosa inoculated in their feed and the effect of exposing wounded larvae to P. aeruginosa. We concluded that the mortality rate of Z. morio larvae is higher when P. aeruginosa gets in direct contact with the hemolymph via intracoelomic injection compared to a situation where the bacterium is force-fed. Larvae with an open wound exposed to P. aeruginosa presented higher mortality rate compared to larvae with a wound that was not exposed to the bacterium. We documented too, that cannibalism and scavenging were more prevalent among larvae in a group, when P. aeruginosa is present compared to when it is absent. We discuss hereby different aspects related with the pathogen’s entry routes to insects the complexity of pathogen´s transmission in high population densities and different ways to prevent and/or control P. aeruginosa in mass rearing systems.

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“…S. marcescens was regularly isolated from the reproductive apparatus of adult RPWs, deposited eggs, and along the tissue of infested palms. Isolation was performed in Southern Italy from 2009 to 2014 [20]. Based on different places and years of isolation (Table 1), 4 strains (S1, S5, S8, and S13), collected from the reproductive apparatus of RPWs were randomly selected for whole genome sequencing and detection of CRISPR-Cas systems.…”

Section: Methodsmentioning

confidence: 99%

Characterization of CRISPR-Cas Systems in Serratia marcescens Isolated from Rhynchophorus ferrugineus (Olivier, 1790) (Coleoptera: Curculionidae)

et al. 2019

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The CRISPR-Cas adaptive immune system has been attracting increasing scientific interest for biological functions and biotechnological applications. Data on the Serratia marcescens system are scarce. Here, we report a comprehensive characterisation of CRISPR-Cas systems identified in S. marcescens strains isolated as secondary symbionts of Rhynchophorus ferrugineus, also known as Red Palm Weevil (RPW), one of the most invasive pests of major cultivated palms. Whole genome sequencing was performed on four strains (S1, S5, S8, and S13), which were isolated from the reproductive apparatus of RPWs. Subtypes I-F and I-E were harboured by S5 and S8, respectively. No CRISPR-Cas system was detected in S1 or S13. Two CRISPR arrays (4 and 51 spacers) were detected in S5 and three arrays (11, 31, and 30 spacers) were detected in S8. The CRISPR-Cas systems were located in the genomic region spanning from ybhR to phnP, as if this were the only region where CRISPR-Cas loci were acquired. This was confirmed by analyzing the S. marcescens complete genomes available in the NCBI database. This region defines a genomic hotspot for horizontally acquired genes and/or CRISPR-Cas systems. This study also supplies the first identification of subtype I-E in S. marcescens.

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Identification of pigmented Serratia marcescens symbiotically associated with Rhynchophorus ferrugineus Olivier (Coleoptera: Curculionidae)

Cited by 17 publications

References 34 publications

Functional Variation in Dipteran Gut Bacterial Communities in Relation to Their Diet, Life Cycle Stage and Habitat

Functional Variation in Dipteran Gut Bacterial Communities in Relation to Their Diet, Life Cycle Stage and Habitat

Cannibalism as a Possible Entry Route for Opportunistic Pathogenic Bacteria to Insect Hosts, Exemplified by Pseudomonas aeruginosa, a Pathogen of the Giant Mealworm Zophobas morio

Characterization of CRISPR-Cas Systems in Serratia marcescens Isolated from Rhynchophorus ferrugineus (Olivier, 1790) (Coleoptera: Curculionidae)

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