Identification of piroplasm infection in questing ticks by RLB: a broad range extension of tick-borne piroplasm in China?

Abdallah, Mirza Omar; Niu, Qingli; Yu, Peifa; Guan, Guiquan; Yang, Jifei; Chen, Ze; Liu, Guangyuan; YongHong, Wei; Luo, Jianxun; Yin, Hong

doi:10.1007/s00436-016-4947-z

Cited by 9 publications

(5 citation statements)

References 50 publications

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“…The protection rate in the vaccinated groups was 100% and it is closely similar to the finding recorded in China which ranged from 99.5 to 99.9% [29][30][31][32][33][34][35] . Coinfection with other tick-borne pathogens was detected through the RLB assay which is more sensitive when compared to blood smears, PCR, and nested PCR, suggesting that all co-infection occurred at low levels 2,36 . The higher co-infections in the unvaccinated (control) group compared to the vaccinated group could be attributed to the heterologous or non-specific immunological effects (NSE) that can be induced when using live attenuated vaccines.…”

Section: Discussionmentioning

confidence: 99%

Isolation and propagation of an Egyptian Theileria annulata infected cell line and evaluation of its use as a vaccine to protect cattle against field challenge

AL-Hosary,

Radwan,

Ahmed

et al. 2024

Sci Rep

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Tropical theileriosis is an important protozoan tick-borne disease in cattle. Vaccination using attenuated schizont-infected cell lines is one of the methods used for controlling the disease. This study describes the production of attenuated schizont-infected cell lines from Egypt and an evaluation of its use as a vaccine to protect calves against clinical disease upon field challenge. Two groups of exotic and crossbred male calves were divided into vaccinated and control groups. The vaccinated groups were inoculated with 4 ml (1 × 106 cells/ml) of the attenuated cell line. Three weeks after vaccination, calves of both groups were transported to the New Valley Governorate (Egyptian oasis) where they were kept under field conditions and exposed to the natural Theileria annulata challenge. All animals in the control group showed severe clinical signs and died despite treatment with buparvaquone, which was administered after two days of persistent fever due to a severe drop in packed cell volume (PCV). Animals in the vaccinated group became seropositive without developing severe clinical signs other than transient fever. Post-mortem examinations revealed enlarged and fragile lymph nodes, spleen, and liver with necrosis and hemorrhages. These findings indicate that the Egyptian attenuated cell line was successful in protecting both exotic and crossbred animals against tropical theileriosis under field conditions.

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Section: Discussionmentioning

confidence: 99%

Isolation and propagation of an Egyptian Theileria annulata infected cell line and evaluation of its use as a vaccine to protect cattle against field challenge

AL-Hosary,

Radwan,

Ahmed

et al. 2024

Sci Rep

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show abstract

“…The protection rate in the vaccinated groups was 100% and it is closely similar to the nding recorded in China which ranged from 99.5-99.9% [27][28][29][30][31][32][33] . Co-infection with other tick-borne pathogens was detected through the RLB assay which is more sensitive when compared to blood smears, PCR, and nested PCR, suggesting that all co-infection occurred at low levels 2,34 . The higher co-infections in nonvaccinated (Control) group when compared with the vaccinated one could be attributed to the heterologous or non-speci c immunological effects (NSE) that may be induced when live-attenuated vaccines are used.…”

Section: Discussionmentioning

confidence: 99%

Isolation and propagation of an Egyptian Theileria annulata infected cell line and evaluation of its use as a vaccine to protect cattle against field challenge

AL-Hosary

Radwan²,

Ahmed

et al. 2023

Preprint

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Tropical theileriosis is an important protozoan tick-borne disease of cattle. Vaccination using attenuated schizont cell lines is one of the methods used for controlling the disease. This study describes the production of an attenuated T. annulata cell line from Egypt and an evaluation of its use as a vaccine to protect calves against clinical disease upon field challenge. Two groups of exotic and crossbred male calves were divided into vaccinated and control groups. Vaccinated groups were inoculated with 4 ml (1 x 106 cells/ml) of the attenuated cell line. Three weeks after vaccination, calves of both groups were transported to the New Valley governorate where they were kept under field conditions and exposed to natural T. annulata challenge. All animals of the control group showed severe clinical signs and died despite treatment with buparvaquone after a severe drop in the packed cell volume (PCV). Animals of the vaccinated group became seropositive without developing serious clinical signs except for the transient fever. Post-mortem examinations revealed enlarged and fragile lymph nodes, spleen, and liver with necrosis and hemorrhages. These findings indicate that the Egyptian attenuated cell line was successful in protecting both exotic and crossbred animals against tropical theileriosis under field conditions.

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“…In such a scenario, utilization of generic primers poses the problem of correct allocation to the respective species or species complex. DNA sequencing without prior cloning of the less prevalent amplicons is often unsuccessful or distorts the whole readout making it at times incomprehensible [21]. Furthermore, the pathogen concentration in the host blood varies dramatically depending on the animal’s state of infection, making the identification challenging when present in very low concentrations.…”

Section: Discussionmentioning

confidence: 99%

Development of a Low-Density DNA Microarray for Detecting Tick-Borne Bacterial and Piroplasmid Pathogens in African Cattle

Abanda

Paguem

Achukwi³

et al. 2019

TropicalMed

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In Africa, pathogens transmitted by ticks are of major concern in livestock production and human health. Despite noticeable improvements particularly of molecular screening methods, their widespread availability and the detection of multiple infections remain challenging. Hence, we developed a universally accessible and robust tool for the detection of bacterial pathogens and piroplasmid parasites of cattle. A low-cost and low-density chip DNA microarray kit (LCD-Array) was designed and tested towards its specificity and sensitivity for five genera causing tick-borne diseases. The blood samples used for this study were collected from cattle in Northern Cameroon. Altogether, 12 species of the genera Anaplasma, Ehrlichia, Rickettsia and Theileria, and their corresponding genus-wide probes including Babesia were tested on a single LCD-Array. The detection limit of plasmid controls by PCR ranged from 1 to 75 copies per µL depending on the species. All sequenced species hybridized on the LCD-Array. As expected, PCR, agarose gel electrophoresis and Sanger sequencing found significantly less pathogens than the LCD-Array (p < 0.001). Theileria and Rickettsia had lower detection limits than Anaplasma and Ehrlichia. The parallel identification of some of the most detrimental tick-borne pathogens of livestock, and the possible implementation in small molecular-diagnostic laboratories with limited capacities makes the LCD-Array an appealing asset.

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Identification of piroplasm infection in questing ticks by RLB: a broad range extension of tick-borne piroplasm in China?

Cited by 9 publications

References 50 publications

Isolation and propagation of an Egyptian Theileria annulata infected cell line and evaluation of its use as a vaccine to protect cattle against field challenge

Isolation and propagation of an Egyptian Theileria annulata infected cell line and evaluation of its use as a vaccine to protect cattle against field challenge

Isolation and propagation of an Egyptian Theileria annulata infected cell line and evaluation of its use as a vaccine to protect cattle against field challenge

Development of a Low-Density DNA Microarray for Detecting Tick-Borne Bacterial and Piroplasmid Pathogens in African Cattle

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