Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey

Abstract

Purpose of this study was to examine leptin (LEP) and pituitary-specific transcription factor-1 (Pit-I) gene polymorphisms in Holstein cattle (n=352) in Turkey. In order to determine the Pit-I-HinfI and LEP-Sau3AI polymorphisms, polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were performed. A 600 bp length fragment of Pit-I and a 422 bp length fragment of LEP were amplified. In this study, two types of alleles, A and B, for both of the Pit-I and LEP genes were observed. The highest frequencies of the alleles were estimated, and were the B allele (0.68) for the Pit-I gene and the A allele (0.87) for the LEP gene. According to the results of the chi-square test, a significant deviation from the Hardy-Weinberg equilibrium was determined for both of these genes in the investigated breed (in the same order; X 2 :40.75, p<0.001and X 2 :8.4, p<0.01). Türkiye'de yetiştirilen Holştayn sığırlarda hipofiz bezine özgü transkripsiyon faktörü-1 (PIT-1) ve leptin (LEP) gen polimorfizmlerinin belirlenmesiÖzet: Bu çalışmada Türkiye'de Kayseri civarında yetiştirilen Holştayn ırkı sığırlarda (n=352) leptin (LEP) ve hipofiz bezine özgü transkripsiyon faktörü -1 (Pit-I) genlerinin polimorfizmlerinin belirlenmesi amaçlanmıştır. Pit-I-HinfI ve LEP-Sau3AI polimorfizmlerinin belirlenmesi amacı ile polimeraz zincir reaksiyonu (PZR) ve restriksiyon parça uzunluk polimorfizm (RFLP) analizi yapılmıştır. Pit-1 geni için 600 bç'lik ve LEP geni için 422 bç'lik uzunluk amplifiye edilmiştir. Çalışmada, Pit-1 ve LEP geni allellerinin her ikisinde de A ve B allelleri olmak üzere iki iki tip allel gözlendi. En yüksek frekans Pit-1 geni için A allelinde (0.87), LEP geni için ise B allelinde (0.68) olarak belirlendi. Ki-kare test sonuçlarına gore, incelenen ırkta, her iki gende de Hardy-Weinberg dengesinden anlamlı bir sapma gözlendi (aynı sıra ile; X 2 :40.75, p<0.001ve X 2 :8.4, p<0.01).

Association between GH (g.1456_1457insT), GHRH (g.4474 C>A), and Pit-1 (g.244G>A) Polymorphisms and Lactation Traits in Holstein Friesian Cattle

“…Genes controlling lactation traits in dairy animals are of great concern during breeding programs due to their decisive economic values. Growth genes could be functioned as markers assisted selection (MAS) due to their main roles on growth, development of udder cells, process of lactogenesis, and proliferation of udder cells, and lactation traits (Ardicli et al, 2019;Bayram et al, 2017;Sami et al, 2011;Szatkowska et al, 2009). This study examined possible influences of the GH (g.1456_1457 InsT), GHRH (g.4474 C>A), and Pit-1 (g.244 G>A) genotypes on milk yield and milk quality in Holstein Friesian cattle.…”

“…Differences in lactation months and lactation periods were considered in the GLM analysis, but any non genetic factors had other significant effects. Further, milk production and lactation traits are quantitative traits controlled by many alleles and many genes (Ahmadi et al, 2015;Ahmadzadeh et al, 2019;Bayram et al, 2017;Raschia et al, 2018). Therefore, the possible differences in allele frequencies of the GH g.1456_1457 InsT, GHRH g.4474 C>A SNP, and Pit-1 g.244 G>A SNP in one or more populations as well as the influences of many other genes could be the cause of no association of the variant genotypes of one or more of these loci on milk production and milk quality.…”

“…Selection techniques at the DNA level and quantitative trait locus (QTL) can help to accelerate quantitative selections through the identification of major genes controlling the economic traits. Milk yield and its quality in dairy cattle are quantitative traits and controlled by many genes which are potentially influenced by the growth genes (Ahmadi et al, 2015;Ahmadzadeh et al, 2019;Bayram et al, 2017;Szatkowska et al, 2009).…”

“…The growth genes providing GH, GHRH, and Pit1 genes influence biological functions of the livestock, such as growth, development, proliferation of udder cells, and lactogenesis (Ahmadi et al, 2015;Ahmadzadeh et al, 2019;Bayram et al, 2017;Kiyici et al, 2019;Sami et al, 2011;Szatkowska et al, 2009). Bovine Growth Hormone Releasing Hormone (bGHRH) gene consists of five exons and four introns and locates in the chromosome number 13 (BTA-13).…”