1975
DOI: 10.1104/pp.55.3.550
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Identification of Plant Hormones from Cotton Ovules

Abstract: An extract from 8-day-old cotton ovules (Gossypiumii hirsutuint L.) was partitioned into three fractions and each fraction was derivatized and analyzed separately. Gas-liquid chromatography and computer-controlled gas-liquid chromatography-mass spectrometry were used to separate, measure, and identify the naturally occurring plant hormones. A single extract contained abscisic acid, indoleacetic acid, and gibberellins A1, A3, A4. A7, A9, and A13 in the first fraction; ethyl indole-3-acetate and indole-3-aldehyd… Show more

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Cited by 162 publications
(85 citation statements)
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“…For estimation of growth regulators, 3-5 g fresh samples of the youngest fully expanded leaf were frozen in liquid nitrogen and stored at -80°C until analysis. The method of extraction in ethanol and the fraction of the ethanol extract were carried out according to the method described by Shindy and Smith (1975). The acidic fraction contained the acidic hormones (IAA, GA 3 and ABA) while the aqueous fraction comprised the cytokinin.…”
Section: Assay Of Plant Hormonesmentioning
confidence: 99%
“…For estimation of growth regulators, 3-5 g fresh samples of the youngest fully expanded leaf were frozen in liquid nitrogen and stored at -80°C until analysis. The method of extraction in ethanol and the fraction of the ethanol extract were carried out according to the method described by Shindy and Smith (1975). The acidic fraction contained the acidic hormones (IAA, GA 3 and ABA) while the aqueous fraction comprised the cytokinin.…”
Section: Assay Of Plant Hormonesmentioning
confidence: 99%
“…The fraction of the ethanol extract was carried out according to the method described by Wasfy et al (1974). The Acidic fraction contain the acidic hormones (IAA, GA, and ABA) while the aqueous fraction comprised the cytokinins.…”
Section: Hormonal Analysismentioning
confidence: 99%
“…Endogenous ABA level was estimated using Gas-Liquid Chromatography according to Shindy & Smith (1975).…”
Section: Estimation Of Endogenous Aba Levelsmentioning
confidence: 99%