Identification of plant volatiles activating single receptor neurons in the pine weevil ( Hylobius abietis )

Wibe, Atle; Borg‐Karlson, Anna‐Karin; Norin, Torbjörn; Mustaparta, Hanna

doi:10.1007/s003590050075

Cited by 47 publications

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“…Several species related to A. musculus, e.g., Anthonomis pomorum, A. grandis, and A. rubi, have shown electrophysiological antennal (EAG) responses to host plant volatiles (Dickens, 1990;Kalinova et al, 2000;Bichao et al, 2005) and several species of weevils, such as the pine weevil, banana weevil, vine weevil, and pepper weevil (Budenberg et al, 1993;Wibe et al, 1997;van Tol and Visser, 2002;Addesso and McAuslane, 2009), responded to host-plant volatiles in behavioral bioassays. In Y-tube olfactometer assays, Mechaber (1992) found that adult A. musculus were more attracted to conspecific-damaged cranberry vines compared with undamaged vines, as well as to undamaged and damaged flower buds compared to clean air (Mechaber, 1992).…”

Section: Resultsmentioning

confidence: 99%

Identification and Field Evaluation of Attractants for the Cranberry Weevil, Anthonomus musculus Say

et al. 2011

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Studies were conducted to develop an attractant for the cranberry weevil, Anthonomus musculus, a pest of blueberry and cranberry flower buds and flowers in the northeastern United States. In previous studies, we showed that cinnamyl alcohol, the most abundant blueberry floral volatile, and the green leaf volatiles (Z)-3-hexenyl acetate and hexyl acetate, emitted from both flowers and flower buds, elicit strong antennal responses from A. musculus. Here, we found that cinnamyl alcohol did not increase capture of A. musculus adults on yellow sticky traps compared with unbaited controls; however, weevils were highly attracted to traps baited with the Anthonomus eugenii Cano aggregation pheromone, indicating that these congeners share common pheromone components. To identify the A. musculus aggregation pheromone, headspace volatiles were collected from adults feeding on blueberry or cranberry flower buds and analyzed by gas chromatographymass spectrometry. Three male-specific compounds were identified: (Z)-2-(3,3-dimethyl-cyclohexylidene) ethanol (Z grandlure II); (Z)-(3,3-dimethylcyclohexylidene) acetaldehyde (grandlure III); and (E)-(3,3-dimethylcyclohexylidene) acetaldehyde (grandlure IV). A fourth component, (E)-3,7-dimethyl-2,6-octadien-1-ol (geraniol), was emitted in similar quantities by males and females. The emission rates of these volatiles were about 2.8, 1.8, 1.3, and 0.9 ng/adult/d, respectively. Field experiments in highbush blueberry (New Jersey) and cranberry (Massachusetts) examined the attraction of A. musculus to traps baited with the male-produced compounds and geraniol presented alone and combined with (Z)-3-hexenyl acetate and hexyl acetate, and to traps baited with the pheromones of A. eugenii and A. grandis. In both states and crops, traps baited with the A. musculus maleproduced compounds attracted the highest number of adults. Addition of the green leaf volatiles did not affect A. musculus attraction to its pheromone but skewed the sex ratio of the captured adults towards females. Although the role of plant volatiles in host-plant location by A. musculus is still unclear, our studies provide the first identification of the primary A. musculus aggregation pheromone components that can be used to monitor this pest in blueberry and cranberry pest management programs.

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Section: Resultsmentioning

confidence: 99%

Identification and Field Evaluation of Attractants for the Cranberry Weevil, Anthonomus musculus Say

et al. 2011

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“…Some of the plant compounds presented here as relevant stimuli for RN types I and IV, have in previous studies been shown to elicit electroantennograms (EAG) in heliothine moths (Park et al 2002;Bruce & Cork 2001;Burguiere et al 2001), and to elicit EAG or activate single RNs in other lepidopteran, coleopteran and hemipteran species (Bäckman et al 2000;Jönsson & Anderson 1999;Bichão et al 2003;Barata et al 2000;Wibe et al 1997;Dickens 1990;Weissbecker et al 2000). Particularly interesting here is the finding in Spodoptera littoralis (Boisduval) of a RN type responding strongly to α-farnesene and E,E-TMTT and weaker responses to DMNT and geraniol (Jönsson & Anderson 1999).…”

Section: Discussionmentioning

confidence: 99%

Receptor neurones in three heliothine moths responding to floral nd inducible plant volatiles

et al. 2003

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Some plant volatiles are produced in response to herbivory of several insect species, including heliothine larvae. In the present study of female heliothine moths, four co-located receptor neurone types were identified, of which three types responded strongest to the inducible compounds E-β-ocimene, E,E-α-farnesene and E,E-TMTT, respectively. The fourth type responded strongest to geraniol, which is a common floral volatile. The narrow tuning of each receptor neurone type was demonstrated by responses to a few structurally-related monoterpenes, sesquiterpenes, homo-terpenes and monoterpene alcohols, respectively, out of hundreds of plant constituents tested. The four neurone types showed the same relation of spike amplitudes and ranking of effective compounds in the three heliothine species; the polyphagous Heliothis virescens and Helicoverpa armigera and the oligophagous Helicoverpa assulta. The results indicate the presence of functionally similar types of plant odour-receptor neurones in the three related species, and suggest conservation or reappearance of functionally similar olfactory receptors in related species, independent of the evolution of polyphagy and oligophagy.

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“…Also the pink bollworm moth, Pectinophora gossypiella, one of the major pests on the β-caryophyllene rich cotton plants, has specific ORNs with high sensitivty to β-caryophyllene (Tömmerdal, 1995). In other studies, the use of gas chromatography linked to electrophysiology has been attempted to test single ORNs in oligophagous and polyphagous species for, in principle, all of the naturally produced constituents in the relevant plants (Blight et al, 1995;Wibe & Mustaparta, 1996;Wibe et al, 1997;Rostelien et al, 2000a,b;Stranden et al, 2002) Stensmyr et al, 2001Barata et al, 2002). Interestingly, in two beetle species (Hylobius abietis and Phoracanta semipunctata) considered as oligophagous, many different ORN types have so far been identified that respond to compounds common in many plants.…”

Section: Orns In Oligophagous Versus Polyphagous Speciesmentioning

confidence: 99%

Encoding of plant odour information in insects: peripheral and central mechanisms

Mustaparta

2002

Entomologia Exp Applicata

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Insects are suitable model organisms for studying mechanisms underlying olfactory coding and olfactory learning, by their unique adaptation to host plants in which the chemical senses are essential. Recent molecular biological studies have shown that a large number of genes in insects and other organisms are coding for olfactory receptor proteins. In general, one receptor type seems to be expressed in each neurone. The functional characterisations of olfactory receptor neurones have been extensive in certain insect species, demonstrating a fine-tuning of single neurones to biologically relevant odourants; both insect and plant produced volatiles. Stained neurones of the same functional type have been shown to project in one and the same glomerular unit in the primary olfactory centre, the antennal lobe. This corresponds to molecular biological studies, showing projections in one glomerulus by neurones expressing the same receptor type. Comparison of these findings with physiological and morphological characterisations of antennal lobe neurones has indicated correspondence between input and output of the glomerular units. Examples are presented from studies of heliothine moths. From the antennal lobe, the olfactory information is further conveyed to the mushroom bodies, particularly important for learning, and the lateral protocerebrum, a premotoric area. The three brain areas are regions of synaptic plasticity important in learning of odours, which is well studied in the honeybee but also in species of moths.

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Identification of plant volatiles activating single receptor neurons in the pine weevil ( Hylobius abietis )

Cited by 47 publications

References 0 publications

Identification and Field Evaluation of Attractants for the Cranberry Weevil, Anthonomus musculus Say

Identification and Field Evaluation of Attractants for the Cranberry Weevil, Anthonomus musculus Say

Receptor neurones in three heliothine moths responding to floral nd inducible plant volatiles

Encoding of plant odour information in insects: peripheral and central mechanisms

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