Identification of plasma microRNAs as novel noninvasive biomarkers for early detection of lung cancer

Tang, Dongfang; Shen, Yi; Wang, Mingzhao; Yang, Ronghua; Wang, Zizong; Sui, Aihua; Jiao, Wenjie; Wang, Yongjie

doi:10.1097/cej.0b013e32835f3be9

Cited by 112 publications

(87 citation statements)

References 28 publications

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“…These reports have characterized miR-21, miR-155 and miR-18a (miR-17-92 cluster) as oncogenic miRNAs (oncomirs) [Lu et al, 2011;Lu and Rothenberg, 2013]. The expression of these oncomirs has been associated directly with increased lung cancer severity in humans [Volinia et al, 2006;Tang et al, 2013;Yang et al, 2013]. The activation of specific oncomirs raises a new question: are individuals who were exposed to SHS in utero at heightened risk as adults for certain cancers?…”

Section: Discussionmentioning

confidence: 99%

“…1.2E-03 Asthma Winkler et al, 2014;Wu et al, 2014], Cancer [Seike et al, 2009;Tang et al, 2013;Yang et al, 2013], $: "2.0 #: "2.4* miR-298-5p 1.2E-03 $: "2.0* #: "2.5* miR-341-3p…”

Section: Tumor Suppressor Genes Predicted Targets Of the Activated Omentioning

confidence: 99%

“…With complete transcriptome screening results for miRNA and mRNA, we interrogated the potential target genes of oncomirs, to test whether oncomirs, with their transcriptional suppression capability, act to downregulate tumor suppressor genes in this model and -Nunez et al, 2011;Malmhall et al, 2014], Cancer [Tang et al, 2013;Yang et al, 2013] $: "2.4* #: "2.0* miR-541-5p 2.8E-05 $: #1.3 #: "1.8* miR-511-3p 9.8E-04 Cancer [Zhang et al, 2012] $: "1.3 #: "1.7* miR-21-3p…”

Section: Tumor Suppressor Genes Predicted Targets Of the Activated Omentioning

confidence: 99%

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In utero exposure to second‐hand smoke activates pro‐asthmatic and oncogenic miRNAs in adult asthmatic mice

Xiao

Noël

Perveen

et al. 2016

Environ and Mol Mutagen

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Exposures to environmental pollutants contribute to dysregulated microRNA (miRNA) expression profiles, which have been implicated in various diseases. Previously, we reported aggravated asthmatic responses in ovalbumin (OVA)‐challenged adult mice that had been exposed in utero to second‐hand smoke (SHS). Whether in utero SHS exposure dysregulates miRNA expression patterns in the adult asthma model has not been investigated. Pregnant BALB/c mice were exposed (days 6–19 of pregnancy) to SHS (10 mg/m3) or HEPA‐filtered air. All offspring were sensitized and challenged with OVA (19–23 weeks) before sacrifice. RNA samples extracted from lung homogenates, were subjected to RNA sequencing (RNA‐seq). RNA‐seq identified nine miRNAs that were most significantly up‐regulated by in utero SHS exposure. Among these nine, miR‐155‐5p, miR‐21‐3p, and miR‐18a‐5p were also highly correlated with pro‐asthmatic Th2 cytokine levels in bronchoalveolar lavage fluid. Further analysis indicated that these up‐regulated miRNAs shared common chromosome locations, particularly Chr 11C, with pro‐asthmatic genes. These three miRNAs have also been characterized as oncogenic miRNAs (oncomirs). We cross‐referenced miRNA‐mRNA expression profiles and identified 16 tumor suppressor genes that were down‐regulated in the in utero‐exposed offspring and that are predicted targets of the up‐regulated oncomirs. In conclusion, in utero SHS exposure activates pro‐asthmatic genes and miRNAs, which colocalize at specific chromosome locations, in OVA‐challenged adult mice. The oncogenic characteristics of the miRNAs and putative miRNA‐mRNA regulatory networks suggest that the synergistic effect of in utero SHS exposure and certain adult irritants may promote an oncogenic milieu in mouse lungs via inhibition of miRNA‐regulated tumor suppressor genes. Environ. Mol. Mutagen. 57:190–199, 2016. © 2016 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 99%

“…1.2E-03 Asthma Winkler et al, 2014;Wu et al, 2014], Cancer [Seike et al, 2009;Tang et al, 2013;Yang et al, 2013], $: "2.0 #: "2.4* miR-298-5p 1.2E-03 $: "2.0* #: "2.5* miR-341-3p…”

Section: Tumor Suppressor Genes Predicted Targets Of the Activated Omentioning

confidence: 99%

Section: Tumor Suppressor Genes Predicted Targets Of the Activated Omentioning

confidence: 99%

See 1 more Smart Citation

In utero exposure to second‐hand smoke activates pro‐asthmatic and oncogenic miRNAs in adult asthmatic mice

Xiao

Noël

Perveen

et al. 2016

Environ and Mol Mutagen

View full text Add to dashboard Cite

show abstract

“…A panel of 5 miRNAs, including 3 with increased plasma concentrations (miR-21-5p, -182-5p, and -210-3p) and 2 with decreased concentrations (miR-126-3p and -486-5p) provided good diagnostic power for all stages of NSCLC (43,44 ). Additionally, the increased miR-21-5p and -155-5p and decreased miR-145 concentrations in plasma also showed good diagnostic potential in differentiating patients with NSCLC from healthy smokers (45,46 ). Although it is not included in our analysis, the change in concentration of specific circulating miRNAs has also been reported as a prognostic marker.…”

Section: Lung Cancermentioning

confidence: 97%

Current State of Circulating MicroRNAs as Cancer Biomarkers

et al. 2015

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BACKGROUND:Numerous studies have demonstrated the existence of stable regulatory RNAs, microRNAs (miRNAs), in the circulation and have shown that the spectrum of these extracellular miRNAs is affected by various pathologic conditions including cancers.CONTENT: Circulating miRNAs have been the focus of numerous cancer biomarker discovery efforts over the past few years; however, a considerable number of these studies have yielded inconsistent and irreproducible findings. Here, we have summarized and compared the results of studies covering 8 different cancer types to address key questions, including the possibility of using circulating miRNA to detect cancers and what factors may affect miRNA signatures. Although identifying circulating miRNA signatures to detect specific types of early stage cancers can be challenging, study results suggest that it may be possible to use miRNAs to detect cancers in general.

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“…It has been proven that small noncoding RNAs, including micro-RNAs, shows great stability in the circulation with the state of microRNA-processing proteins (47). The micro-RNAs have oncogenic and tumor suppressor gene function and exhibit specific expression profiles in lung cancer, and thus have been proposed as diagnostic and prognostic markers (48)(49)(50)(51). In particular, the work of Montani et al identified a signature of several plasma microRNA that showed an excellent predictive value for lung cancer in a high-risk population (52).…”

Section: Circulating Nucleic Acidsmentioning

confidence: 99%

Screening for early stage lung cancer and its correlation with lung nodule detection

Qian¹,

Yang²,

Chen³

et al. 2018

J. Thorac. Dis.

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Currently, the most effective way of reducing lung cancer mortality is early diagnosis of lung cancer. The National Lung Screening Trial has proved the efficacy of lung cancer screening using lowdose computed tomography to reduce lung cancer mortality. However, many questions remain surrounding lung cancer screening implementation, among which include how to select the optimal risk population, the personalized screening interval based different levels of risk, methods to improve diagnostic discrimination between malignant and benign disease in detected lung nodules, and the roles of biomolecular markers in stratifying risk and in guiding the management of indeterminate nodules. This review concentrates on the latest developments of lung cancer screening and provides an overview of the main unanswered questions on lung nodule detection. J Thorac Dis 2018;10(Suppl 7):S846-S859 jtd.amegroups.com reviews the latest progress in lung cancer screening and the main unanswered questions on lung nodule detection, to discuss optimal strategies for implementation of lung cancer detection. How to conduct screening Screening by thoracic imageryThe NLST enrolled high-risk asymptomatic individuals: age, 55-74 years; current or former smokers who quit within 15 years with at least 30 pack-years of smoking history. From August 2002 through April 2004 individuals were randomly assigned to annual LDCT screening scans versus CXR for three consecutive years. After a median follow-up of 6.5 years, the study showed that screening lead to a significant reduction of 20% and 6.7% in lung cancer and overall mortality, respectively. LDCT showed better performance for the detection of early-stage lung cancer, with 57% of screening-detected lung cancer cases of stages I or II, compared to only 39% in the CXR arm (9). This has led to the American Cancer Society's guidelines now recommending LDCT screening in high risk individuals aged 55 to 80 years who have a 30-year pack smoking history and currently smoke or have quit within the past 15 years (12).The impact of LDCT screening versus usual care or CXR have been also compared by several large-scale European randomized trials: Dutch-Belgian Randomized Lung Cancer Screening (NELSON) trial (13) (19). So far, the three published trials have reported conflicting results against NLST. The DLCST showed higher rate of all-cause mortality in the CT screening arm compared with usual care [relative risk (RR) 1.03; 95% CI: 0.66-1.60] (20,21). In the DANTE trial, CT screening demonstrated no significant effect on reduce of mortality (RR 0.99; 95% CI: 0.69-1.43) (15). The MILD trial found no effect of biennial screening compared with usual care and a negative effect of annual screening with more detected cancers but also higher all-cause mortality (RR 1.99; 95% CI: 0.80-4.96) (16). It has raised questions about whether the much smaller patient sample size and the inclusion of patients at lower risk (less exposure to tobacco) for lung cancer in these trials diluted any potential benefits of LDCT s...

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Identification of plasma microRNAs as novel noninvasive biomarkers for early detection of lung cancer

Cited by 112 publications

References 28 publications

In utero exposure to second‐hand smoke activates pro‐asthmatic and oncogenic miRNAs in adult asthmatic mice

In utero exposure to second‐hand smoke activates pro‐asthmatic and oncogenic miRNAs in adult asthmatic mice

Current State of Circulating MicroRNAs as Cancer Biomarkers

Screening for early stage lung cancer and its correlation with lung nodule detection

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