Identification of plasmid IncQ1 and NTEKPC-IId harboring bla KPC-2 in isolates from Klebsiella pneumoniae infections in patients from Recife-PE, Brazil

Lima, Giselle Jucá de; Scavuzzi, Alexsandra Maria Lima; Beltrão, Elizabeth Maria Bispo; Firmo, Elza Ferreira; Oliveira, Érica Maria de; Oliveira, Sibele Ribeiro de; Rezende, Antônio Mauro; Lópes, Ana Catarina de Souza

doi:10.1590/0037-8682-0526-2019

Cited by 12 publications

(7 citation statements)

References 16 publications

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“…The non-Tn4401 variant of the present study resembles the NTE KPC− IId variant (Figure 2). These ndings corroborate the results obtained by our research group that demonstrated the presence of the bla KPC−2 gene in the non-Tn4401 element (NTE KPC− IId) [18,30], which is transported by small, mobilized and promiscuous plasmids of the IncQ1 type. These data indicate that this type of plasmid may have been responsible for the spread of the bla KPC−2 gene among K. pneumoniae in hospital patients in Recife, Brazil.…”

Section: Discussionsupporting

confidence: 92%

Ocurrence of Blandm-7 And Association With Blakpc-2, Blactx-M15, Aac, Aph, Mph(A), Catb3 And Virulence Genes In A Clinical Isolate of Klebsiella Pneumoniae With Different Plasmids In Brazil.

Alves

Scavuzzi

Beltrão

et al. 2021

Preprint

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To characterize phenotypically and genotypically an isolate of Multidrug Resistant (MDR) K. pneumoniae from a patient with septicemia in a hospital in Recife-PE, Brazil, resistance and virulence genes were investigated by using PCR and sequencing the amplicons and the plasmid DNA was also sequenced. The K74-A3 isolate was resistant to all β-lactams, including carbapenems, as well as to aminoglycosides and quinolones. By conducting a PCR analysis and sequencing, the variant blaNDM-7 associated with blaKPC-2 and the cps, wabG, fimH, mrkD and entB virulence genes were identified. The analysis of plasmid revealed the presence of blaCTX-M15, aac(3)-IVa, aph(3')-Ia, aph(4)-Ia, aac(6')ib-cr, mph(A) and catB3, and also the plasmids IncX3, IncFIB, IncQ1, ColRNAI and ColpVC. To our knowledge, this is the first report of the blaNDM-7 gene in Brazil and we suggest that this variant is located in IncX3. These results alert us to the risk of spreading an isolate with a vast genetic arsenal of resistance, in addition to which several plasmids are present that favor the horizontal transfer of these genes.

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Section: Discussionsupporting

confidence: 92%

Ocurrence of Blandm-7 And Association With Blakpc-2, Blactx-M15, Aac, Aph, Mph(A), Catb3 And Virulence Genes In A Clinical Isolate of Klebsiella Pneumoniae With Different Plasmids In Brazil.

Alves

Scavuzzi

Beltrão

et al. 2021

Preprint

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“…Contrary to previous studies reporting IncF plasmid group in E.coli to carry resistance and virulence genes often [34] the present study, however shows IncQ1 carried the highest number of both resistance and virulence genes in E.coli, but the finding is in line with study conducted in Brazil [35,36]. This is possibly due to the fact that IncQ1 plasmids have high-level mobility, stability, replication at high copy number and transferred in wide range of bacterial species through conjugative plasmids [37][38][39][40].…”

Section: Discussionsupporting

confidence: 84%

“…But the finding is in line with study conducted in Brazil [35,36]. This is possibly due to the fact that IncQ1 plasmids have high-level mobility, stability, replication at high copy number and transferred in wide range of bacterial species through conjugative plasmids [37][38][39][40].…”

Section: Discussionsupporting

confidence: 84%

Plasmid characterization in bacterial isolates of public health relevance in a tertiary healthcare facility in Kilimanjaro, Tanzania

Sengeruan

Zwetselaar²,

Kumburu³

et al. 2022

Preprint

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Plasmids are infectious double stranded DNA molecules that are found within bacteria. Horizontal gene transfer promotes successful spread of different types of plasmids within or among bacteria species, making their detection an important task for guiding clinical treatment. We used whole genome sequenced data to determine the prevalence of plasmid replicons in clinical bacterial isolates, the presence of resistance and virulence genes in plasmids, and the relationship between resistance and virulence genes within each plasmid. All bacterial sequences were de novo assembled using Unicycler before extraction of plasmids. Assembly graphs were submitted to Gplas+plasflow for plasmid prediction. The predicted plasmid components were validated using PlasmidFinder. A total of 159 (56.2%) out of 283 bacterial isolates were found to carry plasmids, with E. coli, K. pneumoniae and S. aureus being the most prevalent plasmid carriers. A total of 27 (87.1%) combined plasmids were found to carry both resistance and virulence genes compared to 4 (12.9%) single plasmids. No statistically significant correlation was found between the number of antimicrobial resistance and virulence genes in plasmids (r =-0.25, p > 0.05). Our findings show a relatively high proportion of plasmid-carrying isolates suggesting selection pressure due to antibiotic use in the hospital. Co-occurrence of antibiotic resistance and virulence genes in clinical isolates is a public health relevant problem needing attention.

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“…Conjugative frequency was calculated as the ratio of transconjugant CFU per donor. Isolates were considered unable to transfer bla KPC-2 into the recipient species if the transfer frequency was 10 −9 or lower ( 46 – 48 ).…”

Section: Methodsmentioning

confidence: 99%

Clinical and Molecular Description of a High-Copy IncQ1 KPC-2 Plasmid Harbored by the International ST15 Klebsiella pneumoniae Clone

Martins

Nicolás

et al. 2020

mSphere

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This study provides the genomic characterization and clinical description of bloodstream infections (BSI) cases due to ST15 KPC-2 producer Klebsiella pneumoniae. Six KPC-K. pneumoniae isolates were recovered in 2015 in a tertiary Brazilian hospital and were analyzed by whole-genome sequencing (WGS) (Illumina MiSeq short reads). Of these, two isolates were further analyzed by Nanopore MinION sequencing, allowing complete chromosome and plasmid circularization (hybrid assembly), using Unicycler software. The clinical analysis showed that the 30-day overall mortality for these BSI cases was high (83%). The isolates exhibited meropenem resistance (MICs, 32 to 128 mg/liter), with 3/6 isolates resistant to polymyxin B. The conjugative properties of the blaKPC-2 plasmid and its copy number were assessed by standard conjugation experiments and sequence copy number analysis. We identified in all six isolates a small (8.3-kb), high-copy-number (20 copies/cell) non-self-conjugative IncQ plasmid harboring blaKPC-2 in a non-Tn4401 transposon. This plasmid backbone was previously reported to harbor blaKPC-2 only in Brazil, and it could be comobilized at a high frequency (10−4) into Escherichia coli J53 and into several high-risk K. pneumoniae clones (ST258, ST15, and ST101) by a common IncL/M helper plasmid, suggesting the potential of international spread. This study thus identified the international K. pneumoniae ST15 clone as a carrier of blaKPC-2 in a high-copy-number IncQ1 plasmid that is easily transmissible among other common Klebsiella strains. This finding is of concern since IncQ1 plasmids are efficient antimicrobial resistance determinant carriers across Gram-negative species. The spread of such carbapenemase-encoding IncQ1 plasmids should therefore be closely monitored. IMPORTANCE In many parts of the world, carbapenem resistance is a serious public health concern. In Brazil, carbapenem resistance in Enterobacterales is mostly driven by the dissemination of KPC-2-producing K. pneumoniae clones. Despite being endemic in this country, only a few reports providing both clinical and genomic data are available in Brazil, which limit the understanding of the real clinical impact caused by the dissemination of different clones carrying blaKPC-2 in Brazilian hospitals. Although several of these KPC-2-producer K. pneumoniae isolates belong to the clonal complex 258 and carry Tn4401 transposons located on large plasmids, a concomitant emergence and silent dissemination of small high-copy-number blaKPC-2 plasmids are of importance, as described in this study. Our data identify a small high-copy-number IncQ1 KPC plasmid, its clinical relevance, and the potential for conjugative transfer into several K. pneumoniae isolates, belonging to different international lineages, such as ST258, ST101, and ST15.

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Identification of plasmid IncQ1 and NTEKPC-IId harboring bla KPC-2 in isolates from Klebsiella pneumoniae infections in patients from Recife-PE, Brazil

Cited by 12 publications

References 16 publications

Ocurrence of Blandm-7 And Association With Blakpc-2, Blactx-M15, Aac, Aph, Mph(A), Catb3 And Virulence Genes In A Clinical Isolate of Klebsiella Pneumoniae With Different Plasmids In Brazil.

Ocurrence of Blandm-7 And Association With Blakpc-2, Blactx-M15, Aac, Aph, Mph(A), Catb3 And Virulence Genes In A Clinical Isolate of Klebsiella Pneumoniae With Different Plasmids In Brazil.

Plasmid characterization in bacterial isolates of public health relevance in a tertiary healthcare facility in Kilimanjaro, Tanzania

Clinical and Molecular Description of a High-Copy IncQ1 KPC-2 Plasmid Harbored by the International ST15 Klebsiella pneumoniae Clone

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