Identification of Polybacterial Communities in Patients with Postoperative, Posttraumatic, and Endogenous Endophthalmitis through 16S rRNA Gene Libraries

Jayasudha, Rajagopalaboopathi; Narendran, Venkatapathy; Palanisamy, M.; Prabagaran, Solai Ramatchandirane

doi:10.1128/jcm.02093-13

Cited by 22 publications

(11 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Staphylococcal isolates were predominant among patients with conjunctivitis, blepharitis and blepharoconjunctivitis diagnoses, similar to that in patients from Jimma [6], Nigeria [23], and Columbia [48]. Conversely,, CoNS isolates were more frequent in keratitis (17%) and endophthalmitis (28.6%) diagnoses, as has been reported in Uganda [49], Mexico [50], Australia [8], Pakistan [51] and India [52]. …”

Section: Discussionsupporting

confidence: 72%

Ocular bacterial infections at Quiha Ophthalmic Hospital, Northern Ethiopia: an evaluation according to the risk factors and the antimicrobial susceptibility of bacterial isolates

et al. 2017

View full text Add to dashboard Cite

BackgroundExternal and intraocular infections can lead to visual impairments, which is a major public health problem. Bacteria are the most frequent pathogens affecting ocular structures; the increasing rate of antimicrobial drug resistance is a worldwide concern. The aim of this study was to determine the occurrence of bacteria in ocular infections, their antimicrobial susceptibility patterns, and risk factors in bacterial ocular infection.MethodsA hospital based cross-sectional study was conducted from September 2015 to December 2015 at Quiha Ophthalmic Hospital, Tigray, northern Ethiopia. Ocular specimens from blepharitis, blepharoconjunctivitis, conjunctivitis, keratitis, endophthalmitis, periorbital cellulitis and dacrocystitis were collected from 270 individuals with suspected ocular infection. Data on sociodemographic and risk factors were also collected using a structured questionnaire. Data analysis was performed using SPSS version 21 and 0.05 with a corresponding 95% confidence interval (CI) was considered statistically significant.ResultsAmong 270 study subjects, 180 (66.7%) were culture positive for different bacterial isolates. The predominant bacterial isolates were Staphylococcus aureus (40, 22.2%), coagulase negative staphylococci (31, 17.2%) and Pseudomonas aeruginosa (21, 11.7%). Ocular surface disease, ocular trauma, hospitalization and cosmetic application practices were significantly associated with the occurrence of bacterial infection. Concerning antimicrobial susceptibility, most isolates were susceptible to amikacin (137, 93.2%), gentamicin (131, 89.1%) and ciprofloxacin (141, 89.2%). Overall, 40 (22.5%), 34 (19.1%) and 62 (34.8%) isolates were resistant to one, two, and three or more antimicrobials, respectively.ConclusionBacteria were isolated from the majority of the study subjects. More than half of the bacterial isolates were resistant at least to one drug and a significant rate of multidrug resistance was detected. Therefore, identification of the etiologic agent and antimicrobial susceptibility testing should be practiced to select the appropriate antimicrobial agent to treat eye infections and prevent the emergence of drug resistant bacteria.

show abstract

Section: Discussionsupporting

confidence: 72%

Ocular bacterial infections at Quiha Ophthalmic Hospital, Northern Ethiopia: an evaluation according to the risk factors and the antimicrobial susceptibility of bacterial isolates

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The reported bacterial detection by eubacterial PCR assay from vitreous specimens is 56.4% (18/32 patients) from Iran,20 64% from France,16 75% (30/40) from Coimbatore, India,13 and 75.8% (44/58) from Chennai, India,21 whereas we could detect bacteria in 65.13% (127/195) by broad-range PCR assay versus 30.76% specimens by culture.…”

Section: Discussioncontrasting

confidence: 52%

“…Bacterial detection by conventional culture was reported as 16.8% (25/150 patients) from Chennai, India,4 48% (19/40) from Coimbatore, Tamilnadu,13 and 58.06% from Hyderabad,14 respectively. Conventional culture could detect bacteria from vitreous specimens in 12.2% and 25.9% (7/27) from Thailand,2 15 32% from France,16 35% (14/40) from Tehran17 and 53.84% (7/13) from Israel 3.…”

Section: Discussionmentioning

confidence: 99%

Utility of broad-range 16S rRNA PCR assay versus conventional methods for laboratory diagnosis of bacterial endophthalmitis in a tertiary care hospital

et al. 2018

View full text Add to dashboard Cite

BackgroundEndophthalmitis, a sight-threatening intraocular infection, can be of postsurgical, post-traumatic or endogenous origin. Laboratory diagnosis-based appropriate therapy can be vision-saving. Conventional culture-based laboratory diagnosis takes time and lacks sensitivity. In this study a broad-range PCR assay was assessed against conventional and automated culture methods in vitreous specimens for accurate microbiological diagnosis.AimsTo use broad-range PCR assay targeting 16S ribosomal RNA (rRNA) region of bacteria and to assess its performance vis-à-vis conventional and automated culture methods in the laboratory diagnosis of endophthalmitis.MethodsVitreous specimens from 195 patients with clinically diagnosed endophthalmitis were processed for classical and automated culture methods, antimicrobial sensitivity and broad-range PCR assay targeting 762 bp region of 16S rRNA followed by nucleotide sequencing by Sanger’s method. Causative agents were identified from the nucleotide sequences analysed against the GenBank database, and organisms were identified using the Clinical and Laboratory Standards Institute (CLSI) MM18A guidelines.ResultsBacteria could be detected from 127 (65.13%) of the 195 vitreous specimens by broad-range PCR assay; bacterial isolation was possible from 17 (8.7%) and 60 (30.76%) of these specimens by conventional and automated culture methods, respectively (p<0.0001). PCR assay could detect two uncultured bacterium, and in five cases the bacterial identity could not be determined from NCBI database matching.ConclusionBroad-range PCR assay could provide definitive microbial diagnosis within 24 hours in significantly more patients (p<0.0001). Some rare organisms could be detected, useful in treatment modalities. Automated culture was significantly more sensitive than conventional culture.

show abstract

“…Among Bacillus species, B. cereus ( Bacillus cereus ) is the major one (56.2%) followed by B. thuringiensis ( Bacillus thuringiensis ) 26.3%, B. subtilis ( Bacillus subtilis ), B. mycoides ( Bacillus mycoides ) and B. pumilis ( Bacillus pumilis ) 5.2% each; B .flexus ( Bacillus flexus ) 2.6% [ 48 ]. Less commonly, one study from India reported Clostridium species from endophthalmitis cases [ 49 ].…”

Section: Resultsmentioning

confidence: 99%

Bacterial profile of ocular infections: a systematic review

Teweldemedhin¹,

Gebreyesus²,

Atsbaha³

et al. 2017

BMC Ophthalmol

247

170

View full text Add to dashboard Cite

BackgroundBacteria are the major contributor of ocular infections worldwide. Ocular infections, if left untreated, can damage the structures of the eye with possible blindness and visual impairments. This work was aimed to review the bacterial profile of ocular infections.MethodsLiterature search was made in different electronic databases; the review was systematically made to get concrete findings.ResultsAs far as this review, Staphylococcus aureus, Coagulase negative Staphylococci, Streptococcus pneumoniae and Pseudomonas aeruginosa are the leading isolates in ocular infections. Frequent pathogens of the respective clinical diagnose include Staphylococci, Streptococcus pyogenes and Pseudomonas aeruginosa in blepharitis; Staphylococci, Streptococus pneumoniae, Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli in Conjunctivitis; Staphylococci, P. aeruginosa and E. coli in dacryocystitis; Coagulase negative Staphylococci, Pseudomonas aeruginosa and Staphylococcus aureus in keratitis; Streptococcus viridians, Streptococcus pneumoniae and Coagulase negative Staphylococci in endophthalmitis diagnoses. Endogenous endophthalmitis is associated with Klebsiella pneumoniae whereas Coagulase negative Staphylococci and Bacillus spp. are common causes of post-operative and post-traumatic endophthalmitis. However, the predominant pathogens may not be exactly same in all areas of the world, in the United States for instance, Staphylococcus aureus, Streptococcus pneumoniae and Haemophilus influenzae are the major causes of conjunctivitis.ConclusionGram positive bacteria are the major contributor of bacterial ocular infections. The distribution and proportion of bacterial isolates among clinical diagnoses varied but without exclusive anatomical restriction. To mitigate the burden of bacterial ocular infections, physicians should regard on risk reduction and comply with etiologic approach of diagnosis.

show abstract

Identification of Polybacterial Communities in Patients with Postoperative, Posttraumatic, and Endogenous Endophthalmitis through 16S rRNA Gene Libraries

Cited by 22 publications

References 38 publications

Ocular bacterial infections at Quiha Ophthalmic Hospital, Northern Ethiopia: an evaluation according to the risk factors and the antimicrobial susceptibility of bacterial isolates

Ocular bacterial infections at Quiha Ophthalmic Hospital, Northern Ethiopia: an evaluation according to the risk factors and the antimicrobial susceptibility of bacterial isolates

Utility of broad-range 16S rRNA PCR assay versus conventional methods for laboratory diagnosis of bacterial endophthalmitis in a tertiary care hospital

Bacterial profile of ocular infections: a systematic review

Contact Info

Product

Resources

About