Identification of polycyclic aromatic hydrocarbons in sugar cane soot by gas chromatography-mass spectrometry

Zamperlini, G. C. M.; Silva, M. R. S.; Vilegas, Wagner

doi:10.1007/bf02490527

Cited by 46 publications

(29 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Coincidently, such countries present a large number of wildfires and controlled fires for agricultural purposes (sources of PAH). 7 The analytical measurement of nitro-PAH has been addressed by several chromatographic methods. High performance liquid chromatography (HPLC) with both UV and electrochemical detection, 8 and the capillary gas chromatography (CGC) with flame ionization, 8,9 electron capture, 9 nitrogen and phosphorus, 9,10 and mass spectrometry [11][12][13][14] detection were largely used for determination of nitro-PAH in atmosphere or airparticulates.…”

Section: Introductionmentioning

confidence: 99%

Solid-phase extraction of nitro-PAH from aquatic samples and its separation by reverse-phase capillary liquid chromatography

Toledo¹,

Lanças²,

Carrilho³

2007

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

Neste trabalho é proposta uma metodologia analítica para extrair 1-nitronaftaleno, 2-nitrofluoreno, 9-nitroantraceno, 3-nitrofluoranteno e 1-nitropireno como compostos modelos para nitropoliaromáticos (nitro-HPA) em amostras aquáticas. A cromatografia líquida capilar com detecção UV on-column (μLC-UV) foi empregada para separar e quantificar todos os cinco nitro-HPA extraídos. Duas técnicas diferentes de extração foram testadas e comparadas: extração líquido-líquido (LLE) e extração em fase sólida (SPE) com dois diferentes tipos de sorbentes: partículas octadecilssilica (C 18 ) e estireno divinilbenzeno (XAD-2). A eluição dos analitos foi testada com diclorometano e acetonitrila. O melhor resultado de recuperação foi obtido para extração SPE -C 18 com diclorometano com valores variando de 76 a 97% e valores de desvio padrão relativo menores que 4%. As condições cromatográficas para análise foram desenvolvidas empregando uma coluna capilar (250 × 0,3 mm) empacotada com partículas C 18 de 5 μm com eluição isocrática de 65% de acetonitrila em água (v/v) com vazão de 7 μL min -1 . Os limites de detecção obtidos no sistema UV on-column (abrangendo de ~3 a 30 μmol L -1 ) são limitantes para uma aplicação ambiental, porém adequados a estudos de toxicidade.In this work, an analytical methodology to extract 1-nitronaphthalene, 2-nitrofluorene, 9-nitroanthracene, 3-nitrofluoranthene, and 1-nitropyrene aquatic samples is proposed. Capillary liquid chromatography with on-column UV detection (μLC-UV) was employed to separate and quantitate all five nitrated polycyclic aromatic hydrocarbons (nitro-PAH) extracted from water samples. Two different extraction techniques were tested and compared: liquid-liquid extraction (LLE) and solid phase extraction (SPE) with two different sorbents, octadecylsilica (C 18 ) and styrene divinylbenzene (XAD-2) particles. The elution of analytes was tested with both acetonitrile and methylene chloride. The best recovery results were obtained for SPE-C 18 extraction with the methylene chloride with values ranging from 76 to 97% with RSD values smaller than 4%. The chromatographic conditions for analysis was carried out using a 250 × 0.3 μm i.d. packed capillary column with 5 μm C 18 particles. The elution was isocratic with 65% acetonitrile in water (v/v) as mobile phase with a flow rate of 7 μL min -1. The detection limits due to on-column UV detection (ranging ~3 -30 μmol L -1 ) are limiting for environmental applications, however, they are suited for toxicological studies.

show abstract

Section: Introductionmentioning

confidence: 99%

Solid-phase extraction of nitro-PAH from aquatic samples and its separation by reverse-phase capillary liquid chromatography

Toledo¹,

Lanças²,

Carrilho³

2007

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

show abstract

“…Arbex et al [14] observed a strong correlation between the amount of smoke particles deposited in a medium city in the state of São Paulo and the 3 number of persons who needed inhalation therapy in a local hospital, indicating that sugar cane burning may cause deleterious health effects in the exposed population. Zamperlini et al [15] reported the identification of 38 compounds originating from sugar cane soot: the sixteen PAHs from EPA's priority pollutants list, some alkyl PAHs and tiophen derivatives. However, up to now there is no specific information concerning the chemical composition of the aerosol during the sugar cane burning season and the subsequent effects on the exposed population in the region For a long time the accurate identification and quantification of such trace pollutants has been a challenge for environmental science.…”

Section: Introductionmentioning

confidence: 99%

Fast chromatographic determination of polycyclic aromatic hydrocarbons in aerosol samples from sugar cane burning

Godoi

Ravindra

Godoi

et al. 2004

Journal of Chromatography A

View full text Add to dashboard Cite

Sugar cane burning in Brazil causes remarkable amounts of organic compounds to be emitted amongst which the polycyclic aromatic hydrocarbons (PAHs) represent serious health hazards.Therefore, twenty-four hour aerosol samples (< 10 m aerodynamic diameter) were collected in Araraquara city (São Paulo state) during the harvest season using a Hi-Vol sampler. PAHs were recovered using an Accelerated Solvent Extractor and analyzed by low pressure-gas chromatography-ion trap mass spectrometry (LP-GC-ITMS). The fully automated extraction process was performed in less than 25 min with a solvent consumption of approximately 20 mL.The use of a deactivated 0.6 m x 0.10 mm ID restrictor coupled to a 10 m wide-bore analytical column allowed most of the 16 PAHs in EPA's priority list to be identified and quantified in only 13 min. Concentrations of PAHs in Araraquara aerosols ranged between 0.5 ng m -3 and 8.6 ng m -3 .

show abstract

“…Dentre as várias substâncias liberadas pela combustão da palha da cana de açúcar, encontram-se os hidrocarbonetos policíclicos aromáticos (HPAs) [7]. Os HPAs constituem uma família de compostos caracterizada por possuírem 2 ou mais anéis aromáti-cos condensados [8,9].…”

Section: Artigo Articleunclassified

Otimização e validação de uma metodologia analítica para determinação de 1-hidroxipireno em urina de cortadores de cana-de-açúcar

et al. 2010

View full text Add to dashboard Cite

This study aimed to optimize and validate an analytical methodology for determination of 1-hydroxypyrene in urine of workers involved in harvesting sugar cane. The method used for determining 1-hydroxypyrene in human urine is the enzymatic hydrolysis, extraction and clean-up by solid phase extraction (SPE) and quantified by high performance liquid chromatography with fluorescence detection (HPLC / Flu). Four types of cartridges were tested to verify the percentage of recovery. Urine of sugar-cane workers (nonsmokers and of both sexes) were collected during the harvest (n = 39) and non-harvest season (n = 34) of cane sugar. The best recovery results were attributed to the C18 cartridges. They presented recovery between 79% and 108%, with a coefficient of variation between 5% and 10%. The limit of quantification was 74 ng of 1-hydroxypyrene per liter of urine. The optimized and validated methodology was used for determination of real samples. The results found in the urine of workers at harvest period ranged from 0.026 to 2.3 mol of 1-hydroxypyrene per mol creatinine. During the non-harvesting season the results ranged from 0.0023 to 0.38 mol of 1-hydroxypyrene per mol creatinine. The validated methodology proved suitable for determination of 1-hydroxypyrene in human urine. The data indicate that there is strong correlation between excretion of 1-hydroxypyrene and the periods during and between harvests of sugar cane.

show abstract

Identification of polycyclic aromatic hydrocarbons in sugar cane soot by gas chromatography-mass spectrometry

Cited by 46 publications

References 18 publications

Solid-phase extraction of nitro-PAH from aquatic samples and its separation by reverse-phase capillary liquid chromatography

Solid-phase extraction of nitro-PAH from aquatic samples and its separation by reverse-phase capillary liquid chromatography

Fast chromatographic determination of polycyclic aromatic hydrocarbons in aerosol samples from sugar cane burning

Otimização e validação de uma metodologia analítica para determinação de 1-hidroxipireno em urina de cortadores de cana-de-açúcar

Contact Info

Product

Resources

About