Identification of Portuguese traditional grapevines using molecular marker-based strategies

Barrias, Sara; Pereira, L. S.; Rocha, Sara; Sousa, Tiago Alves de; Ibáñez, Javier; Martins-Lopes, Paula

doi:10.1016/j.scienta.2023.111826

Cited by 13 publications

(11 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For example, 'Alvarelhão', 'Caitão', 'Boal Espinho', 'Cornichel Branco', 'Malvasia Branca de S. Jorge', 'Molar', 'Transâncora', and 'Verdial' were considered as minor cultivars with no certified plant material through SSR loci [112]. Multiple techniques can be applied to identify different varieties, obtaining similar results, as verified in the study [110]. In this work, SSR, SNP, and High-Resolution Melting (HRM) assays were compared, and similar identifications were reported [113].…”

Section: Genetic Enhancementsupporting

confidence: 63%

“…In this work, SSR, SNP, and High-Resolution Melting (HRM) assays were compared, and similar identifications were reported [113]. HRM analysis is a quantitative analysis of the melt curves of product DNA fragments following PCR amplification [110]. This technique allows the identification of small variations in nucleic acid sequences [110].…”

Section: Genetic Enhancementmentioning

confidence: 80%

“…cpSSR analysis allows one to follow the maternal lineage of any grapevine. Different studies mapped the genetic distribution according to the four major chlorotypes (A, B, C, and D) [106][107][108][109][110][111]. Type B, C, and D chlorotypes were detected in the Middle Eastern populations, while in the Western Mediterranean populations, chlorotype A is more dominant.…”

Section: Genetic Enhancementmentioning

confidence: 99%

“…HRM analysis is a quantitative analysis of the melt curves of product DNA fragments following PCR amplification [110]. This technique allows the identification of small variations in nucleic acid sequences [110]. In the studies of Jailon et al and Velaco et al, the genomes of an inbred line of 'Pinot Noir' (PN40024) and a heterozygous genotype (ENTAV115) used by winemakers were sequenced [114,115].…”

Section: Genetic Enhancementmentioning

confidence: 99%

See 3 more Smart Citations

Douro Vineyards: A Perspective for the Valorization and Conservation of Grapevine Genetic Resources

Sousa,

Araújo,

Sales

et al. 2024

Agronomy

View full text Add to dashboard Cite

The wine industry is one of the most important industries globally, particularly for Portugal, making a significant contribution to the Portuguese Bioeconomy. The Douro Demarcated Region (DDR) stands out as one of the largest wine-producing areas in the country. Its centuries-old culture has fostered a rich diversity, developed through vegetative and genetic breeding. Within the wine market, the highest prices for grapes are attained only under optimal edaphoclimatic conditions and when phenotypic characteristics, such as berry size and color, flowering and seed number, align favorably. Viticulture is influenced by environmental factors, diseases, and pests, impacting its economic value, profitability, and, ultimately, the employment and economic conditions of those dependent on the crops. Genetic improvement of phenotypic traits offers a faster and more cost-effective means of achieving desirable characteristics, translating into enhanced productivity and wine quality. This work focuses on presenting information about Douro grapes (region, varieties, diseases/pest, and economic value) and explores grapevine genetic diversity, along with approaches to identify genes associated with various desired traits.

show abstract

Section: Genetic Enhancementsupporting

confidence: 63%

Section: Genetic Enhancementmentioning

confidence: 80%

Section: Genetic Enhancementmentioning

confidence: 99%

Section: Genetic Enhancementmentioning

confidence: 99%

See 2 more Smart Citations

Douro Vineyards: A Perspective for the Valorization and Conservation of Grapevine Genetic Resources

Sousa,

Araújo,

Sales

et al. 2024

Agronomy

View full text Add to dashboard Cite

show abstract

“…The SNP TaqMan® genotyping assay provides significant technological advantages, including a single enzymatic step, flexible primer location in the region surrounding the SNP site, high sensitivity and specificity in DNA detection using labelled probes, and reduced analysis time ( Boccacci et al, 2020 ). HRM is a versatile post-PCR method that can be used for the identification of SNPs, SSRs and other mutations, such as insertions and deletions (INDELs), and it can be used in the field of food traceability ( Mackay et al, 2008 ; Xanthopoulou et al, 2014 ; Merkouropoulos et al, 2016 ; Villano et al, 2017 ; Pereira et al, 2017 ; Barrias et al, 2023 ). HRM technology entails qPCR amplification in the presence of a saturation dye and subsequent melting of the amplicons by progressively increasing the temperature.…”

Section: Introductionmentioning

confidence: 99%

TaqMan® and HRM approaches for SNP genotyping in genetic traceability of musts and wines

Moine,

Boccacci,

De Paolis

et al. 2024

Current Research in Food Science

View full text Add to dashboard Cite

Genetic fingerprinting reveals how traditional farming practices aided to preserve ancient table grape varieties in Almería (southeastern Spain)

Tello,

Galán,

Rodríguez‐Torres

et al. 2024

Plants People Planet

View full text Add to dashboard Cite

Societal Impact StatementTable grape production is a traditional practice in southeastern Spain, where locals have produced fresh grapes and raisins for centuries. Many of these vines are now centenary, and they represent a useful source of diversity for developing future table grape varieties with improved traits. Genetic analysis showed that many of the local varieties identified in this study were traditional varieties from Western Mediterranean countries. Others were not identified, and they might be old grape varieties of previous importance in the region. The conservation and characterization of these varieties could be key to ensuring current and future vineyard sustainability.Summary Current worldwide table grape production focuses on a reduced number of Vitis vinifera L. varieties. However, traditional farmers have grown many table grape varieties for centuries, as they provided a steady source of fresh fruit and raisins. These ancient living genotypes potentially store a genetic diversity that can be used now to ensure future grape production. Here we focused on the study of grapevines found across Almería, one of the Spanish regions with longer tradition in table grape production. After an exhaustive inspection, we located 220 old (some centenary) vines producing grapes consumed by owners or in abandoned areas no longer devoted to agriculture. Some of these vines were identified by comparing their simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) genetic profiles with available data from international databases. We found that, while grape growers' efforts focused on the cultivation of traditional grape varieties from Western Mediterranean regions, they also cultivated few exogenous varieties if they provided additional fruit features. Other vines were found to have genetic profiles that did not match reference datasets. Interestingly, some of them were found in multiple locations, suggesting they are endangered varieties with some previous relevance in the region. Besides, first‐degree relationships support the autochthonous origin of many of these unidentified genotypes. Locals kept a high number of different grapevine varieties, now considered reservoirs of genetic diversity. Traditional farming practices have been useful to prevent the loss of this diversity, which now needs to be preserved and further studied to contribute to the sustainability of viticultural systems.

show abstract

Identification of Portuguese traditional grapevines using molecular marker-based strategies

Cited by 13 publications

References 26 publications

Douro Vineyards: A Perspective for the Valorization and Conservation of Grapevine Genetic Resources

Douro Vineyards: A Perspective for the Valorization and Conservation of Grapevine Genetic Resources

TaqMan® and HRM approaches for SNP genotyping in genetic traceability of musts and wines

Genetic fingerprinting reveals how traditional farming practices aided to preserve ancient table grape varieties in Almería (southeastern Spain)

Contact Info

Product

Resources

About