Identification of possible artifacts in the association of official analytical chemists sporicidal test

Miner, Norman; Mulberry, Gayle; Starks, A N; Powers-Prather, A; Entrup, M. R.; Armstrong, Michelle; Maida, B

doi:10.1128/aem.61.4.1658-1660.1995

Cited by 9 publications

(6 citation statements)

References 8 publications

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“…Sterilants are expected to kill all microorganisms, including bacterial spores, and are used to treat devices that penetrate tissue or present a high risk if unsterile. Viable spores still attached to various materials could remain undetected by current sporicidal tests (1), resulting in overestimation of the sporicidal activity of sterilizing agents (4,7,11,12,14,15). The goal of this study was to compare the sporicidal activities of commercial liquid sterilants under manufacturer-specified conditions by using a sensitive method able to quantitatively account for the survival of all spores on contaminated carrier devices.…”

mentioning

confidence: 99%

Bacterial Spores Survive Treatment with Commercial Sterilants and Disinfectants

Sagripanti

Bonifacino

1999

Appl Environ Microbiol

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This study compared the activity of commercial liquid sterilants and disinfectants on Bacillus subtilis spores deposited on three types of devices made of noncorrodible, corrodible, or polymeric material. Products like Renalin, Exspor, Wavicide-01, Cidexplus, and cupric ascorbate were tested under conditions specified for liquid sterilization. These products, at the shorter times indicated for disinfection, and popular disinfectants, like Clorox, Cavicide, and Lysol were also studied. Data obtained with a sensitive and quantitative test suggest that commercial liquid sterilants and disinfectants are less effective on contaminated surfaces than generally acknowledged.

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confidence: 99%

Bacterial Spores Survive Treatment with Commercial Sterilants and Disinfectants

Sagripanti

Bonifacino

1999

Appl Environ Microbiol

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“…The protein content of TTRP was 45.88% as determined by Kjeldahl method (Miner et al, 1995). TTRP was dissolved in ultrapure water at a protein concentration of 3% (w/v) and digested by seven different enzymes (papain, neutral protease, trypsin, alkaline protease, avor protease, acid protease and pepsin) for 1h under their optimal conditions (shown in Table S1), respectively.…”

Section: Preparation Of Enzymatic Hydrolysates and Screening Of The P...mentioning

confidence: 99%

Preparation of angiotensin I- converting enzyme (ACE) inhibitory peptides from Tieguanyin tea residue protein using two-step enzymatic hydrolysis

LUO

Lin

et al. 2022

Preprint

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Angiotensin I-converting enzyme inhibitory (ACEI) peptides, released from precursor proteins in vitro and in vivo, have been paid increased attention in the prevention of high blood pressure. In this study, we attempted to prepare novel ACEI peptides from Tieguanyin tea residue protein (TTRP). Different proteases were used to hydrolyze TTRP to prepare ACEI peptides, neutral protease and alkaline protease hydrolysates exhibited higher ACEI activity and was chosen in further hydrolysis treatment. Response surface methodology (RSM) was used to optimize the enzymatic condition, and the results indicated that the optimal conditions of enzymatic hydrolysis were: 4% (W/V) of TTRP in water was hydrolyzed with 3500 U/g neutral protease at 50 ℃ and pH 6.5 for 2.5 h in the first step and then with 5000 U/g alkaline protease at 50 ℃ and pH 10.0 for 3 h in the second step༎Under the optimum condition, the ACE inhibitory rate of the final products was 88.45%±0.45% at the concentration of 1.0 mg/mL. Moreover, ACEI peptides derived from TTRP showed good stability under different temperatures, pHs, metal ions and gastrointestinal digestion. These results indicated that the TTRP has potential to be used for preparing ACEI peptides, providing the basis for improving the utilization efficiency for blood pressure reduction and benefit in economic value.

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“…The methods are labourintensive and laborious. They are also known to be prone to errors 15,16 that are a cause for concern in regulatory mandated testing and have led to the development of modified methods in an attempt to remedy the situation 17 . Here much smaller test surfaces (coupons) are used and the testing is completed in micro-centrifuge tubes to facilitate ease of processing.…”

Section: Under the Microscopementioning

confidence: 99%

Antimicrobial testing of healthcare and industrial products

Priscott¹

2010

Microbiol. Aust.

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For the industrial microbiologist the testing landscape comprises a plethora of standardised testing methods. Often these are to be followed rigorously according to regulatory guidelines or rules. Sometimes the product or application may require some modification of a standard method to achieve a more realistic test result. Very occasionally, a method must be developed from scratch, based upon scientific principles and, where possible, utilising approaches used in either published papers or standards. Industrial testing is usually undertaken for one of two reasons, namely development work for new products or to satisfy regulatory requirements in order to market the product. This discussion will emphasise healthcare products, other than antibiotics, but similar principles will apply to other product categories also, albeit often to a lesser degree of testing.

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Identification of possible artifacts in the association of official analytical chemists sporicidal test

Cited by 9 publications

References 8 publications

Bacterial Spores Survive Treatment with Commercial Sterilants and Disinfectants

Bacterial Spores Survive Treatment with Commercial Sterilants and Disinfectants

Preparation of angiotensin I- converting enzyme (ACE) inhibitory peptides from Tieguanyin tea residue protein using two-step enzymatic hydrolysis

Antimicrobial testing of healthcare and industrial products

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