Identification of Possible Virulence Marker from<i>Campylobacter jejuni</i>Isolates

Harrison, James W.; Dung, Trần Thị Ngọc; Siddiqui, Fariha; Korbrisate, Sunee; Bukhari, Habib; Phan, My V. T.; Hoàng, Nguyễn Văn Minh; Carrique-Mas, Juan; Bryant, J L; Campbell, James I.; Studholme, David J.; Wren, Brendan W.; Baker, Stephen; Titball, Richard W.; Champion, Olivia L.

doi:10.3201/eid2006.130635

Cited by 44 publications

(59 citation statements)

References 11 publications

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“…The T6SS positive strain Cj255 (Harrison et al . ) was used as a positive control in this study. We found the prevalence of the T6SS marker genes in Camp.…”

Section: Resultsmentioning

confidence: 99%

“…jejuni isolates harbouring the hcp marker have been shown to be associated with chickens (Harrison et al . ). Our results show that Camp.…”

Section: Discussionmentioning

confidence: 97%

“…In a previous study, hcp is reported to be significantly associated with isolates from patients who present with a bloody diarrhoea (Harrison et al . ). However, in our study all human isolates, including 14 bloody diarrhoeal isolates, we tested were negative for the presence of hcp , icm F, vgr G suggesting they lacked T6SS.…”

Section: Discussionmentioning

confidence: 97%

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Molecular detection identified a type six secretion system in Campylobacter jejuni from various sources but not from human cases

et al. 2015

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“…The T6SS positive strain Cj255 (Harrison et al . ) was used as a positive control in this study. We found the prevalence of the T6SS marker genes in Camp.…”

Section: Resultsmentioning

confidence: 99%

“…jejuni isolates harbouring the hcp marker have been shown to be associated with chickens (Harrison et al . ). Our results show that Camp.…”

Section: Discussionmentioning

confidence: 97%

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Molecular detection identified a type six secretion system in Campylobacter jejuni from various sources but not from human cases

et al. 2015

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“…The existence of a putative functional T6SS in H. pullorum was recently proposed by Sirianni et al, who correlated the mechanism of action of this cluster with the transcellular invasion of Caco-2 intestinal epithelial cells by this pathogen (41). In C. jejuni, the prevalence of the T6SS varies greatly among isolates and appears to depend on the geographic origin of the strain, being more prevalent among strains from Asia (from 60.6% to 71.5%) than those from Western countries (Ͻ10%) (40,42). In addition, the T6SS is associated with more severe forms of C. jejuni infection, such as bloody diarrhea and bacteremia, supporting the contribution of the system to the virulence of this pathogen (40,42).…”

Section: Figmentioning

confidence: 99%

“…In C. jejuni, the prevalence of the T6SS varies greatly among isolates and appears to depend on the geographic origin of the strain, being more prevalent among strains from Asia (from 60.6% to 71.5%) than those from Western countries (Ͻ10%) (40,42). In addition, the T6SS is associated with more severe forms of C. jejuni infection, such as bloody diarrhea and bacteremia, supporting the contribution of the system to the virulence of this pathogen (40,42). Therefore, the presence of a complete and most likely functional T6SS in three out of the four H. pullorum isolates suggests that this system is common among this species, constituting an important virulence mechanism.…”

Section: Figmentioning

confidence: 99%

Helicobacter pullorum Isolated from Fresh Chicken Meat: Antibiotic Resistance and Genomic Traits of an Emerging Foodborne Pathogen

Borges

Santos

Correia

et al. 2015

Appl Environ Microbiol

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gMeat and meat products are important sources of human intestinal infections. We report the isolation of Helicobacter pullorum strains from chicken meat. Bacteria were isolated from 4 of the 17 analyzed fresh chicken meat samples, using a membrane filter method. MIC determination revealed that the four strains showed acquired resistance to ciprofloxacin; one was also resistant to erythromycin, and another one was resistant to tetracycline. Whole-genome sequencing of the four strains and comparative genomics revealed important genetic traits within the H. pullorum species, such as 18 highly polymorphic genes (including a putative new cytotoxin gene), plasmids, prophages, and a complete type VI secretion system (T6SS). The T6SS was found in three out of the four isolates, suggesting that it may play a role in H. pullorum pathogenicity and diversity. This study suggests that the emerging pathogen H. pullorum can be transmitted to humans by chicken meat consumption/contact and constitutes an important contribution toward a better knowledge of the genetic diversity within the H. pullorum species. In addition, some genetic traits found in the four strains provide relevant clues to how this species may promote adaptation and virulence. Infectious diarrhea is a major cause of morbidity and mortality throughout the world, particularly in children (1). Besides the most commonly associated pathogens, such as Salmonella enterica, Campylobacter spp., and Shiga toxin-producing Escherichia coli, infectious intestinal pathogens also include Helicobacter species. Among these, Helicobacter pullorum has been detected in poultry as well as in human samples (2, 3).H. pullorum is a Gram-negative, gently curved rod, with an unsheathed monopolar flagellum (4). This enterohepatic Helicobacter species was initially isolated from the liver, duodenum, and cecum of asymptomatic poultry, but it has also been associated with enteritis and vibrionic hepatitis in broiler chickens and laying hens (4). In poultry, H. pullorum was found to colonize the cecum at high concentrations, as well as to be present on poultry carcasses, possibly due to contamination during slaughtering. Therefore, the potential role of this bacterium as an emerging foodborne human pathogen needs to be considered (3, 5-7). In humans, H. pullorum is considered an emergent agent implicated in several digestive pathologies, such as gastroenteritis (2) and chronic inflammatory conditions of the intestine (8, 9) and liver (for a review, see reference 10 and references cited therein). However, the lack of available detection tools and the difficulties in cultivating this fastidious organism result in an underdetection of the pathogen and underdiagnosis of the associated infection. Despite its zoonotic potential, there is no proof to date that one can acquire H. pullorum infection by the handling and consumption of raw or undercooked broiler chicken meat.In the present work, we report the isolation of H. pullorum from chicken meat and present antimicrobial susceptibility data and ...

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Role of putative virulence traits of Campylobacter jejuni in regulating differential host immune responses

Singh

Mallick

2019

J Microbiol.

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Identification of Possible Virulence Marker fromCampylobacter jejuniIsolates

Cited by 44 publications

References 11 publications

Molecular detection identified a type six secretion system in Campylobacter jejuni from various sources but not from human cases

Molecular detection identified a type six secretion system in Campylobacter jejuni from various sources but not from human cases

Helicobacter pullorum Isolated from Fresh Chicken Meat: Antibiotic Resistance and Genomic Traits of an Emerging Foodborne Pathogen

Role of putative virulence traits of Campylobacter jejuni in regulating differential host immune responses

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