Identification of potato chromosomes with Giesma

Mok, David W. S.; Lee, Heiyoung K.; Peloquin, S. J.

doi:10.1007/bf02851508

Cited by 14 publications

(4 citation statements)

References 15 publications

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“…Dr Peloquin and co-workers also developed several techniques for cytological and cytogenetic research that are also useful for classroom teaching. Among these are methods for germinating potato pollen on artificial media (Mortenson et al, 1964), a modified Giesma staining technique for identifying somatic chromosomes of 2x potatoes (Mok et al, 1974), and a stain-clearing technique for observation within whole ovules of potato and thus populations of megasporocytes and megagametophytes (Stelly and Peloquin, 1985;Stelly et al, 1986b).…”

Section: Isozymes Half-tetrad Analysis and Cyto-techniquesmentioning

confidence: 99%

Ploidy manipulation of the gametophyte, endosperm and sporophyte in nature and for crop improvement: a tribute to Professor Stanley J. Peloquin (1921–2008)

et al. 2009

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Based upon a foundation of genetics, cytogenetics and plant reproductive biology, Dr Peloquin and co-workers developed methods to use 2n gametes and haploids for breeding, and used them to move genes for important horticultural traits from wild tuber-bearing Solanum species to cultivated potato for the betterment of agriculture. The resulting potato germplasm included combinations of yield, adaptation, quality and disease resistance traits that were previously unavailable. This elite plant germplasm was utilized and distributed to 85 countries by the CIP, because it not only increased potato yields and quality, it also broadened the adaptation of potato to lowland tropical regions, where humanity has benefited from this addition to their food supply.

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Section: Isozymes Half-tetrad Analysis and Cyto-techniquesmentioning

confidence: 99%

Ploidy manipulation of the gametophyte, endosperm and sporophyte in nature and for crop improvement: a tribute to Professor Stanley J. Peloquin (1921–2008)

et al. 2009

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“…Molecular cytogenetics has been one of the major instruments in tracing the course of alien chromosomes in introgressive hybridization breeding. It has been applied for most major crop species (Benavente et al 2008), including potato (Yeh and Peloquin 1965;Mok et al 1974;Pijnacker and Ferwerda 1984;Visser and Hoekstra 1988;Mohanty et al 2004;Gavrilenko 2007). It allows identification of whole chromosome sets or of specific chromosome pairs and it also enables comparisons of the chromosomal positions of markers or regions of interest across related species.…”

Section: Molecular Cytogenetics Toolsmentioning

confidence: 99%

Introgressive Hybridization in Potato Revealed by Novel Cytogenetic and Genomic Technologies

2018

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Potato is the third most important food crop in the world and is crucial to ensure food security. However, increasing biotic and abiotic stresses jeopardize its stable production. Fortunately, breeders count on a rich pool of wild relatives that provide sources for disease resistance and tolerance to environmental stresses. To use such traits effectively, breeders require tools that facilitate exploration and exploitation of the genetic diversity of potato wild relatives. Introgression programs to incorporate such alien chromatin into the crop have so far relied on cytogenetic and genetic studies to tap desired traits from these wild resources. The available genetic and cytogenetic tools, supplemented with more recent genomic technologies, can assist in the use of potato relatives in pre-breeding. This information can also facilitate cisgenesis and genome editing to improve potato cultivars. Despite the abundant and rapidly growing genomic information of potato, that of its wild relatives is still limited. Resumen La papa es el tercer alimento más importante en el mundo y es crucial para garantizar la seguridad alimentaria. No obstante, el aumento de los factores adversos bióticos y abióticos pone en riesgo la estabilidad de la producción. Afortunadamente, los mejoradores cuentan con rico acervo de parientes silvestres que suministran fuentes de resistencia a enfermedades y tolerancia a factores ambientales adversos. Para utilizar tales caracteres efectivamente, los mejoradores requieren de herramientas que faciliten la exploración y explotación de la diversidad genética de los parientes silvestres de la papa. Los programas de introgresión para incorporar tal cromatina ajena al cultivo hasta ahora han recurrido a estudios citogenéticos y genéticos para captar caracteres deseables de esas fuentes silvestres. Las herramientas genéticas y citogenéticas disponibles, suplementadas con tecnologías genómicas más recientes, pueden asistir en el uso de los parientes de la papa en pre-mejoramiento. Esta información también puede facilitar la cisgénesis y la edición genómica para mejorar las variedades de papa. A pesar del abundante y rápido crecimiento de la información genómica de la papa, la de los parientes silvestres es aún limitada.

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“…Chromosomes in Solanum species are small and are similar in size. Classical banding techniques do not give a sufficient number of characteristic bands for reliable chromosomal identification (Mok et al 1974). Therefore, conventional cytogenetic techniques will not be practical for identifying S. etuberosum chromosomes in the potato background.…”

Section: Introductionmentioning

confidence: 99%

Cytological characterization of potato -Solanumetuberosumsomatic hybrids and their backcross progenies by genomic in situ hybridization

Dong¹,

Novy²,

Helgeson³

et al. 1999

Genome

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Four somatic hybrids derived from a diploid wild species Solanum etuberosum and a diploid tuber-bearing Solanum clone 463-4, together with five BC1 and three BC2 plants, were analyzed by genomic in situ hybridization (GISH). None of the four somatic hybrids had the expected chromosome constitutions, i.e., 24 chromosomes from each fusion parent. Either one chromosome from S. etuberosum or one from the potato parent 463-4 was lost in the hybrids. Three BC1 plants had exactly one set of S. etuberosum chromosomes. The other two BC1 plants either had one extra or one fewer S. etuberosum chromosome, possibly because their somatic hybrid parents had an extra or had lost one S. etuberosum chromosome. The presence of one set, or close to one set, of S. etuberosum chromosomes in all BC1 plants suggests a preferential pairing and segregation of the S. etuberosum chromosomes in the somatic hybrids. Two of the three BC2 plants had 52 chromosomes, deviating significantly from the expected chromosome number of 48. These results suggest poor pairing between S. etuberosum and S. tuberosum chromosomes in the BC1 plants. The present study demonstrates the importance of combining GISH and DNA marker analysis for a thorough characterization of potato germplasm containing chromosomes from different species.Key words: potato germplasm, Solanum etuberosum, molecular cytogenetics.

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Identification of potato chromosomes with Giesma

Cited by 14 publications

References 15 publications

Ploidy manipulation of the gametophyte, endosperm and sporophyte in nature and for crop improvement: a tribute to Professor Stanley J. Peloquin (1921–2008)

Ploidy manipulation of the gametophyte, endosperm and sporophyte in nature and for crop improvement: a tribute to Professor Stanley J. Peloquin (1921–2008)

Introgressive Hybridization in Potato Revealed by Novel Cytogenetic and Genomic Technologies

Cytological characterization of potato -Solanumetuberosumsomatic hybrids and their backcross progenies by genomic in situ hybridization

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