Identification of potato cyst nematode resistant genotypes using molecular markers

Sudha, R.; Venkatasalam, E. P.; Bairwa, Aarti; Bhardwaj, Vinay; Dalamu,; Sharma, R. C.

doi:10.1016/j.scienta.2015.11.029

Cited by 17 publications

(14 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For potatoes a successful stacking of the major R-genes involved in monogenic control of resistance to pathogens was reported in a number of papers [39,44,50,53,81]. Several studies demonstrate the potential of MAS for stacking of QTLs involved in the control of quantitative resistance to G. pallida, pathotype Pa2/Pa3 [45,82,83] as well as for pyramiding of QTLs conferring resistance to both PCN species-G. pallida, pathotype Pa2/3 and G. rostochiensis, pathotype Ro5 [41,[43][44][45][46][47][48][49][50][51][52][53][54]. The efficiency of such breeding programs can be significantly improved using marker-assisted selection.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Phenotypic and DNA Marker-Assisted Characterization of Russian Potato Cultivars for Resistance to Potato Cyst Nematodes

et al. 2021

View full text Add to dashboard Cite

Potato is one of the most important food crops in the world and also in the Russian Federation. Among harmful organisms reducing potato yield potential, the potato cyst nematodes (PCN) are considered to be ones of the most damaging pests. Information on PCN resistant cultivars is important for potato breeding and production. Russian potato cultivars are characterized in the state-bio-test program for resistance to only one PCN species Globodera rostochiensis and one pathotype Ro1 which is reported to be present in the country. This study aimed to find domestic cultivars with multiple resistances to different PCN species and different pathotypes using phenotyping coupled with molecular marker analysis due to the risk of the occasional introduction of new pests. The phenotypic response was determined by the inoculation of plants with pathotypes Ro5 of G. rostochiensis and Pa3 of G. pallida. The obtained results were supplemented by the state-bio-test data on resistance to Ro1 of G. rostochiensis. Nine of 26 Russian cultivars were resistant both to Ro5 and Ro1 pathotypes and two cultivars possess multiple resistances to both PCN species. Most tested molecular markers associated with the Gpa2, GpaVvrn, GpaVsspl, Grp1 loci showed discrepancies with phenotyping. However, a predictive haplotype and epistatic effect were detected.

show abstract

Section: Discussionmentioning

confidence: 99%

“…According to results of molecular marker analysis the majority of commercial potato cultivars which are highly resistant to the most wide-spread Ro1 pathotype of G. rostochiensis have the major H1 gene from S. tuberosum ssp. andigena while the Gro1-4 gene from S. spegazzini is much less common [36][37][38][39][40][41]43,53,54].…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and DNA Marker-Assisted Characterization of Russian Potato Cultivars for Resistance to Potato Cyst Nematodes

et al. 2021

View full text Add to dashboard Cite

show abstract

“…This presence/absence marker generates a 226 bp amplicon from resistant sources, and the diagnostic value of the SPUD1636 marker to identify sources of resistance was demonstrated for some accessions of S. vernei as well as varieties and breeding lines ( Bryan et al, 2002 ). The marker has also been adapted for MAS in a Spanish resistance breeding programme ( Ortega and Lopez-Vizcon, 2012 ) and used for resistance screening by Sudha et al (2016) . However, it has turned out to be inefficient in selecting breeding lines ( Milczarek et al, 2011 ).…”

Section: Breeding For Pcn Resistance and The Development Of Molecular Markers For Marker Assisted Breedingmentioning

confidence: 99%

Resisting Potato Cyst Nematodes With Resistance

et al. 2021

View full text Add to dashboard Cite

Potato cyst nematodes (PCN) are economically important pests with a worldwide distribution in all temperate regions where potatoes are grown. Because above ground symptoms are non-specific, and detection of cysts in the soil is determined by the intensity of sampling, infestations are frequently spread before they are recognised. PCN cysts are resilient and persistent; their cargo of eggs can remain viable for over two decades, and thus once introduced PCN are very difficult to eradicate. Various control methods have been proposed, with resistant varieties being a key environmentally friendly and effective component of an integrated management programme. Wild and landrace relatives of cultivated potato have provided a source of PCN resistance genes that have been used in breeding programmes with varying levels of success. Producing a PCN resistant variety requires concerted effort over many years before it reaches what can be the biggest hurdle—commercial acceptance. Recent advances in potato genomics have provided tools to rapidly map resistance genes and to develop molecular markers to aid selection during breeding. This review will focus on the translation of these opportunities into durably PCN resistant varieties.

show abstract

“…Из 812 сортов и селекционных клонов японского селекцентра NARO (Хоккайдо) у 33 % образцов были выявлены маркеры гена Н1, в то же время ни один из протестированных образцов не обладал маркером гена Gro1-4 (Asano et al, 2012). Аналогично в выборке из 58 се лекционных клонов индийской селекции около 60 % генотипов имели маркеры гена H1, а генотипов с маркером гена Gro1-4 не обнаружено (Sudha et al, 2016(Sudha et al, , 2019. При этом около 20 % из 217 протестированных образцов коллекции UACh Университета Южного Чили оказались MASпозитивными в скрининге с маркером гена Gro1-4 (López et al, 2015).…”

Section: G Rostochiensis (зкн)unclassified

Quarantine nematode species and pathotypes potentially dangerous for domestic potato production: populations diversity and the genetics of potato resistance

et al. 2020

View full text Add to dashboard Cite

The review considers quarantine species and nematode pathotypes potentially dangerous for domestic potato production. Potatoes are affected by more than 30 types of parasitic nematodes, but the review focuses on the most harmful representatives of genera that cause great damage to potato production: Globodera, Ditylenchus, Nacob bus and Meloidogyne. Phytopathological and molecular methods of identification of species and pathotypes and the main achievements in studying the population variability of parasitic potato nematodes were analyzed. It was shown that due to the peculiarities of the life cycle of nematodes and lability of their genomes, the genetic variability of these organisms is very high, which creates a threat of forming new pathogenic genotypes of the parasites. The information about the intra- and interpopulation variability of nematodes is important for studying the ways of introduction and distribution of separate species, as well as for searching for the correlations of molecular markers with the pathotype. Phylogenetic studies based on modern data on genetic variability of populations have allowed to reveal species complexes in Globodera pallida (Stone) Behrens and Nacobbus aberrans (Thorne) Thorne & Allen (sensu lato), including cryptic species. The main components of successful protection preventing a wide distribution of parasitic nematodes are quarantine measures, agricultural techniques, biological methods of protection and cultivation of resistant cultivars. Special attention in the review is paid to the breeding of potato cultivars with durable resistance to various nematode pathotypes, because the cultivation of such varieties is the most ecologically safe and economically advantageous way to prevent epiphytoties. Currently, significant progress has been made in the genetic protection of potato cultivars, especially against cyst-forming nematodes. The review provides data on sources of potato resistance to parasitic nematodes identified in collections of wild and cultivated species. Data on identified R-gens and QTL of resistance that have been introduced into breeding varieties using different methods and approaches are analyzed. The literature data on the study of structural and functional organization of genes for resistance to potato cyst nematodes are given. The results of molecular research on revealing the polymorphisms of loci involved in the control of resistance to cyst and gall nematodes, the development of molecular markers of certain genes and their use in marker-assisted selection for developing of new resistant cultivars, including those with group resistance, are considered.

show abstract

Identification of potato cyst nematode resistant genotypes using molecular markers

Cited by 17 publications

References 21 publications

Phenotypic and DNA Marker-Assisted Characterization of Russian Potato Cultivars for Resistance to Potato Cyst Nematodes

Phenotypic and DNA Marker-Assisted Characterization of Russian Potato Cultivars for Resistance to Potato Cyst Nematodes

Resisting Potato Cyst Nematodes With Resistance

Quarantine nematode species and pathotypes potentially dangerous for domestic potato production: populations diversity and the genetics of potato resistance

Contact Info

Product

Resources

About