Identification of potential ACE-inhibitory peptides from dromedary fermented camel milk

Alhaj, Omar A.

doi:10.1080/19476337.2016.1236353

Cited by 28 publications

(10 citation statements)

References 16 publications

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“…The study by Gomez-Guzman et al also showed that when different bacteria were used, the effect on hypertension was different [ 45 ]. Alhaj’s research also showed that the amount of ACE-inhibiting peptides produced by different strains is not consistent [ 8 ]. Probiotics release ACE-inhibiting peptides through their unique proteolytic system, which consists of cellular envelope proteases (CEPs), transport systems and intracellular peptidases.…”

Section: Discussionmentioning

confidence: 99%

“…In particular, two short peptides, Ile-Pro-Pro (IPP) and Val-Pro-Pro (VPP), are as effective as antihypertensive drugs in treating hypertension (clinical practice). In addition to milk proteins as a source of bioactive peptides, ten Ace-inhibiting peptides were also identified from dromedary milk produced by Lactobacillus helveticus or Lactobacillus acidophilus , which have good stability and can be later recovered and added to food to make food products with ACE inhibitory activity [ 8 ]. Further studies revealed that these two short peptides, IPP and VPP, may take effect by inhibiting the activity of Angiotensin Converting Enzymes (ACE).…”

Section: Introductionmentioning

confidence: 99%

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Antihypertensive Activity of Milk Fermented by Lactiplantibacillus plantarum SR37-3 and SR61-2 in L-NAME-Induced Hypertensive Rats

Yuan

Chen

et al. 2022

Foods

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Probiotic fermented milk can lower the incidence rate of hypertension and is beneficial to the regulation of the intestinal microecology. However, the underlying molecular mechanism remains elusive. Here, we evaluated the role of the gut microbiota and its metabolites in the antihypertensive effect of milk fermented by the Lactiplantibacillus plantarum strains SR37-3 (PFM-SR37-3) and SR61-2 (PFM-SR61-2) in Ng-nitro-L-arginine methyl ester induced hypertensive rats. The results showed that PFM-SR37-3 and PFM-SR61-2 intervention significantly lowered the blood pressure (BP) of NG-nitro-L-arginine methyl ester induced hypertensive rats and attenuated renal injury. In particular, long-term administration of PFM inhibited a progressive elevation in SBP (170.22 ± 8.40 and 133.28 ± 6.09 by model group and PFM-SR37-3 treated model group, respectively, at the end of the 4 weeks; p < 0.01 PFM-SR37-3 treated model group versus model group) and DBP (133.83 ± 5.91 and 103.00 ± 6.41 by model group and PFM-SR37-3 treated model group, respectively, at the end of the 4 weeks; p < 0.01 PFM-SR37-3 treated model group versus model group). PFM-SR37-3 and PFM-SR61-2 reshaped the gut microbiome and metabolome, and especially regulated the metabolic levels of L-phenylalanine, L-methionine and L-valine in the intestine and blood circulation. The analysis of the target organ’s aortic transcriptome indicated that the protective effects of PFM-SR37-3 and PFM-SR61-2 were accompanied by the modulation of the BP circadian rhythm pathway, which was conducive to cardiovascular function. Vascular transcriptomic analysis showed that circadian rhythm and AMPK might be potential targets of hypertension. In addition, the ACE inhibition rates of Lactiplantibacillus plantarum SR37-3 and Lactiplantibacillus plantarum SR61-2 in vitro were 70.5% and 68.9%, respectively. Our research provides new insights into novel and safe options for hypertension treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antihypertensive Activity of Milk Fermented by Lactiplantibacillus plantarum SR37-3 and SR61-2 in L-NAME-Induced Hypertensive Rats

Yuan

Chen

et al. 2022

Foods

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“…For analyzing the antidiabetic and ACE inhibitory activities, we have passed the water-soluble extract of fermented camel milk from 3 and 10 kDa membrane filter cut off separately for evaluating the inhibition activities. (Alhaj, 2017). The prevalence of the Cterminal aromatic amino acid, amino acid, and hydrophobic amino acid in ACE-inhibiting peptide was found to be significantly linked with its efficiency (Ma et al, 2019).…”

Section: Antidiabetic and Ace Inhibitory Effects Of Fermented Camel M...mentioning

confidence: 96%

“…It has been discovered that when L. rhamnosus was administered into camel milk, it had an ACE inhibitor effect (Moslehishad et al, 2013). (Alhaj, 2017) discovered KVLPVPQ, LHLPLPL, KVLPVPQQMVPYPQ, VLPFQEPVPDPVRG, FQEPFPDPVR, and VMVPFLQPK in Lactobacillus helveticus –fermented camel milk. Li et al (2019) also isolated three ACE‐inhibitory peptides from bovine milk fermented with L. casei KLDS and L. helveticus KLDS.31: Lys‐Ala‐Ala‐Leu‐Ser‐Gly‐Met, Lys‐Pro‐Ala‐Gly‐Asp‐Phe, Lys‐Lys‐Ala‐Ala‐Met‐A.…”

Section: Introductionmentioning

confidence: 99%

Exploring the potential of Lacticaseibacillus paracasei M11 on antidiabetic, anti‐inflammatory, and ACE inhibitory effects of fermented dromedary camel milk ( Camelus dromedaries ) and the release of antidiabetic and anti‐hypertensive peptides

Shukla¹,

Sakure

Pipaliya³

et al. 2022

Journal of Food Biochemistry

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The goal of this investigation was to find antidiabetic peptides and inhibit angiotensin converting enzyme (ACE) in Lacticaseibacillus paracasei (M11) fermented dromedary camel milk (Camelus dromedaries). According to the findings, the rate of antidiabetic activity increased along with the incubation periods and reached its peak after 48 hr of fermentation. The inhibitions of α‐amylase, α‐glucosidase, and lipase were 80.75, 59.62, and 65.46%, respectively. The inhibitory activity of ACE was 78.33%, and the proteolytic activity was 8.90 mg/mL. M11 at 0.25 mg/mL effectively suppressed LPS‐induced pro‐inflammatory cytokines and their mediators such as NO, TNF‐α, IL‐6, and IL‐1β in RAW 264.7 cells. The rate of inoculum in the optimization phase was 1.5–2.5%, and the greatest proteolytic activity was observed after 48 hr of fermentation. The investigation of the above property in the ultrafiltered fermented milk exhibited the highest antidiabetic and ACE inhibition activities in the 3 kDa than 10 kDa fractions. The molecular weight was determined employing SDS‐PAGE, and the six‐peptide sequences were identified using 2D gel electrophoresis. Due to its high proteolytic activity, the L. paracasei strain has been reported to be useful in the production of ACE‐inhibitory and antidiabetic peptides. Amino acid sequences such from ɑ1, ɑ2, and β‐caseins have been identified within fermented camel milk by searching on online databases, including BIOPEP (for antidiabetic peptides) and AHTPDB (for hypertension peptides) to validate the antidiabetic and ACE‐inhibitory actions of several peptides. Practical applications The study aims to identify antidiabetic peptides and inhibit ACE in dromedary camel milk fermented with Lacticaseibacillus paracasei M11. Maximum antidiabetic and ACE‐inhibitory actions of the fermented camel milk were observed in 3 kDa permeate fractions. Fermented camel milk significantly reduced the excessive TNF‐α, IL‐6, and IL‐1β production in LPS‐activated RAW 264.7 cells. RP‐LC/MS was used to identify 6 bioactive peptides from dromedary fermented camel milk. This fermented camel milk could be used for the management of hypertension and diabetic related problems.

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“…The inhibitory activities of ACE are considered to be an effective way to treat hypertension (Krichen et al, 2018). Many synthetic ACE inhibitors, which are used to regulate blood pressure during clinical treatment, present some safety hazards for humans, such as potential induced coughing, renal impairment, and allergic reactions (Alhaj, 2017). In the past few years, ACE inhibitors from natural sources have been investigated as alternatives to synthetic drugs as they have potentially fewer side effects (Fitzgerald and Meisel, 2000; Jiang et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Novel Angiotensin-Converting Enzyme-Inhibitory Peptides From Fermented Bovine Milk Started by Lactobacillus helveticus KLDS.31 and Lactobacillus casei KLDS.105: Purification, Identification, and Interaction Mechanisms

Zhao

Wang

et al. 2019

Front. Microbiol.

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Fermented milks with strong angiotensin I-converting enzyme (ACE)-inhibitory activity were obtained through a culture with Lactobacillus helveticus KLDS.31 and Lactobacillus casei KLDS.105 with a fermentation and storage temperature of 37 °C. Ultrafiltration fractions with a molecular weight less than 3 kDa in fermented milk whey exhibited the strongest inhibitory activity. Correspondingly, a gastrointestinal digestion experiment showed retention of the bioactivity of these fractions with pepsin and trypsin treatment. Four ACE-inhibitory peptides from fermented milk were isolated, purified by two-step reverse chromatography, and sequenced. Furthermore, the interaction mechanisms between ACE and four isolated peptides were investigated by a molecular docking method and the Independent Gradient Model. Experimental determination of IC50 was done to verify theoretical results. The inhibitory peptide interacted with ACE as follows: Lys-Pro-Ala-Gly-Asp-Phe > Lys-Ala-Ala-Leu-Ser-Gly-Met > Lys-Lys-Ala-Ala-Met-Ala-Met > Leu-Asp-His-Val-Pro-Gly-Gly-Ala-Arg.

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Identification of potential ACE-inhibitory peptides from dromedary fermented camel milk

Cited by 28 publications

References 16 publications

Antihypertensive Activity of Milk Fermented by Lactiplantibacillus plantarum SR37-3 and SR61-2 in L-NAME-Induced Hypertensive Rats

Antihypertensive Activity of Milk Fermented by Lactiplantibacillus plantarum SR37-3 and SR61-2 in L-NAME-Induced Hypertensive Rats

Exploring the potential of Lacticaseibacillus paracasei M11 on antidiabetic, anti‐inflammatory, and ACE inhibitory effects of fermented dromedary camel milk ( Camelus dromedaries ) and the release of antidiabetic and anti‐hypertensive peptides

Novel Angiotensin-Converting Enzyme-Inhibitory Peptides From Fermented Bovine Milk Started by Lactobacillus helveticus KLDS.31 and Lactobacillus casei KLDS.105: Purification, Identification, and Interaction Mechanisms

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