Identification of potential inhibitors against
            <i>Staphylococcus aureus</i>
            shikimate dehydrogenase through virtual screening and susceptibility test

Zhu, Mengfan; Qu, Jinfeng; Deng, Qi

doi:10.1080/14756366.2024.2301768

Cited by 3 publications

(1 citation statement)

References 32 publications

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“…TP53 (PDB code: 1GZH), CASP3 (PDB code: 1GFW), CASP8 (PDB code: 1F9E), and BCL2 (PDB code: 1) were obtained from the Protein PDB database ( https://www.rcsb.org/ ) under the code 1G5M, and the 3D crystal structures of PARP1 (PDB code: 3OD8) and BAX (PDB code: 6WH0) were obtained from the Protein PDB [ 30 ]. Obtain the acacia 3D structure from the TCMSP database ( https://old.tcmsp-e.com/index.php ).…”

Section: Methodsmentioning

confidence: 99%

Mechanism of acacetin regulating hepatic stellate cell apoptosis based on network pharmacology and experimental verification

Hu,

Shen,

Liu

et al. 2024

Heliyon

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Section: Methodsmentioning

confidence: 99%

Mechanism of acacetin regulating hepatic stellate cell apoptosis based on network pharmacology and experimental verification

Hu,

Shen,

Liu

et al. 2024

Heliyon

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Discovery of acetohydroxyacid synthase inhibitors as anti-tuberculosis lead compounds from natural products

Niu,

Wu,

et al. 2025

Bioorganic & Medicinal Chemistry

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Genome-wide analysis of fitness determinants ofStaphylococcus aureusduring growth in milk

Mårli,

Nordraak,

de Bakker

et al. 2024

Preprint

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Staphylococcus aureusis a major concern in the dairy industry due to its significance as a pathogen causing bovine mastitis as well as a source of food poisoning. The nutrient-rich milk environment supports bacterial growth, but the specific genetic determinants that facilitateS. aureusproliferation and persistence in milk are poorly understood. In this study, we conducted a genome-wide CRISPR interference sequencing (CRISPRi-seq) screen to identify fitness determinants essential forS. aureusgrowth and survival in milk. We identified 282 milk-essential genes, including those with key roles in DNA replication, protein synthesis, and metabolism. Comparative analysis with brain heart infusion (BHI) as growth medium, revealed 79 genes with differential fitness, highlighting specific adaptations required for growth in milk. Notably, we found that purine biosynthesis, folate cycle pathways, and metal acquisition were particularly important in this environment. Based on this, we further demonstrate thatS. aureusis more sensitive to the folate inhibitors trimethoprim-sulfamethoxazole (TMP-SMX) in milk and identify several genes whose knockdown results in hypersensitivity to TMP-SMX in milk. Additionally, our analysis showed a relatively reduced importance of cell wall components, such as teichoic acids, forS. aureusfitness in milk, which is also reflected in reduced efficiency of antimicrobials targeting teichoic acids. Together, these findings provide new insights into the genetic basis ofS. aureusfitness and antibiotic susceptibility in milk, offering directions for novel treatment strategies against bovine mastitis.

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Identification of potential inhibitors against Staphylococcus aureus shikimate dehydrogenase through virtual screening and susceptibility test

Cited by 3 publications

References 32 publications

Mechanism of acacetin regulating hepatic stellate cell apoptosis based on network pharmacology and experimental verification

Mechanism of acacetin regulating hepatic stellate cell apoptosis based on network pharmacology and experimental verification

Discovery of acetohydroxyacid synthase inhibitors as anti-tuberculosis lead compounds from natural products

Genome-wide analysis of fitness determinants ofStaphylococcus aureusduring growth in milk

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