Identification of potential protein biomarkers for early detection of pregnancy in cow urine using 2D DIGE and label free quantitation

Rawat, Preeti; Bathla, Shveta; Baithalu, Rubina Kumari; Yadav, Munna Lal; Kumar, Sudarshan; Ali, Syed Azmal; Tiwari, Anurag; Lotfan, Masoud; Naru, Jasmine; Jena, Manoj Kumar; Behere, Pradip; Balhara, Ashok Kumar; Vashisth, Rajesh; Singh, Inderjeet; Dang, Ajay Kumar; Kaushik, Jai K.; Mohanty, T. K.; Mohanty, Ashok Kumar

doi:10.1186/s12014-016-9116-y

Cited by 42 publications

(29 citation statements)

References 31 publications

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“…Rawat et al looked for early pregnancy markers (16–35 days) and examined urine samples by 2D DIGE and LC/MS/MS. The results of the DIGE experiment showed 11 proteins which were differentially expressed between examined time points – nine proteins were up‐regulated, and the obtained change was ≥1.5‐fold.…”

Section: Resultsmentioning

confidence: 99%

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Identification of protein patterns in bovine placenta at early‐mid pregnancy – Pilot studies

Ner‐Kluza

Wawrzykowski

Franczyk

et al. 2019

Rapid Comm Mass Spectrometry

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Rationale Placenta is a crucial tissue for an appropriate development of the fetus and the course of pregnancy. Its composition and structure change dynamically along pregnancy but the full pattern of these changes is not fully described in cows yet. The aim of the present study was to detect qualitative and quantitative protein profiles of bovine placenta during early‐mid pregnancy at the time of placental formation. Methods Placental tissues from healthy cows (n = 3) in early pregnancy (3–5 months) were collected at the slaughterhouse. Maternal and fetal parts were manually divided prior to homogenization. Further analysis was done in triplicates on the maternal and fetal parts separately and subjected to one‐dimensional (1D) electrophoretic separation, followed by identification of peptide maps by nanospray liquid chromatography/tandem mass spectrometry (nanoLC/MS/MS). Proteins were identified by use of the MASCOT software with the SwissProt database. Results Proteomic analysis showed more than 4000 differentially expressed proteins in maternal and fetal parts of placenta. Each part expressed around 900 proteins, of which ca. 90 were common. The identified proteins were analyzed in accordance to molecular function and their participation in biological processes. Conclusions The obtained results provide new insight into the knowledge about biochemical characteristics of placenta (new proteins) and serve for further studies on the possible markers of physiological/pathological pregnancy or function of placenta. Moreover, our data can be a good starting point for further studies on the processes underlying the attachment of placenta.

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Section: Resultsmentioning

confidence: 99%

“…The available literature covers a description of protein profiles during 8-28 days of pregnancy in distinct matrices like plasma, 3 uterine fluids, 4,5 urine 6 and, to a limited extent, placental tissues. 7 The obtained results indicate that the pattern is very dynamic and is related to the development of fetus and progress in pregnancy.…”

Section: Introductionmentioning

confidence: 99%

Identification of protein patterns in bovine placenta at early‐mid pregnancy – Pilot studies

Ner‐Kluza

Wawrzykowski

Franczyk

et al. 2019

Rapid Comm Mass Spectrometry

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“…Pregnancy is characterized by the growth and development of an allograft foreign body in the mother's body, which leads to the activation of the maternal defence system, inducing endopeptidases. Simultaneously, there is stimulation of protease inhibitors, which protect the embryo against proteolysis and the immune response of the mother (Rawat et al, ). The protease inhibitor proteins include alpha‐2‐macroglobulin (AMBP).…”

Section: Discussionmentioning

confidence: 99%

“…Simultaneously, there is stimulation of protease inhibitors, which protect the embryo against proteolysis and the immune response of the mother (Rawat et al, 2016). The protease inhibitor proteins include alpha-2-macroglobulin (AMBP).…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of amniotic and allantoic fluid from buffaloes during foetal development

Boy

Codognoto

Faleiros‐Lima

et al. 2019

Reprod Domestic Animals

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The objective of this study was to describe the dynamic changes in protein composition and protein abundance in amniotic and allantoic fluids from buffaloes during gestation. Amniotic and allantoic fluids were collected during the first, second and third trimesters of gestation. The foetuses were measured and weighed. Fluid samples were centrifuged at 800 g for 10 min and then at 10,000 g for 60 min at 4°C. The supernatant was collected to determine the total protein concentration. Based on total protein concentration, an aliquot (50 μg) was used for in‐solution tryptic digestion, and mass spectrometry analysis (nano‐LC‐MS/MS) was performed. A multivariate statistical analysis of the proteomic data was conducted. Across the different stages of buffalo gestation, fifty‐one proteins were found in the amniotic fluid, and twenty‐one were found in the allantoic fluid. A total of twelve proteins were common among the stages, and four presented significant differences (VIP score α > 1). Fibronectin and alpha‐1‐antiproteinase were more abundant in the amniotic fluid than in the allantoic fluid. Alpha‐2‐macroglobulin and alpha‐2‐HS‐glycoprotein were more abundant in the allantoic fluid than in the amniotic fluid. Alpha‐2‐macroglobulin participates in remodelling and growth of the uterus at beginning of the gestation (first trimester), and these findings indicate that can serve as a potential tool for the early diagnosis of pregnancy in buffaloes.

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“…Available literature mainly describes implantation period in cows, as it is crucial for maternal recognition of pregnancy and endometrium receptivity. Available results provide data describing changes in plasma, uterine fluids, urine, and, to the limited extent, placental tissues during the period between 8 and 28 days of bovine pregnancy. Placentation itself, because of a more complicated sample collection, is less recognized …”

Section: Introductionmentioning

confidence: 99%

2D Electrophoretic pattern of bovine placental proteins during early‐mid pregnancy

et al. 2020

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The Placenta, like every tissue, possesses its own characteristic protein profile, which may change within the course of pregnancy. These changes can be used for the elucidation of the mechanisms related to both physiology of pregnancy and pathological events. The aim of the study was to describe proteinergic profiles of maternal and fetal parts of bovine placenta during early‐mid pregnancy by the use of 2D electrophoresis and MALDI TOF/TOF MS identification to evaluate dynamics of the possible changes necessary for placentation. Placental samples were collected from six pregnant cows (3‐5 months) in the local abattoir. Placentomes were separated, and proteins were extracted and subjected to 2D electrophoresis and MALDI TOF/TOF identification. Out of 907 spots identified by the statistical analysis of gels, 54 were identified. Out of this number, 36 spots were significantly different between examined samples. Moreover, the obtained patterns differed between maternal and fetal parts of the placenta with regard to the intensity of staining, suggesting quantitative differences in protein content. These preliminary results are unique for this period of pregnancy. Such data are important for further experiments to obtain full protein profiles necessary to understand biochemical mechanisms underlying the attachment between fetal and maternal parts of the placenta during placentation. Moreover, the outcomes may help in elucidating pregnancy biomarkers in the future.

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Identification of potential protein biomarkers for early detection of pregnancy in cow urine using 2D DIGE and label free quantitation

Cited by 42 publications

References 31 publications

Identification of protein patterns in bovine placenta at early‐mid pregnancy – Pilot studies

Identification of protein patterns in bovine placenta at early‐mid pregnancy – Pilot studies

Proteomic analysis of amniotic and allantoic fluid from buffaloes during foetal development

2D Electrophoretic pattern of bovine placental proteins during early‐mid pregnancy

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