Identification of Potential Therapeutic Targets in Malignant Mesothelioma Using Cell-Cycle Gene Expression Analysis

Romagnoli, Solange; Fasoli, Ester; Vaira, Valentina; Falleni, Monica; Pellegrini, Caterina; Catania, Anna; Roncalli, Massimo; Marchetti, Antonio; Santambrogio, Luigi; Coggi, Guido; Bòsari, Silvano

doi:10.2353/ajpath.2009.080721

Cited by 52 publications

(48 citation statements)

References 48 publications

(41 reference statements)

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“…Total RNA, isolated from 20 cases of NSCLC and matched normal tissue using reagent (Trizol; Invitrogen), was reverse transcribed 17 and analyzed for changes in Scrib mRNA expression relative to ␤2-microglobulin using TaqMan gene expression assays (Hs00363005_m1 and Hs99999907_m1, respectively; Applied Biosystems, Life Technologies Corporation, Carlsbad, CA), according to the 2 -⌬Ct formula.…”

Section: Real-time Rt-pcrmentioning

confidence: 99%

Aberrant Overexpression of the Cell Polarity Module Scribble in Human Cancer

Vaira¹,

Faversani²,

Dohi³

et al. 2011

The American Journal of Pathology

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Human Scribble (Scrib) is an evolutionary-conserved cell polarity protein, but its potential role in human cancer is controversial. Herein, we show that Scrib is nearly universally overexpressed in cultured tumor cell lines and genetically disparate cancer patient series compared with matched normal tissues in vivo. Instead of a membrane association seen in normal epithelia, tumor-associated Scrib is mislocalized and found predominantly in the cytosol. Small-interfering RNA silencing of Scrib in model lung adenocarcinoma A549 cells inhibited cell migration in wound-healing assays, suppressed tumor cell invasion across Matrigelcoated inserts, and down-regulated the expression of cell motility markers and mediators of epithelial-mesenchymal transition. These data uncover a previously unrecognized exploitation of Scrib for aberrant tumor cell motility and invasion, thus potentially contributing to disease progression in humans.

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Section: Real-time Rt-pcrmentioning

confidence: 99%

Aberrant Overexpression of the Cell Polarity Module Scribble in Human Cancer

Vaira¹,

Faversani²,

Dohi³

et al. 2011

The American Journal of Pathology

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“…Little is known about the regulation of the CAK complex though cyclin H [24,25]. Immunohistochemistry and real time quantitative polymerase chain reaction were used to determine cyclin expression and gene amplification in 219 tumors; the results indicated that cyclin H was overexpressed in all tumors [26][27][28]. These results mentioned above indicated that several biomarkers for cancer diagnosis can be found in a 2D-PAGE gel according to the approach describes hereto.…”

Section: Differential Protein Identificationmentioning

confidence: 79%

Biomarkers of liver-cancer discovered from the male patients crude serum without depletion of high abundance proteins

Luo¹,

Lin²,

Zeng³

et al. 2018

Cancer Rep Rev

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High abundance proteins (HAP) were often removed from crude serum sample when finding biomarkers of diagnosing cancers, however, it will surely filtrate many potential biomarkers bound to HAP. In order to enable the detection of these potential biomarkers, saving the HAP will be of great significance for finding more biomarkers. Here, a serum proteomics technology was developed for finding biomarkers of liver-cancer from crude human serum without depletion of HAP. The crude human serum (CHS) was dispersed in a lysis buffer that has capacities for improving the protein resolution, and then separated with two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) for proteomic analysis. The differentially expressed proteins from Crude male serum (CMS) compared to crude serum of male patient with liver cancer (LCMPCS) were found and identified by a combined off-line approach of 2D-PAGE and matrix-assisted laser desorption/ ionization-time of flight mass spectrometry (MALDI-TOF). Approximately 800 protein spots on a 2D-PAGE gel were detected by Melanie 4 software from samples both CMS and LCMPCS in the present of the lysis buffer. Significantly different expression of 24 proteins were detected in the LCMPCS compared to that in the CMS. Among these proteins, fifty were found up-regulated and eleven were found down-regulated. Most of these differently regulated proteins were identified by PMF and database search. These differential expression proteins were reported participating in some key pathway or biology process in cancer cells, indicating that they may present a biomarkers profile and may be helpful for liver-cancer diagnosis.

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“…CDK1 knockdown could provide a novel therapeutic approach to arrest cell-cycle progression in MPM cells: in 2009 it has been observed that CDC2 silencing increased the apoptotic fraction of MPM cells (85) and in 2014, the RNA-interference screening performed on MPM cell-lines confirmed its role in cell-cycle and in the apoptosis (86). CDC2 inhibition with roscovitine reduced MPM cells growth and sensitised cells to cisplatin by 2.5 to 4 fold (86).…”

Section: Clinical Implications Of Overexpressed Genes In Mpmmentioning

confidence: 99%

Overexpressed genes in malignant pleural mesothelioma: implications in clinical management

2018

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Malignant pleural mesothelioma (MPM) is a very aggressive cancer poorly responsive to current therapies. MPM patients have a very poor prognosis with a median survival of less than one year from the onset of symptoms. The biomarkers proposed so far do not lead to a sufficiently early diagnosis for a radical treatment of the disease. Thus, the finding of novel diagnostic and prognostic biomarkers and therapeutic targets is needed. Gene overexpression has been frequently associated with a malignant phenotype in several cancer types; therefore the identification of overexpressed genes may lead to the detection of novel prognostic or diagnostic marker and to the development of novel therapeutic approaches, based on their inhibition. In the last years, several overexpressed genes have been identified in MPM through gene expression profiling techniques: among them it has been found a group of 51 genes that resulted overexpressed in more than one independent study, revealing their consistency among studies. This article reviews the clinical implications of confirmed overexpressed genes in MPM described so far in literature.

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Identification of Potential Therapeutic Targets in Malignant Mesothelioma Using Cell-Cycle Gene Expression Analysis

Cited by 52 publications

References 48 publications

Aberrant Overexpression of the Cell Polarity Module Scribble in Human Cancer

Aberrant Overexpression of the Cell Polarity Module Scribble in Human Cancer

Biomarkers of liver-cancer discovered from the male patients crude serum without depletion of high abundance proteins

Overexpressed genes in malignant pleural mesothelioma: implications in clinical management

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