Identification of potentially pathogenic bacteria from tilapia (Oreochromis niloticus) and channel catfish (Clarias batrachus) culture in Samarinda, East Kalimantan, Indonesia

Hardi, Esti Handayani; Nugroho, Rudy Agung; Saptiani, Gina; Sarinah, Ria; Agriandini, Maulina; Mawardi, Mira

doi:10.13057/biodiv/d190215

Cited by 24 publications

(25 citation statements)

References 11 publications

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“…aureus was isolated from 26.6% of the examined Tilapia (O. niloticus) samples (Table4). Nearly similar results were reported by (Hardi et al, 2018); 24.32%.Higher incidence (40%)was reported by Maysoon(2014) Other contamination sources of S. aureus are soil, water, dust and air (Hanson et al, 2011). Salmonella entericais a principal cause of enteric diseases in human and animal with millions of sickness worldwide, whereas the non-typhoidal Salmonella species as a zoonotic agent is also principally associated with food borne infections (Van et al, 2012).Salmonella spp was failed to be isolated from the examined samples of Nile Tilapia (O. niloticus), Catfish (Clarias gariepinus) and Common carp (Cyprinus carpio).Similar results were recorded by Valenzuela-Armenta et al (2018), who failed to detect Salmonella in adult or fry samples of tilapia.…”

Section: Discussionsupporting

confidence: 68%

Monitoring of some Pathogenic bacteria in Nile fish

Beltagy¹,

Saad²,

Hassanien³

et al. 2020

Benha Veterinary Medical Journal

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The present study was designed to monitor the incidence of pathogenic bacteria in Nile fish. Ninety random samples of fresh Nile fish Oreochromis niloticus (Nile tilapia), Clarias gariepinus (African catfish) and Cyprinus carpio (Common carp) (30 samples of each) were randomly and periodically collected from different markets in Gharbia governorate, Egypt. They were packaged and marked individually in polyethylene bags, and bacteriologically and serologically examined. Our result showed that the incidence of E.coli was70% (n=21), 34% (n=10) and 73% (n=22) from Nile tilapia, catfish and carp, respectively. E .coli isolates From Nile tilapia (four),catfish (three) and carp (three)were serotyped. Serological identification revealed that the isolates from Nile tilapiawere (O84, O26, O128 and O119), from catfish were (O84, O26 and O128) and from carp were (O17, O128 and O119). The incidence of S. aureus were 27% (n=8), 60% (n=18) and 67% (n=20) from Nile tilapia, catfish and carp, respectively. Salmonella was failed to be detected in any of the examined fish samples. The incidence of Aeromonas spp were 80% (n=24), 100% (n=30) and 93% (n=28) from Nile tilapia, catfish and common carp, respectively. The incidence of Pseudomonas spp were93%(n=28), 100% (n=30) and 53% (n=16) from Nile tilapia, Catfish and Common carp, respectively. It was concluded that Nile fish are contaminated with many food poisoning bacteria.

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Section: Discussionsupporting

confidence: 68%

Monitoring of some Pathogenic bacteria in Nile fish

Beltagy¹,

Saad²,

Hassanien³

et al. 2020

Benha Veterinary Medical Journal

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“…Therefore, controlling these strains is pending necessary to prevent further loss of fish culture. A previous study in Indonesia by Hardi., et al (2018) reported that the Gram-negative bacteria are the most predominant bacteria found in cultured tilapia. They have isolated seven bacterial genera from tilapia and catfish (Streptococcus sp., Staphylococcus sp., Pseudomonas sp., Enterobacter sp., Aeromonas sp., Neisseria sp.…”

Section: Discussionmentioning

confidence: 93%

Isolation, phenotypic characterization and antibiotic susceptibility of prevalent bacterial pathogens implicating the mortality of cultured Nile tilapia, Oreochromis niloticus

Hassan

Abdel-Rahman

Mansour

et al. 2020

Egyptian Journal for Aquaculture

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Nile-Tilapia (Oreochromis niloticus) aquaculture represents one of the most important cultivation species in Egypt. However, Tilapia fish farming is challenged by some problems. Of those, the presence of bacterial pathogens resulting in high fish mortalities and huge economic losses. Thus, the current investigation aimed to isolate, identify, and characterize the pathogenic bacteria from Nile tilapia fish farm in El-Abassa village, Egypt and to investigate their antibiotic susceptibility as a primary step for controlling diseases. 182 bacterial isolates were obtained from one hundred Tilapia fish samples. The microbiological and biochemical analysis of the examined fish indicated the presence of only 5 bacterial genera. Three of them are Gram-negative bacteria (representing 86.26% of total isolates) including Aeromonas spp. (46.70 %), Pseudomonas spp. (23.08 %), and Vibrio spp. (16.48 %). While two genera are Gram-positive bacteria (representing 13.74% of the total isolates) including Streptococcus spp. (8.79 %) and Staphylococcus spp. (4.95 %). This indicates that Gramnegative bacteria are the main cause of high fish mortalities in the studied area while Aeromonas hydrophila exhibited the highest prevalence in infected tilapia. Antibiogram test revealed high levels of resistance expressed by all isolates to ampicillin, amoxicillin, and erythromycin. On the other hand, norfloxacin was effective against all isolated bacteria followed by ciprofloxacin; therefore, norfloxacin should be 24recommended as a supplement in fish fed-diets to control the bacterial infection. Establishing effective control methods for pathogenic isolates would greatly enhance fish production.

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“…Semakin tingginya permintaan ikan mendorong pembudidaya untuk meningkatkan produksi dengan melakukan budidaya intensif, sehingga resiko serangan penyakit pada hewan budidaya ikan juga meningkat. Penelitian pada budidaya ikan tilapia (Oreochromis niloticus), dan ikan lele (Clarias batrachus) di kota Samarinda, Kalimantan Timur ditemukan 37 jenis bakteri yang menginfeksi ikan budidaya, 14 dari bakteri tersebut diduga patogen pada manusia dan juga hewan budidaya, salah satunya ialah, Enterobacteraceae (Hardi et al, 2018).…”

Section: Pendahuluanunclassified

Pathogenicity of Bacteria Enterobacteriaceae on Zebrafish as Animal Model

2020

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Enterobacteriaceae are Gram negative bacteria contain endotoxin and exotoxins which are requirements for pathogenic bacteria and act as opportunistic pathogens. The purpose of this research was to determine the ability of Enterobacteriaceae bacteria to infect zebrafish (Danio rerio) by observing anatomical pathology, mortality, time death and cumulative time of death. The method used in this research is a completely randomized design method (CRD). This research was conducted in three stages, first preparation of zebrasfish as animal model by average size 3-5cm and reach three months old. Second, bacterial cultures from 10 species of Enterobacteriaceae were Escherichia coli 1, E. coli 2, Enterobacter cloacae 1, Acinetobacter calcoaceticus, Enterobacter amnigenus 1, Enterobacter amnigenus 1, Escherichia coli 3, Pantoea spp., E. cloacae 1, E. cloacae 2, E. cloacae 3, cultured in Brain Heart Infused Broth (BHIB) and later media washed with Phospate Buffer Saline (PBS) 0.45%. The third stage was bacterial infection to zebrafish using immersion method with each treatment repeated 3 times and observed for 120 hours. The results showed 10 species that used caused death (mortality), the highest mortality in fish that infected with E. coli 2 and E. cloacae 1 with a percentage of 66.67% infected by E. coli 2 and E. cloacae 1. Anatomical pathology in the external organs and internal organs showed post-infectious symptoms. Infection occurred with mean time to death of 12-86 hours, as long as the cumulative time of fish death caused by E. coli 2, A.cinetobacter calcoaceticus and E. cloacae 1 causing death from 24 hours after infection until the end of observation.

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Identification of potentially pathogenic bacteria from tilapia (Oreochromis niloticus) and channel catfish (Clarias batrachus) culture in Samarinda, East Kalimantan, Indonesia

Cited by 24 publications

References 11 publications

Monitoring of some Pathogenic bacteria in Nile fish

Monitoring of some Pathogenic bacteria in Nile fish

Isolation, phenotypic characterization and antibiotic susceptibility of prevalent bacterial pathogens implicating the mortality of cultured Nile tilapia, Oreochromis niloticus

Pathogenicity of Bacteria Enterobacteriaceae on Zebrafish as Animal Model

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