Identification of Pregnancy-Associated MicroRNAs in Maternal Plasma

Miura, Kiyonori; Miura, Shoko; Yamasaki, Kentaro; Higashijima, Ai; Kinoshita, Akira; Yoshiura, Koh-ichiro; Masuzaki, Hiroaki

doi:10.1373/clinchem.2010.147660

Cited by 183 publications

(190 citation statements)

References 15 publications

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“…Chim et al (2008) identified 17 out of 157 miRNAs in significantly higher concentrations in normal placentas than in maternal blood cells and the 4 most abundant placental miRNAs (miR-141, miR-149, miR-299-5p, and miR-135b) in maternal plasma samples. Similarly, in another study, Miura et al (2010) identified 24 pregnancy-associated miRNAs. However, the investigators finally selected, for further analysis, only 5 miRNAs (miR-515-3p, miR-517a, miR-517c, miR518b, and miR-526b) that showed a significant increase in maternal plasma concentrations through gestation and a significant decrease after pregnancy termination.…”

Section: Detection Of Placental-specific Mirnas In Maternal Circulationmentioning

confidence: 81%

Extracellular Nucleic Acids in Maternal Circulation as Potential Biomarkers for Placental Insufficiency

Hromadníková

2012

DNA and Cell Biology

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Section: Detection Of Placental-specific Mirnas In Maternal Circulationmentioning

confidence: 81%

Extracellular Nucleic Acids in Maternal Circulation as Potential Biomarkers for Placental Insufficiency

Hromadníková

2012

DNA and Cell Biology

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“…This step is further followed by a validation procedure, frequently RT-qPCR, with additional samples from PE cases and controls. Usually, the second step does not completely validate the profiling step (18,20,21). Thus, in order to evaluate previously differentially reported miRNAs in PE, we performed a case-control study with 23 PE patients and 44 controls.…”

Section: Discussionmentioning

confidence: 99%

“…They are critical in many biological processes, including the regulation of cell differentiation, proliferation, apoptosis, angiogenesis, and metabolism (17). Specific plasma-related miRNAs were identified in maternal plasma or serum in normal pregnancies, as well as in pathological pregnancy conditions, such as PE (18,19). Several recent reports described the significant dysregulation of specific miRNA expression in the maternal circulation of PE patients compared with controls (miR-24, miR-26a, miR-29a, miR-103, miR-130b, miR-141, miR-144, miR-152, miR-181a, miR-210, miR-342-3p, miR-520, miR-574-5p, and miR-1233) (20)(21)(22)(23).…”

Section: Introductionmentioning

confidence: 99%

Dysregulated circulating miRNAs in preeclampsia

et al. 2016

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Abstract. Preeclampsia (PE) is a pregnancy-related disease with potentially severe consequences with respect to foeto-maternal morbidity and mortality. However, the molecular pathogenesis of PE remains largely unknown. Recent reports have shown that microRNAs (miRNAs or miRs) may play important roles in the development of PE. Analysing the miRNAs in sera from preeclamptic women may improve our understanding of the pathophysiological mechanisms of the disease. The aim of this retrospective study was to identify whether circulating miRNAs were differentially expressed in PE patients compared with controls. Serum samples from 23 women who developed PE were compared with samples from 44 pregnant controls. Seventeen circulating miRNAs previously described in PE were chosen for evaluation of their expression by reverse transcription quantitative polymerase chain reaction (RT-qPCR). In the maternal serum, the miR-210-3p, miR-210-5p, miR-1233-3p, and miR-574-5p levels were found to be significantly higher in the PE patients than in the controls (P<0.05). Using a logistic regression model, we evaluated the discriminant power of those differentially expressed miRNAs, and the combination of miR-210-5p and miR-574-5p yielded an area under the curve of 0.7223 for discriminating PE patients from the controls. In conclusion, the fact that four circulating miRNAs (miR-210-3p, miR-210-5p, miR-1233-3p, and miR-574-5p) were differentially expressed in the sera of women who developed PE compared with controls confirms the possible pathophysiological role of miRNAs in PE.

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“…Although these studies provided biological insight, their clinical utility is limited by the necessity of obtaining placental tissue after delivery. Attempts to detect plasma miRNAs in maternal plasma have thus far been limited to microarray and quantitative RT-PCR approaches (28,37). Detecting placental miRNAs by deep sequencing could direct specific noninvasive diagnosis of these diseases of pregnancy.…”

mentioning

confidence: 99%

Comprehensive profiling of circulating microRNA via small RNA sequencing of cDNA libraries reveals biomarker potential and limitations

Williams

Ben‐Dov

Elias

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

325

268

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We profiled microRNAs (miRNAs) in cell-free serum and plasma samples from human volunteers using deep sequencing of barcoded small RNA cDNA libraries. By introducing calibrator synthetic oligonucleotides during library preparation, we were able to calculate the total as well as specific concentrations of circulating miRNA. Studying trios of samples from newborn babies and their parents we detected placental-specific miRNA in both maternal and newborn circulations and quantitated the relative contribution of placental miRNAs to the circulating pool of miRNAs. Furthermore, sequence variation in the placental miRNA profiles could be traced to the specific placenta of origin. These deep sequencing profiles, which may serve as a model for tumor or disease detection, allow us to define the repertoire of miRNA abundance in the circulation and potential uses as biomarkers.pregnancy | cancer

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Identification of Pregnancy-Associated MicroRNAs in Maternal Plasma

Cited by 183 publications

References 15 publications

Extracellular Nucleic Acids in Maternal Circulation as Potential Biomarkers for Placental Insufficiency

Extracellular Nucleic Acids in Maternal Circulation as Potential Biomarkers for Placental Insufficiency

Dysregulated circulating miRNAs in preeclampsia

Comprehensive profiling of circulating microRNA via small RNA sequencing of cDNA libraries reveals biomarker potential and limitations

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