Identification of prohibitin as an antigen in Behcet’s disease

Xun, Yiping; Peng, Chen; Yan, Hai; Wu, Yang; Shi, Lili; Chen, Guangyu; Du, Hongwu

doi:10.1016/j.bbrc.2014.07.126

Cited by 21 publications

(20 citation statements)

References 20 publications

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“…The purified Lamin A/C protein was electrophoresed and then transferred to polyvinylidene fluoride membrane (Merck Millipore, MA) according to standard protocols in our lab [32]. After that the membrane was blocked with 5 % nonfat milk in PBS at 37 °C for 1 h. The primary antibody (serum samples from 5 SS patients) and 5 healthy controls were diluted with 1 % nonfat milk at a dilution of 1:1000, at 4 °C for overnight.…”

Section: Methodsmentioning

confidence: 99%

Circulation autoantibody against Lamin A/C in patients with Sjögren's syndrome

Zhang

Chen

et al. 2016

Oncotarget

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Lamin A/C proteins are major components of nuclear laminae and were encoded by the LMNA gene. Recent studies have found that in addition to provides nuclear-membrane strength; it also regulates the gene expression. Lamin A/C has been confirmed as an autoantigen in RA, SLE and vasculitis. Anti-Lamin A/C antibodies also have been found by indirect immunofluorescence method. In this study, we used various research methods to confirm Lamin A/C is an autoantigen in Han Chinese patients with confirmed Sjögren's syndrome (SS). To further investigate the relationship between the autoimmune disease antigens, we compared the amino acid sequence of Lamin A/C epitope and several common antigens' antigenic determinant. As a result, we found that Lamin A/C has similar epitopes with U1RNP. It means that the potential relationship exist between Lamin A/C and U1RNP. Clinical data we collected also showed that anti-Lamin A/C and anti-U1RNP antibodies always appear in same serum sample. Therefore, we speculated that cross-reaction may take place between antigen and potential antigen, which have similar epitope. Then, by epitope spreading, the potential antigen can be a new autoantigen. Our study provided a new thinking for further research about the relationship between autoantigens and their development mechanism in autoimmune diseases.

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Section: Methodsmentioning

confidence: 99%

Circulation autoantibody against Lamin A/C in patients with Sjögren's syndrome

Zhang

Chen

et al. 2016

Oncotarget

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“…MS analyses were applied to identify results of expression of LDHA [26]. Firstly, the positive protein band on SDS-PAGE gel was excised manually and transferred to a clean 1.5 mL tube.…”

Section: Mass Spectrometrymentioning

confidence: 99%

“…The ELISAs were performed according our standard procedure with slight modifications [26][27][28]. In brief, recombinant LDHA was diluted at 100 ng/mL by dissolving in 0.05 M carbonic buffer and then added into 96-well microplate 100 μL per well.…”

Section: Clinical Validationmentioning

confidence: 99%

Lactate Dehydrogenase is involved in but not the Target Antigen in Children with Kawasaki Disease

Zhao¹,

Zhao²,

Du³

et al. 2016

J Cardiovasc Med Cardiol

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“…Self-antibodies which can activate the endothelial cells and the inflammatory response have been found to play an important role in the pathological process of BD [4]. Recently, four target antigens of AECA in BD -prohibitin, ETFB, HSP27 and annexin A2 -have been successfully identified in our laboratory [5][6][7][8]. However, more potential immune targets should be found to understand the exact pathogenesis of BD.…”

Section: Introductionmentioning

confidence: 99%

Anti-lamin A/C antibodies in patients with Behçet’s disease

Chen¹,

Yan²,

Zhang³

et al. 2018

cejoi

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Lamin a/C has been identified as a target antigen of anti-endothelial cell antibodies in vasculitis. Behçet's disease (BD) is a chronic vasculitis with unclear pathogenesis. Self-antibodies have been found to play an important role in the pathological process of BD. The aim of this study was to verify whether lamin A/C is an autoantigen of BD. First, the human lamin A/C protein was cloned and purified and verified by Western blotting. Next, a homemade ELISA kit was used to test the presence of anti-lamin A/C antibodies in BD patients. This is the first time it has been certified that lamin A/C is an autoantigen of BD. The reactivity of serum IgG against recombinant human lamin A/C was detected in 7 of 31 BD patients (23%) and 3 of 32 healthy controls (HC) (9%).

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Identification of prohibitin as an antigen in Behcet’s disease

Cited by 21 publications

References 20 publications

Circulation autoantibody against Lamin A/C in patients with Sjögren's syndrome

Circulation autoantibody against Lamin A/C in patients with Sjögren's syndrome

Lactate Dehydrogenase is involved in but not the Target Antigen in Children with Kawasaki Disease

Anti-lamin A/C antibodies in patients with Behçet’s disease

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