Identification of Protease Enzymes Involved in Biocontrol Activity

Prajapati, Vimalkumar S.; Patel, S. J.; Ray, Sanket; Patel, K. C.

doi:10.1007/978-1-0716-1724-3_43

Cited by 5 publications

(2 citation statements)

References 10 publications

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“…A single colony of the activated biocontrol bacteria was selected and connected to the chitin assay plate, prepared as previously described by Lau et al [ 68 ]. Glucanase-, protease-, and cellulase assay plates were prepared as previously described by Putri et al [ 69 ], Prajapati et al [ 70 ], and Sukmawati et al [ 71 ], respectively. A transparent circle was indicative of the bacteria being able to produce the relevant degrading enzyme.…”

Section: Methodsmentioning

confidence: 99%

Antagonism of Rhizosphere Streptomyces yangpuensis CM253 against the Pathogenic Fungi Causing Corm Rot in Saffron (Crocus sativus L.)

Wang

et al. 2022

Pathogens

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Plant diseases lead to a significant decline in the output and quality of Chinese herbal medicines. Actinomycetes play a vital role in the rhizosphere ecosystem. This is especially true for Streptomyces, which have become a valuable biological control resource because of their advantages in producing various secondary metabolites with novel structures and remarkable biological activities. The purpose of this study was to isolate an effective antagonistic actinomycete against the pathogen of corm rot in saffron. An antagonistic actinomycete, CM253, was screened from the rhizosphere soil samples of Crocus sativus, by plate co-culture with four pathogenic fungi (Fusarium oxysporum, Fusarium solani, Penicillium citreosulfuratum, and Penicillium citrinum). CM253 inhibited the growth and development of F. oxysporum hyphae by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Furthermore, by analyzing the degrading enzyme, the growth-promoting performance, and the whole genome of strain CM253, it was identified as Streptomyces yangpuensis, which produces NH3, protease, glucanase, cellulase, IAA, and ACC deaminase. In addition, 24 secondary metabolite synthesis gene clusters were predicted in antiSMASH. We identified genes encoding 2,3-butanediol; methionine; isoprene (metH, mmuM, ispEFH, gcpE, idi, and ilvABCDEH); biofilm formation; and colonization (upp, rfbBC, efp, aftA, pssA, pilD, fliA, and dhaM). Above all, S. yangpuensis CM253 showed the potential for future development as a biocontrol agent.

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Section: Methodsmentioning

confidence: 99%

Antagonism of Rhizosphere Streptomyces yangpuensis CM253 against the Pathogenic Fungi Causing Corm Rot in Saffron (Crocus sativus L.)

Wang

et al. 2022

Pathogens

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“…Since proteases can tolerate extreme conditions, and that soil microbial proteases have the capability to inhibit plant pathogens and parasites, they are seen as good biocontrol agents 24 . To assess protease production, various methods were used and one of them is growing cultures on SMA.…”

Section: Pharmaceutical Sciences Asiamentioning

confidence: 99%

Locally-isolated protease-producing Bacillus spp. from soil inhibits biofilm formation of Staphylococcus aureus

Mantaring¹,

Almazan²,

Arcan³

et al. 2023

Pharm Sci Asia

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Pathogens form biofilms to increase their resistance to environmental stress and antibacterial compounds. The rhizosphere is a rich source of microorganisms producing industrially important compounds including those with antimicrobial and biofilm inhibitory activities. Four isolates from soil collected from Taguig City, Philippines, were subjected to phenotypic and genotypic characterization, screening for protease production, and biofilm inhibition assays. Colony morphology and microscopic analyses indicated the isolates were putative Bacillus species. Upon DNA extraction, 16S rRNA gene was amplified, and based on their sequences, the isolates were confirmed to be Bacillus spp. Isolate AHP was B. cereus, isolate DJA was Priestia megaterium, formerly known as B. megaterium and isolates SJS and SKA were Bacillus spp.-all of which produced protease. Although the cell-free supernatants (CFS) of the isolates did not inhibit the growth of Staphylococcus aureus 1258, Citrobacter freundii ATCC24864, Salmonella Typhimurium ATCC13311, Escherichia coli ATCC11229, and E. coli O157:H7, biofilm formation of S. aureus was inhibited by all CFS, with B. cereus AHP showing the highest biofilm inhibition at 46%, followed by Bacillus sp. SKA (39%), P. megaterium DJA (36%), and Bacillus sp. SJS (31%). Even though further studies are warranted, the bioactivities of these isolates indicate potential use for pharmaceutical purposes due to their ability to produce protease and inhibition of biofilm formation of a common bacterial pathogen.

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Agricultural Biotechnological Potential of Bacillus velezensis C3-3 and Cytobacillus sp. T106 from Resource Islands of a Semi-arid Zone of La Guajira-Colombia

Suárez-Bautista,

Manotas-Viloría,

Leal-Mejía

et al. 2024

Curr Microbiol

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Resource islands are vegetative formations in arid and semi-arid ecosystems that harbor microorganisms facing extreme conditions. However, there is a limitation in the knowledge of the agricultural biotechnological potential of microorganisms present in these islands. This study aimed to determine the capacity of Bacillus velezensis C3-3 and Cytobacillus sp. T106 isolates from resource islands to promote plant growth and control the phytopathogen Rhizoctonia solani. The bacteria were sequenced, and both grew at 50 °C, resisted 5% NaCl, withstood UV exposure, and grew in extreme pH conditions. Sixty-six genes in C3-3 and 71 in T106 were identified associated with plant growth promotion, and C3-3 was shown to promote leaf growth in lettuce plants. This promotional effect was associated with the production of indole-3-acetic acid (IAA), phosphorus solubilization, and the presence of genes related to the assimilation of rhizosphere exudates. Both strains inhibited R. solani through the production of volatile compounds and antagonism. Forty-five and 40 of these genes in C3-3 and T106, respectively, were associated with the production of proteases, lipases, siderophores, antimicrobial compounds, degradation enzymes, and secretion systems. Notably, Cytobacillus sp. has not been previously reported as a biocontrol agent. This work contributes to the evidence of the biotechnological potential of semi-arid region bacteria, offering prospects for improving agricultural production in areas with limiting conditions.

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Identification of Protease Enzymes Involved in Biocontrol Activity

Cited by 5 publications

References 10 publications

Antagonism of Rhizosphere Streptomyces yangpuensis CM253 against the Pathogenic Fungi Causing Corm Rot in Saffron (Crocus sativus L.)

Antagonism of Rhizosphere Streptomyces yangpuensis CM253 against the Pathogenic Fungi Causing Corm Rot in Saffron (Crocus sativus L.)

Locally-isolated protease-producing Bacillus spp. from soil inhibits biofilm formation of Staphylococcus aureus

Agricultural Biotechnological Potential of Bacillus velezensis C3-3 and Cytobacillus sp. T106 from Resource Islands of a Semi-arid Zone of La Guajira-Colombia

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