Identification of protein profiles of Escherichia coli, Staphylococcus aureus and their corresponding phages

Abstract

The present study was carried out for the assessment and detecting of the Escherichia coli and Staphylococcus aureus bacteriophages and their bacterial host's proteins includes visual inspection and comparison of their molecular weight mass. Systematic methods that use microorganisms (viruses and bacteria) to test samples from the local sewage water have been developed but require some change in the classical culturing of the organism to detect it easily by naked eye through the changes in selective media. We have developed a Sodium Dodecyl Sulphate Ploy Acrylamide Gel Electrophoresis (SDS PAGE) assay for bacteriophage samples preparation that allows the rapid detection of proteins bands of these viruses by using freeze drying technique. We found that the protein profiles of E. coli bacteriophage showed three major bands with molecular weight mass of 47, 35 and 16 kilo Dalton and 34 and 20 kDa for S. aureus phage, the band of 35 kDa was the common shared peak between the phage and the bacterial host due to the bacteriophage lytic cycle. The method is suitable for use with both the bacteriophages and their bacterial hosts. The assay is rapid, sensitive, and inexpensive; requires only small sample volumes; and can be automated.

Therapeutic Potential of Staphylococcal Bacteriophages for Nasal Decolonization of <i>Staphylococcus aureus</i> in Mice

“…The results of our assessment of the purity of bacteriophages obtained by SDS-PAGE (Figure 2) were similar to those reported previously for other phage types [42,43]. It was interesting to note that the SDS-PAGE profiles of the ion-exchange-and cesium chloride-purified phages were very similar.…”

Effects of Water Treatment Methods on Protein Profile of Escherichia coli O157:H7 and Escherichia coli Non-O157 Isolated from Drinking Water Sources in Ado-Ekiti, Ekiti State, Nigeria

“…The similarity in molecular weights of their protein bands corroborates the study by Durrani et al (8) who also recorded similar molecular weights of protein bands of E. coli isolated from stool and urine samples. In a similar study, Chong et al [23] and Elshayeb et al [24] also recorded similar protein profile pattern of E. coli in their studies.…”

Identification and characterization of lytic bacteriophages specific to foodborne pathogenic Escherichia coli O157:H7

“…Φ241 phage specific to E. coli O157:H7 had 13 protein bands and four of them were major proteins whose molecular weights were the range of 26–50 kDa (Lu and Breidt, 2015). Elshayeb et al. (2011) reported that E. coli bacteriophage had three major protein bands with molecular weights of 16, 35, and 47 kDa.…”

“…(2018) reported that three bacteriophages had one major protein band (52 kDa). The molecular weights of the capsid proteins, which account for about 43.3% of the total phage proteins, are 34.3–47.7 kDa and the tail proteins constitute 12.7% of the total proteins and the molecular weights are between 22.5 and 27 kDa (Elshayeb et al., 2011; Zimmer et al., 2002).

Figure 4.SDS-PAGE gel images of E. coli O157:H7-specific bacteriophages. 1: vB_EcoM-P13; 2: vB_EcoM-P23; 3: vB_EcoM-P34; 4: vB_EcoS-P24; 5: vB_EcoM-P12; 6: marker (Bio-Rad unstained broad range protein standards).

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