2009
DOI: 10.1128/jvi.01151-09
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Identification of Proteins fromPrunus persicaThat Interact with Peach Latent Mosaic Viroid

Abstract: Peach latent mosaic viroid (PLMVd) is a small, single-stranded, circular RNA pathogen that infects Prunus persica trees. As with all other known viroids, the PLMVd genome does not encode any proteins. Consequently, it must interact with host cellular factors in order to ensure its life cycle. With the objective of identifying cellular proteins that interact with PLMVd, Northwestern hybridizations were performed using partially purified peach leaf extracts. Mass spectrometric analysis of the detected RNA-protei… Show more

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Cited by 33 publications
(17 citation statements)
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“…It is important to note that the experiments reported here were performed in the absence of any peach protein, a fact that may influence the folding of the viroid. For example, it has been shown that the presence of peach elongation factor eEF1A alters the viroid's structure near the junction between the P10 and P11 stems, adjacent to the self-cleavage site (Dubé et al 2009). Likely, this is the binding site of the elongation factor, and its binding might contribute to the viroid's polymerization by the rolling circle replication.…”
Section: Determination Of the Secondary Structure Of Plmvd Minus Stramentioning
confidence: 99%
“…It is important to note that the experiments reported here were performed in the absence of any peach protein, a fact that may influence the folding of the viroid. For example, it has been shown that the presence of peach elongation factor eEF1A alters the viroid's structure near the junction between the P10 and P11 stems, adjacent to the self-cleavage site (Dubé et al 2009). Likely, this is the binding site of the elongation factor, and its binding might contribute to the viroid's polymerization by the rolling circle replication.…”
Section: Determination Of the Secondary Structure Of Plmvd Minus Stramentioning
confidence: 99%
“…GP undergoes proteolytic cleavage by host proteases such as furin (Volchkov et al, 1998), which produces two subunits, GP1 and GP2, linked by a disulfide bond. The GP1 subunit mediates viral attachment, most likely through the MLR or the putative receptor binding region (RBR; Kuhn et al, 2006; Dube et al, 2009). The GP2 subunit has the heptad repeat regions required for assembling GP as a trimer.…”
Section: Filovirus Envelope Glycoproteinmentioning
confidence: 99%
“…BST-2 localizes both to the plasma membrane and internal compartments, particularly the trans-Golgi network (TGN) and recycling endosomes (Kupzig et al, 2003; Rollason et al, 2007; Dube et al, 2009; Masuyama et al, 2009; Habermann et al, 2010). At the cell surface, BST-2 localizes into cholesterol-enriched lipid rafts, due to its GPI anchor.…”
Section: The Gpi Anchor Mediates Surface Localization and The Ct Is Cmentioning
confidence: 99%