Identification of proteomic markers for ram spermatozoa motility using a tandem mass tag (TMT) approach

Zhu, Wen; Zhang, Yan; Ren, Chunhuan; Cheng, Xiao; Chen, Jiahong; Ge, Ze-yong; Sun, Zhirong; Zhuo, Xian; Sun, Feng-fei; Chen, Yale; Jia, Xiaojiao; Zhang, Zijun

doi:10.1016/j.jprot.2019.103438

Cited by 34 publications

(27 citation statements)

References 46 publications

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“…Deficient or abnormal proteins in the sperm tail always affect sperm motility and male fertility. 1,45 In a previous study of our laboratory, Fen (0.009375, 0.1875, 3.750, and 45.00 mg/day/kg 30 days) were found to impair sperm motility in mice, though there was no significant difference in the morphology or weight of testes between the different groups, 16 indicating that sperm motility is more vulnerable to Fen. Recent studies 9,46,47 have shown that most of the dose employed is toxic, and is higher than the ordinary exposure level in the population.…”

Section: Western Blotting Of De Proteins In Sperm and Testicular Prmentioning

confidence: 86%

“…Previous studies in our laboratory, have shown that adult mice receiving oral exposure to Fen (0.009375, 0.1875, 3.750, and 45.00 mg/day/kg 30 days) showed significant impairment in the motility parameters of path straightness (STR) and linearity (LIN) (both p < .05), though no significant damage to testicular structure or sperm count was noted. 16 This indicates that sperm motility is more vulnerable to the effect of Fen.…”

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confidence: 77%

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Key proteins of proteome underlying sperm malformation of rats exposed to low fenvalerate doses are highly related to P53

Huang

et al. 2021

Environmental Toxicology

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Fenvalerate (Fen) is an endocrine disruptor, capable of interfering with the activity of estrogen and androgen. Our objective was to explore the molecular mechanisms of Fen on sperm in vivo. Adult male Sprague-Dawley rats were orally exposed to 0, 0.00625, 0.125, 2.5, 30 mg/kg/day Fen for 8 weeks. Sperm morphology, differential proteomics of sperm and testes, bioinformatic analysis, western blotting (WB), and RT-PCR were used to explore the mechanism of Fen on sperm. Data showed that low Fen doses significantly induced sperm malformations. In sperm proteomics, 47 differentially expressed (DE) proteins were enriched in biological processes (BPs) related to energy metabolism, response to estrogen, spermatogenesis; and enriched in cellular components (CCs) relating to energy-metabolism, sperm fibrous sheath and their outer dense fibers. In testicular proteomics, 56 DE proteins were highly associated with mRNA splicing, energy metabolism; and enriched in CCs relating to vesicles, myelin sheath, microtubules, mitochondria.WB showed that the expression of selected proteins was identical to their tendency in 2D gels. Literature indicates that key DE proteins in proteomic profiles (such as Trap1, Hnrnpa2b1, Hnrnpk, Hspa8, and Gapdh) are involved in P53-related processes or morphogenesis or spermatogenesis. Also, P53 mRNA and protein levels were significantly increased by Fen; bioinformatic re-analysis showed that 88.5% DE proteins and P53 formed a complex interacting network, and the key DE proteins were coenriched with P53-related BPs. Results indicate that key DE proteins of proteome underlying sperm malformations of rats exposed to low Fen doses are highly related to P53.

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Section: Western Blotting Of De Proteins In Sperm and Testicular Prmentioning

confidence: 86%

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confidence: 77%

Key proteins of proteome underlying sperm malformation of rats exposed to low fenvalerate doses are highly related to P53

Huang

et al. 2021

Environmental Toxicology

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“…Semen from 18 (9 rams, Hu-sheep, Ovis aries; and 9 bucks, Anhui white goat, Capra hircus) mature males (1.5 ± 0.25 years) were collected by artificial vagina (April 2017) using a https://doi.org/10.1371/journal.pone.0228656.g001 previously described method [20]. Ejaculates were assessed for volume, concentration and motility immediately by a CASA system (sperm class analyzer (SCA)-5.4.0.0; Microptic Supply, Barcelona, Spain) according to Mortimer et al [21].…”

Section: Collection and Preparation Of Spermatozoamentioning

confidence: 99%

“…Western blot was performed according to our previously described method [20]. Briefly, protein samples (60 ug protein per sample) from ram and buck spermatozoa were separated prior to detection with antibodies: CRP (1:1000), DEFB1(1:1000), COII (1:1000), and ALDH2 ((1:1000) from Abcam (Abcam, Cambridge, MA, USA); NUDT18 (1:800) from BIOSS (BIOSS, Beijing, P. R. China).…”

Section: Western Blotting Validationmentioning

confidence: 99%

Proteomic characterization and comparison of ram (Ovis aries) and buck (Capra hircus) spermatozoa proteome using a data independent acquisition mass spectometry (DIA-MS) approach

et al. 2020

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Fresh semen is most commonly used in an artificial insemination of small ruminants, because of low fertility rates of frozen sperm. Generally, when developing and applying assisted reproductive technologies, sheep and goats are classified as one species. In order to optimize sperm cryopreservation protocols in sheep and goat, differences in sperm proteomes between ram and buck are necessary to investigate, which may contribute to differences in function and fertility of spermatozoa. In the current work, a data-independent acquisition-mass spectrometry proteomic approach was used to characterize and make a comparison of ram (Ovis aries) and buck (Capra hircus) sperm proteomes. A total of 2,109 proteins were identified in ram and buck spermatozoa, with 238 differentially abundant proteins. Proteins identified in ram and buck spermatozoa are mainly involved in metabolic pathways for generation of energy and diminishing oxidative stress. Specifically, there are greater abundance of spermatozoa proteins related to the immune protective and capacity activities in ram, while protein that inhibit sperm capacitation shows greater abundance in buck. Our results not only provide novel insights into the characteristics and potential activities of spermatozoa proteins, but also expand the potential direction for sperm cryopreservation in ram and buck.

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“…In the Afrino population, C14orf39 was associated with all three reproductive traits, NLB, NLW and TWW. RBMS3 was identi ed as one of the putative genes related to sperm fertility in Assaf sheep rams[47] (Serrano et al, 2019), while ELSPBP1 was differentially expressed in rams with high and low sperm motility[48] (Zhu et al, 2020). ELSPBP1 was associated with dead spermatozoa in cattle[49] (D'Amours et al, 2012) and consequently associated in various studies with spermatozoa in sub-fertile bulls[50,51] (D'Amours et al, 2010; Kumar et al, 2016).…”

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confidence: 99%

Identification of Genes Targeted in South African Merino and Afrino Sheep Populations Under Long-Term Selection for Reproduction and Body Weight

Snyman¹,

Süllwald

Olivier³

et al. 2020

Preprint

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Background: Reproductive performance and body weight are of the utmost economic importance in determining the efficiency of sheep production. Simultaneous selection for increased reproductive performance and early growth traits is a common strategy in many flocks, but ambiguous results regarding the relationship between reproduction and body weight have been reported. The objective of this study was to perform a genome-wide association study (GWAS) in two South African Merino flocks and an Afrino sheep flock that were selected for both reproduction and body weight over decades. The GWAS aimed to identify SNPs associated with genes affecting the traits number of lambs born (NLB), number of lambs weaned (NLW), total weight of lamb weaned (TWW) and body weight (BW) and thus to ascertain which genes were targeted through directional selection.Results: In the GWAS, 16 SNP markers associated with reproductive traits were identified among the three populations, while 15 SNPs were associated with body weight. These SNPs were linked respectively to 26 and 21 documented genes in the sheep genome. Most of these genes were previously associated in literature with reproduction related, as well as with growth related traits in various farm animal species. This study, supported by results from previous studies performed on sheep and cattle, identified the following genes that warrant further investigation as to their functions and processes relating to growth and reproduction in sheep: MAP7D1, TRAPPC3, THRAP3, TRMP8, SPP2, HDAC9, ZFHX3, SIX6, C14orf39, TAF4B, TRSP1 EYA2, RBMS3, STL38L, BSPH1, LIG1, CABP5 and ELSPBP1. Conclusions: Long-term selection in the flocks for both body weight and reproductive traits, and especially on the composite trait TWW, have favoured genes with pleiotropic effects influencing both groups of traits. SNPs associated with these pleiotropic genes were detected in the association analyses for the various traits.

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Identification of proteomic markers for ram spermatozoa motility using a tandem mass tag (TMT) approach

Cited by 34 publications

References 46 publications

Key proteins of proteome underlying sperm malformation of rats exposed to low fenvalerate doses are highly related to P53

Key proteins of proteome underlying sperm malformation of rats exposed to low fenvalerate doses are highly related to P53

Proteomic characterization and comparison of ram (Ovis aries) and buck (Capra hircus) spermatozoa proteome using a data independent acquisition mass spectometry (DIA-MS) approach

Identification of Genes Targeted in South African Merino and Afrino Sheep Populations Under Long-Term Selection for Reproduction and Body Weight

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