Identification of Proteus Mirabilis on Banknotes Using 16s rRNA gene in Khartoum State

Mukhtar, Alaa Abdalla; Alfadil, Noha Ahmed Abd; Mohamed, Malik Suliman; Altayb, Hisham N.; Elzaki, Salaheldein G.; Hassan, Mohamed Salih

doi:10.18502/sjms.v13i3.2955

Cited by 9 publications

(6 citation statements)

References 37 publications

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“…Our findings are similar to those obtained by Al-Imam, et al and Al-Saadi, et al who used the same genes to identify P. mirabilis and P. vulgaris [6,31]. On the other hand, Mukhtar et al and Adnan et al applied 16S rRNA PCR gene amplification technique for the identification of P. mirabilis [32,33]. The latter technique has many advantages over phenotypic and biochemical techniques.…”

Section: Isolation Culture Conditions and Identificationsupporting

confidence: 88%

Down-Regulation of fliL Gene Expression by Ag Nanoparticles and TiO2 Nanoparticles in Pragmatic Clinical Isolates of Proteus mirabilis and Proteus vulgaris from Urinary Tract Infection

Saleh¹,

Malik²,

Al-Rubaii³

2019

Nano BioMed ENG

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Ten isolates belonging to Proteus spp. were collected and obtained from Department of Biology, College of Science, the University of Baghdad. The diagnosis was done by polymerase chain reaction (PCR) technique using 16S rRNA gene and urease C gene. All isolates (100%) were sensitive to meropenem, imipenem, ciprofloxacin, gentamycin, amoxicillin, clavulanic acid and levofloxacin. These isolates also showed 60% sensitivity to cefixime and nitrofurantoin. However, both species of P. mirabilis and P. vulgaris showed the lowest sensitivity when treated with tetracycline (60%) and amikacin (20%). Cephalothin had a variable effect on the species under study as P. mirabilis isolates were 100% sensitive in comparison with the 80% sensitivity of P. vulgaris isolates. The antibacterial activities of Ag and TiO 2 nanoparticles (NPs) were investigated. The minimum inhibitory concentration (MIC) value of Ag NPs against both species isolates was 10 mg/mL, while the MIC value of TiO 2 NPs was 14 mg/mL against P. mirabilis and 15 mg/mL against P. vulgaris isolates. P. mirabilis isolates showed larger swarming diameter than P. vulgaris, but this motility phenomenon of P. vulgaris was arrested rapidly after incubation with sub-MIC of TiO 2 NPs and Ag NPs comparatively with control. All isolates showed shifting to down-regulation in the fliL gene expression under the effect of the NPs using TiO 2 NPs and Ag NPs. In conclusion, down-regulation of the fliL gene expression is directly linked to the inhibition of swarming movement of Proteus species. We encourage using these inhibitors (after tests to ensure minimal toxicity to human) in combination with antibiotics to ensure bactericidal /bacteriostatic effect to treat Proteus infections.

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Section: Isolation Culture Conditions and Identificationsupporting

confidence: 88%

Down-Regulation of fliL Gene Expression by Ag Nanoparticles and TiO2 Nanoparticles in Pragmatic Clinical Isolates of Proteus mirabilis and Proteus vulgaris from Urinary Tract Infection

Saleh¹,

Malik²,

Al-Rubaii³

2019

Nano BioMed ENG

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“…In this study, we use this gene for the identification of different isolates from soil and the human respiratory system. The 16S rRNA gene could be used as a phylogenetic marker because of its functional constancy and the presence of conserved and variable sequence regions evolving at very different rates [28] . A phylogenetic tree is a diagram that refers to evolutionary relationships among different species based on similarities and differences in their genetic characteristics.…”

Section: Discussionmentioning

confidence: 99%

Identification of Novel Bacteria by Using 16S rRNA from Clinical and Soil Samples

Enas A. Bady,

Sabaa Ali Mohammed Al-Fadal,

Rafeef Amer Abdul-Jabar

et al. 2021

Indian Journal of Forensic Medicine & Toxicology

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The 16S rRNA gene is a useful tool and molecular indication for identifying novel bacterial species from different sources. It is widespread for members of this field and thanks to the continuous expansion of information sequence databases. We isolated Gram-negative and Gram-positive bacilli from the respiratory infections and soil respectively, and phenotypical and molecular identifications were performed. Genetic analysis indicated that the isolated bacteria were a new species belonging to Bacterium strain and Proteus sp. Bacterium strain JND-RSIb-21A with Accession number MW013547.1 which closely related (99.8%) with Bacillus subtilis strain JND-RSIb-21A for its Transversion point mutation (G instead of C) at the position 445 bp. Proteus sp. strain AMJ131 with Accession number MW015095.1 the isolate related closely (99.8%) to Proteus mirabilis strain AMJ131for two-point mutations Transition of (G instead of T) and Transversion of (T instead of A) at position 9bp and 10bp respectively.

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“…The present research showed that 16S rRNA was a strong selective power to determine isolated P. mirabilis, which was compatible with the Saleh et al(2019) 16S rRNA test to recognize Proteus spp. isolated from urinary tract infection patients and the Mukhtar et al (2018) finding the 16s rRNA gene used to identify Proteus mirabilis on banknotes ,the two above research have been reported to be a strongly selective force for Proteus spp recognition by 16S rRNA [9;17]. The 16S rRNA gene, which has a length of 1.5 kilobase (kb), has proved to be a valuable molecular target because it is found in all bacteria, whether as a single copy or in multiple copies, and is strongly conserved in a species over time.…”

Section: Pcr Identificationmentioning

confidence: 99%

“…Genotypic bacterial analysis starts with the PCR product's nucleotide sequence analysis of the respective gene(s) followed by a comparison of such sequences with the identified sequences in the database [9].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Molecular Identification of proteus mirabilis isolated from hospitals in the capital Baghdad

2021

IJFMT

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Proteus mirabilis a Gram-negative bacteria belonging the Enterobacteriaceae family and that causes several infections to patients such as urinary tract infections, middle ear infections, wounds and burns, in addition to other infections. This study aimed to isolate and diagnose Proteus mirabilis bacteria collected from different samples of infection sources from patients present in many hospitals using traditional diagnostic like morphological and biochemical tests as well as molecular methods. The Identification system for Gram negative bacteria GN24 KIT had used to detect Proteus mirabilis species . The results of using this kit were confirmed by the molecular diagnosis of bacteria through the PCR technique of the 16S rRNA gene, where the confirmation rate was 100%. The PCR sample was analyzed in Macrogen Corporation-Korea for Sanger sequencing using ABI3730XL, an automated DNA sequences. the findings were obtained and analyzed via genius software in order to Synthesis of the phylogenetic tree of the isolated strains that resulted from the discovery of a new global strain of Proteus mirabilis bacteria and was recorded in NCBI genebank with accession number (MN 700085) and name (M.K.84 ).

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Identification of Proteus Mirabilis on Banknotes Using 16s rRNA gene in Khartoum State

Cited by 9 publications

References 37 publications

Down-Regulation of fliL Gene Expression by Ag Nanoparticles and TiO2 Nanoparticles in Pragmatic Clinical Isolates of Proteus mirabilis and Proteus vulgaris from Urinary Tract Infection

Down-Regulation of fliL Gene Expression by Ag Nanoparticles and TiO2 Nanoparticles in Pragmatic Clinical Isolates of Proteus mirabilis and Proteus vulgaris from Urinary Tract Infection

Identification of Novel Bacteria by Using 16S rRNA from Clinical and Soil Samples

Isolation and Molecular Identification of proteus mirabilis isolated from hospitals in the capital Baghdad

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