Identification of PSE and OXA beta-lactamase genes in Pseudomonas aeruginosa using PCR-restriction fragment length polymorphism

Bert, Frédéric; Branger, Catherine; Lambert-Zechovsky, N

doi:10.1093/jac/dkf069

Cited by 151 publications

(84 citation statements)

References 28 publications

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“…The most common imported ␤-lactamases found among P. aeruginosa isolates are penicillinases belonging to the molecular class A serine ␤-lactamases (PSE, CARB, and TEM families). Within this group, enzymes belonging to the PSE family appear to be the most prevalent (14). The therapeutic impact of these penicillinases is relatively limited since they do not impact the clinical efficacy of extended-spectrum cephalosporins, monobactams, or carbapenems.…”

Section: Imported Resistance Mechanismsmentioning

confidence: 99%

Antibacterial-ResistantPseudomonas aeruginosa: Clinical Impact and Complex Regulation of Chromosomally Encoded Resistance Mechanisms

2009

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SUMMARY Treatment of infectious diseases becomes more challenging with each passing year. This is especially true for infections caused by the opportunistic pathogen Pseudomonas aeruginosa, with its ability to rapidly develop resistance to multiple classes of antibiotics. Although the import of resistance mechanisms on mobile genetic elements is always a concern, the most difficult challenge we face with P. aeruginosa is its ability to rapidly develop resistance during the course of treating an infection. The chromosomally encoded AmpC cephalosporinase, the outer membrane porin OprD, and the multidrug efflux pumps are particularly relevant to this therapeutic challenge. The discussion presented in this review highlights the clinical significance of these chromosomally encoded resistance mechanisms, as well as the complex mechanisms/pathways by which P. aeruginosa regulates their expression. Although a great deal of knowledge has been gained toward understanding the regulation of AmpC, OprD, and efflux pumps in P. aeruginosa, it is clear that we have much to learn about how this resourceful pathogen coregulates different resistance mechanisms to overcome the antibacterial challenges it faces.

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Section: Imported Resistance Mechanismsmentioning

confidence: 99%

Antibacterial-ResistantPseudomonas aeruginosa: Clinical Impact and Complex Regulation of Chromosomally Encoded Resistance Mechanisms

2009

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“…The TEM-type enzymes described in P. aeruginosa, namely, TEM-4, TEM-21, TEM-24, and TEM-42, have been reported in rare isolates from France (3,13,25,30,42). A French survey indicated that only 10% of ticarcillin-resistant P. aeruginosa isolates (i.e., 1.9% of P. aeruginosa isolates) produce a TEMtype ␤-lactamase, whereas other narrow-spectrum ␤-lactamases (OXA and CARB) are more frequently encountered in that species (3).…”

Section: Epidemiologymentioning

confidence: 99%

Ambler Class A Extended-Spectrum β-Lactamases in Pseudomonas aeruginosa : Novel Developments and Clinical Impact

Weldhagen

Poirel

Nordmann

2003

Antimicrob Agents Chemother

210

155

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“…The presence of OXA-type enzymes in Enterobacteriaceae has been described infrequently. OXA-1, OXA-2, OXA-10, and OXA-17 were previously reported in some parts of the world [21].…”

Section: Iso-electric Focusingmentioning

confidence: 58%

First Report of OXA-4, an ESBL Isolated from Pseudomonas aeruginosa a South Indian Strain

Kingsley¹,

Verghese²

2012

Indian J Microbiol

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The OXA-type b-lactamases are so named because of their oxacillin-hydrolyzing abilities. In this study we characterize an extended spectrum b-lactamase, designated OXA-4, produced by a clinical isolate of Pseudomonas aeruginosa. ESBL production was detected by double disk synergy test. The P. aeruginosa isolate was obtained from endotracheal suction tip of 84 years old male patient diagnosed with CVA and hypertension. ESBL producing OXA b-lactamases was detected by PCR with primers specific to the conserved regions of the coding genes. Iso electric focusing was done to confirm the significance, sequencing the amplified product was also done. In the phenotypic identification, the strain was highly resistant to third generation cephalosporins and also to imipenem. The PCR amplified product for OXA b-lactamase was viewed at 919 bp. The pI point for the same was identified at 7.2. With the help of sequencing the amplified OXA b-lactamase was identified as OXA-4 gene. Here we report P. aeruginosa producing OXA-4 ESBL for the first time in the Indian subcontinent.

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Identification of PSE and OXA beta-lactamase genes in Pseudomonas aeruginosa using PCR-restriction fragment length polymorphism

Abstract: This approach is effective for screening P. aeruginosa for beta-lactamase gene carriage in epidemiological studies and for detecting new variants.

Cited by 151 publications

References 28 publications

Antibacterial-ResistantPseudomonas aeruginosa: Clinical Impact and Complex Regulation of Chromosomally Encoded Resistance Mechanisms

Antibacterial-ResistantPseudomonas aeruginosa: Clinical Impact and Complex Regulation of Chromosomally Encoded Resistance Mechanisms

Ambler Class A Extended-Spectrum β-Lactamases in Pseudomonas aeruginosa : Novel Developments and Clinical Impact

First Report of OXA-4, an ESBL Isolated from Pseudomonas aeruginosa a South Indian Strain

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