Identification of Putative Aquaporin Genes and Their Expression Analysis under Hypoxic Conditions in Soybean [<i>Glycine max</i>(L.) Merr.]

Matsuo, Naoki; Nanjo, Yohei; Tougou, Makoto; Nakamura, Takuji; Nishizawa, Keito; Komatsu, Setsuko; Shimamura, Satoshi

doi:10.1626/pps.15.278

Cited by 7 publications

(4 citation statements)

References 48 publications

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“…In radish, water absorption depends mostly on the water channels of the root tissue cells [39]. Furthermore, in soybean roots, the production of an aquaporin, which is a plasma membrane intrinsic protein, varies under hypoxic conditions [40]. Consequently, the changes in the abundance of aquaporin-like proteins in response to hypoxia were studied for each cultivar.…”

Section: Root Hydraulic Conductivity Fluctuates With Changes To Leaf mentioning

confidence: 99%

“…Thus, responses to hypoxia depend on the regulation of PIP1s, possibly by different mechanisms among cultivars. Matsuo et al de-scribed a relationship between hypoxia and changes to mRNA levels for the "Ere" soybean root aquaporin PIP2 gene GmPIP2; 2 [40]. The expression of this gene was downregulated in roots after a 3-h exposure to hypoxic conditions.…”

Section: Cultivar-specific Changes To the Plasma Membrane Intrinsic Pmentioning

confidence: 99%

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Hypoxia-Responsive Root Hydraulic Conductivity Influences Soybean Cultivar-Specific Waterlogging Tolerance

Jitsuyama¹

2017

AJPS

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Excess soil moisture induces hypoxic conditions and causes waterlogging injury in soybean [Glycine max (L.) Merr.]. This study investigated the mechanism underlying the development of waterlogging injury. Nine Japanese soybean cultivars with varying degrees of waterlogging tolerance were grown in a hydroponic system for 14 days under hypoxic conditions. Shoot and root biomasses and root hydraulic conductivity were measured at an early vegetative stage for plants under control and hypoxic conditions. Root morphological traits and intramembrane aquaporin proteins were also analyzed. The tolerance of each cultivar to field waterlogging was based on biomass changes induced by the hypoxia treatment. Root hydraulic conductivity responses to hypoxia were associated with changes in total dry weight, leaf dry weight, and leaf area. The effects of hypoxic conditions on root hydraulic conductivity were also represented by the changes in root morphology, such as total root length, thick-root length, and number of root tips. Additionally, a 32.3 kDa aquaporin-like protein seemed to regulate root hydraulic conductivity. Our results from a hydroponic culture suggest that the soybean cultivar-specific responses to hypoxic conditions in the rhizosphere reflect fluctuations in hydraulic conductivity related to root morphological or qualitative changes.

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Section: Root Hydraulic Conductivity Fluctuates With Changes To Leaf mentioning

confidence: 99%

Section: Cultivar-specific Changes To the Plasma Membrane Intrinsic Pmentioning

confidence: 99%

Hypoxia-Responsive Root Hydraulic Conductivity Influences Soybean Cultivar-Specific Waterlogging Tolerance

Jitsuyama¹

2017

AJPS

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“…Additionally, the ready accessibility of omics resources has improved our comprehension of the AQP transport mechanism in plants. For instance, genome-wide identification of AQP genes has been performed in numerous plant species, including rice (Oryza sativa), soybean (Glycine max L.), and bottle gourds (Lagenaria siceraria) [23][24][25], and these studies have shown that AQP genes are conserved across plant species. However, AQPs remain poorly understood in many species.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of Aquaporin Genes in Adzuki Bean (Vigna angularis) and Expression Analysis under Drought Stress

Tayade

Rana

Shafiqul

et al. 2022

IJMS

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The adzuki bean Vigna angularis (Wild.) is an important leguminous crop cultivated mainly for food purposes in Asian countries; it represents a source of carbohydrates, digestible proteins, minerals, and vitamins. Aquaporins (AQPs) are crucial membrane proteins involved in the transmembrane diffusion of water and small solutes in all living organisms, including plants. In this study, we used the whole genome sequence of the adzuki bean for in silico analysis to comprehensively identify 40 Vigna angularis aquaporin (VaAQP) genes and reveal how these plants react to drought stress. VaAQPs were compared with AQPs from other closely-related leguminous plants, and the results showed that mustard (Brassica rapa) (59), barrel medic (Medicago truncatula) (46), soybean (Glycine max) (66), and common bean (Phaseolus vulgaris L.) (41) had more AQP genes. Phylogenetic analysis revealed that forty VaAQPs belong to five subfamilies, with the VaPIPs (fifteen) subfamily the largest, followed by the VaNIPs (ten), VaTIPs (ten), VaSIPs (three), and VaXIPs (two) subfamilies. Furthermore, all AQP subcellular locations were found at the plasma membrane, and intron–exon analysis revealed a relationship between the intron number and gene expression, duplication, evolution, and diversity. Among the six motifs identified, motifs one, two, five, and six were prevalent in VaTIP, VaNIP, VaPIP, and VaXIP, while motifs one, three, and four were not observed in VaPIP1-3 and VaPIP1-4. Under drought stress, two of the VaAQPs (VaPIP2-1 and VaPIP2-5) showed significantly higher expression in the root tissue while the other two genes (VaPIP1-1 and VaPIP1-7) displayed variable expression in leaf tissue. This finding revealed that the selected VaAQPs might have unique molecular functions linked with the uptake of water under drought stress or in the exertion of osmoregulation to transport particular substrates rather than water to protect plants from drought. This study presents the first thorough investigation of VaAQPs in adzuki beans, and it reveals the transport mechanisms and related physiological processes that may be utilized for the development of drought-tolerant adzuki bean cultivars.

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“…Numerous recent studies have investigated the association between water relations and gene expression and/or protein levels of AQPs under various environmental conditions and in a range of plant species, providing information that may open the door to manipulating AQP expression to alter plant water-use efficiency ( Moshelion et al, 2015 ). While AQP genes have been characterized in several plant species using genome-wide analyses ( Matsuo et al, 2012 ; Deshmukh et al, 2015 ), information on AQPs in sorghum [ Sorghum bicolor (L.) Moench] is sparse, particularly as related to waterlogging stress.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Regulation of Aquaporin Genes of Sorghum [Sorghum bicolor (L.) Moench] in Response to Waterlogging Stress

et al. 2017

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Waterlogging is a significant environmental constraint to crop production, and a better understanding of plant responses is critical for the improvement of crop tolerance to waterlogged soils. Aquaporins (AQPs) are a class of channel-forming proteins that play an important role in water transport in plants. This study aimed to examine the regulation of AQP genes under waterlogging stress and to characterize the genetic variability of AQP genes in sorghum (Sorghum bicolor). Transcriptional profiling of AQP genes in response to waterlogging stress in nodal root tips and nodal root basal regions of two tolerant and two sensitive sorghum genotypes at 18 and 96 h after waterlogging stress imposition revealed significant gene-specific pattern with regard to genotype, root tissue sample, and time point. For some tissue sample and time point combinations, PIP2-6, PIP2-7, TIP2-2, TIP4-4, and TIP5-1 expression was differentially regulated in tolerant compared to sensitive genotypes. The differential response of these AQP genes suggests that they may play a tissue specific role in mitigating waterlogging stress. Genetic analysis of sorghum revealed that AQP genes were clustered into the same four subfamilies as in maize (Zea mays) and rice (Oryza sativa) and that residues determining the AQP channel specificity were largely conserved across species. Single nucleotide polymorphism (SNP) data from 50 sorghum accessions were used to build an AQP gene-based phylogeny of the haplotypes. Phylogenetic analysis based on single nucleotide polymorphisms of sorghum AQP genes placed the tolerant and sensitive genotypes used for the expression study in distinct groups. Expression analyses suggested that selected AQPs may play a pivotal role in sorghum tolerance to water logging stress. Further experimentation is needed to verify their role and to leverage phylogenetic analyses and AQP expression data to improve waterlogging tolerance in sorghum.

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Identification of Putative Aquaporin Genes and Their Expression Analysis under Hypoxic Conditions in Soybean [Glycine max(L.) Merr.]

Cited by 7 publications

References 48 publications

Hypoxia-Responsive Root Hydraulic Conductivity Influences Soybean Cultivar-Specific Waterlogging Tolerance

Hypoxia-Responsive Root Hydraulic Conductivity Influences Soybean Cultivar-Specific Waterlogging Tolerance

Genome-Wide Identification of Aquaporin Genes in Adzuki Bean (Vigna angularis) and Expression Analysis under Drought Stress

Characterization and Regulation of Aquaporin Genes of Sorghum [Sorghum bicolor (L.) Moench] in Response to Waterlogging Stress

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