Identification of putative ecdysteroid and juvenile hormone pathway genes in the shrimp Neocaridina denticulata

Sin, Simon Yung Wa; Kenny, Nathan J.; Qu, Zhe; Chan, Ka Wo; Chan, Katie W.S.; Cheong, Sam P. S.; Leung, Ricky Wai Tak; Chan, Ting Fung; Bendena, William G.; Chu, Ka Hou; Tobe, Stephen S.; Hui, Jerome H. L.

doi:10.1016/j.ygcen.2014.07.018

Cited by 81 publications

(52 citation statements)

References 82 publications

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“…However, in crustaceans, JHAMT has only been reported in the water flea D. pulex (Hui et al, 2010), D. magna and the shrimp N. denticulata (Sin et al, 2015). This study cloned and characterized a full-length cDNA encoding JHAMT in the swimming crab P. trituberculatus, which suggests that JHAMT may be more widely presented in crustaceans and play an important role in the biosynthesis of MF in crustaceans.…”

Section: Discussionmentioning

confidence: 91%

“…Recently, orthologs of JHAMT have been identified in three crustacean species, including the water flea Daphnia pulex (Hui et al, 2010), Daphnia magna (NCBI protein id: JAN67028.1) and the shrimp Neocaridina denticulata (Sin et al, 2015), which altered our earlier opinion that JHAMT is unique to insect. Presence of JHAMT in crustaceans raises the question about the potential interaction with MF biosynthesis and crustacean endocrinology.…”

Section: Introductionmentioning

confidence: 96%

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The potential role of juvenile hormone acid methyltransferase in methyl farnesoate (MF) biosynthesis in the swimming crab, Portunus trituberculatus

Xie

Tian

Liu

et al. 2016

Animal Reproduction Science

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Juvenile hormone (JH) and methyl farnesoate (MF) play essential roles in the development and reproduction of insects and crustaceans respectively. Juvenile hormone acid methyltransferase (JHAMT) catalyzes the methyl esterification in insect JH biosynthesis, while the corresponding step in crustacean MF biosynthesis was long thought to be catalyzed by farnesoic acid O-methyltransferase (FAMeT). However, the new discovery of JHAMT orthologs in crustaceans indicates that JHAMT may also play essential role in the MF biosynthesis in crustaceans. Here we cloned and characterized the full-length cDNA encoding JHAMT in the swimming crab Portunus trituberculatus (PtJHAMT). Sequence and structure analysis of PtJHAMT revealed that it was composed of a 6-stranded β sheet with 9 α helices, and contained a signature Sadenosyl-L-methionine (SAM) binding motif, which is the hallmark in all SAM dependent methyltransferases (SAM-MTs). Several active sites that are critical for the interaction of SAM and JH/FA substrate were also conserved in PtJHAMT. The gene expression of PtJHAMT was highly specific to the mandibular organ, which is the sole site of MF synthesis. PtJHAMT expression significantly increased in the late-vitellogenic stage and mature stage, which suggests a possible role of PtJHAMT in modulating ovarian development. The role of PtJHAMT and PtFAMeT in MF biosynthesis was further investigated by RNA interfering (RNAi). Injection of PtJHAMT and PtFAMeT dsRNA both led to a decrease in hemolymph MF titers. Injection of PtHMGR dsRNA caused the decrease in PtJHAMT expression, but had no effect on mRNA level of PtFAMeT. Together these results suggested that JHAMT and FAMeT are both involved in the MF biosynthesis in crustaceans, while the JHAMT is highly specific to FA substrate, and FAMeT may have more catalytic functions.

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Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 96%

The potential role of juvenile hormone acid methyltransferase in methyl farnesoate (MF) biosynthesis in the swimming crab, Portunus trituberculatus

Xie

Tian

Liu

et al. 2016

Animal Reproduction Science

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“…A draft genome for Eriocheir sinensis , the Chinese mitten crab, was recently released (Song et al 2016), but this assembly represents about two-thirds of the estimated genome size and consists of over 17,500 scaffolds with an N50 length of 0.224 Mb. A preliminary genome of N. denticulata is available (Kenny et al 2014), and genes in the juvenoid and ecdysteroid hormone synthetic pathways were identified in the genome (Sin et al 2015). However, scaffold lengths are too few in number (∼200 with lengths exceeding 5 kb) and too short (e.g., N50 of 0.0004 Mb and a maximum contig length of 0.124 Mb) for genome-scale analysis.…”

Section: Future Directions and Recommendationsmentioning

confidence: 99%

“…Gene ontology (GO) and pathway analysis require manual curation and modification for pathways unique to decapods or arthropods in general, such as neuropeptide signaling and ecdysteroid and juvenoid hormone synthetic pathways (Mykles 2011; Covi et al 2012; Tom et al 2013; Sin et al 2015;Das et al 2016a). Preliminary comparative transcriptomic studies suggest numerous unannotated, novel genes.…”

Section: Future Directions and Recommendationsmentioning

confidence: 99%

Resources and Recommendations for Using Transcriptomics to Address Grand Challenges in Comparative Biology

Mykles

Burnett

Durica

et al. 2016

Integr. Comp. Biol.

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High-throughput RNA sequencing (RNA-seq) technology has become an important tool for studying physiological responses of organisms to changes in their environment. De novo assembly of RNA-seq data has allowed researchers to create a comprehensive catalog of genes expressed in a tissue and to quantify their expression without a complete genome sequence. The contributions from the “Tapping the Power of Crustacean Transcriptomics to Address Grand Challenges in Comparative Biology” symposium in this issue show the successes and limitations of using RNA-seq in the study of crustaceans. In conjunction with the symposium, the Animal Genome to Phenome Research Coordination Network collated comments from participants at the meeting regarding the challenges encountered when using transcriptomics in their research. Input came from novices and experts ranging from graduate students to principal investigators. Many were unaware of the bioinformatics analysis resources currently available on the CyVerse platform. Our analysis of community responses led to three recommendations for advancing the field: (1) integration of genomic and RNA-seq sequence assemblies for crustacean gene annotation and comparative expression; (2) development of methodologies for the functional analysis of genes; and (3) information and training exchange among laboratories for transmission of best practices. The field lacks the methods for manipulating tissue-specific gene expression. The decapod crustacean research community should consider the cherry shrimp, Neocaridina denticulata, as a decapod model for the application of transgenic tools for functional genomics. This would require a multi-investigator effort.

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“…Its expression reached the climax at the inter‐moult stage and returned to low levels at the pre‐moult stages. It is well known that the eyestalk is an important endocrine organ that secretes a variety of neuropeptides including the moult‐inhibiting hormone (MIH), controlling the onset of specific moulting stages (Chen, Watson, Chen, Liu, & Lee, ; Sin et al, ). Previous studies showed that MIH expression in the eyestalk was higher at the inter‐moult stage and its expression in the pre‐moult stage was rapidly decreasing (Liang et al, ; Shi, Li, Zhou, Wang, & Chan, ).…”

Section: Discussionmentioning

confidence: 99%

Potential involvement of a DMRT family member ( Mr‐Dsx ) in the regulation of sexual differentiation and moulting in the giant river prawn Macrobrachium rosenbergii

et al. 2019

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Macrobrachium rosenbergii is an important aquaculture species known as the king of freshwater prawn. Macrobrachium rosenbergii displays remarkable sexual dimorphism, yet our knowledge of the molecular mechanism underpinning sex determination and differentiation of M. rosenbergii is still fragmentary. In this study, through in silico prediction and experimental characterization, we identified a new Dmrt gene (Mr-Dsx) from M. rosenbergii. The cDNA of Mr-Dsx included a 115 bp 5′ untranslated region (UTR), a 2,568 bp open reading frame (ORF) and a 259 bp 3′ UTR. Mr-Dsx was expressed in a wide range of tissues, and showed dynamic expression changes at different moulting stages in the testis, androgenic gland (AG) and eyestalk. In response to unilateral eyestalk ablation (UEA), Mr-Dsx was significantly induced in the testis and AG. Gene knockdown of Mr-Dsx resulted in pronounced suppression of the insulin-like androgenic hormone (IAG) gene. The results suggest that Mr-Dsx is likely to be negatively regulated by the eyestalk neurohormones, and Mr-Dsx may participate in the transcriptional activation of IAG. The study characterized the potential roles of a newly identified Dmrt homologue from M. rosenbergii, and would contribute to a deeper understanding of the molecular regulatory network underlying sex differentiation and moulting of prawns. K E Y W O R D S crustacean, Dmrt, Macrobrachium rosenbergii, moulting, sexual differentiation

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Identification of putative ecdysteroid and juvenile hormone pathway genes in the shrimp Neocaridina denticulata

Cited by 81 publications

References 82 publications

The potential role of juvenile hormone acid methyltransferase in methyl farnesoate (MF) biosynthesis in the swimming crab, Portunus trituberculatus

The potential role of juvenile hormone acid methyltransferase in methyl farnesoate (MF) biosynthesis in the swimming crab, Portunus trituberculatus

Resources and Recommendations for Using Transcriptomics to Address Grand Challenges in Comparative Biology

Potential involvement of a DMRT family member ( Mr‐Dsx ) in the regulation of sexual differentiation and moulting in the giant river prawn Macrobrachium rosenbergii

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