Identification of putative olfactory genes in newly hatched larvae of a Coleopteran ectoparasitoid <i>Dastarcus helophoroides</i> (Fairmaire) (Coleoptera: Bothrideridae) by transcriptome analysis

Li, Xiaojuan; Fang, Jianmin; Liu, Hongjian; Guo, Wenlei; Yin, Huayang

doi:10.1111/1748-5967.12436

Cited by 5 publications

(9 citation statements)

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“…The number of olfactory proteins in E . signifier is considerably fewer than the 26 OBPs and 21 CSPs identified in the transcriptomes of H. armigera larval antennae and mouthparts (Chang et al, 2017 ); the 20 OBPs, 11 CSPs, 9 ORs, 11 IRs, 7 GRs, and 4 SNMPs in the transcriptomes of newly hatched Dastarcushelophoroides larvae (Li et al, 2020 ); and the 58 ORs, 20 GRs, and 21 IRs in the transcriptomes of the antennae of males and females and the head tissue of neonates of Cydia pomonella (Walker et al, 2016 ). This reflects the scarcity of olfactory proteins of the original Lepidoptera group in the NCBI database, such as Hepialidae, resulting in less annotation of olfactory proteins in E .…”

Section: Discussionmentioning

confidence: 99%

“…The phylogenetic trees were colored and arranged in FigTree (Version 1.4.2). The phylogenetic analyses of OBPs were based on Dastarcus helophoroides (Li et al, 2020 ), Chrysomya megacephala (Wang et al, 2015 ) , Plutella xylostella (Zhu et al, 2016 ), S. exigua (Liu et al, 2015 ; Llopis-Gimenez et al, 2020 ), Helicoverpa armigera (Chang et al, 2017 ), and E. signifier . The CSPs tree was based on D. helophoroides (Li et al, 2020 ), C. megacephala (Wang et al, 2015 ) , S. exigua (Llopis-Gimenez et al, 2020 ), H. armigera (Chang et al, 2017 ), and E. signifier .…”

Section: Methodsmentioning

confidence: 99%

“…The phylogenetic analyses of OBPs were based on Dastarcus helophoroides (Li et al, 2020 ), Chrysomya megacephala (Wang et al, 2015 ) , Plutella xylostella (Zhu et al, 2016 ), S. exigua (Liu et al, 2015 ; Llopis-Gimenez et al, 2020 ), Helicoverpa armigera (Chang et al, 2017 ), and E. signifier . The CSPs tree was based on D. helophoroides (Li et al, 2020 ), C. megacephala (Wang et al, 2015 ) , S. exigua (Llopis-Gimenez et al, 2020 ), H. armigera (Chang et al, 2017 ), and E. signifier . The gene name and the Genbank number of P. xylostella and H. armigera are listed in Supplementary Material 2 ; other genes sequences are available in the reference article.…”

Section: Methodsmentioning

confidence: 99%

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Olfactory Proteins and Their Expression Profiles in the Eucalyptus Pest Endoclita signifier Larvae

et al. 2021

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Endoclita signifier Walker (Lepidoptera: Hepialidae), a polyphagous insect, has become a new wood-boring pest in Eucalyptus plantations in southern China since 2007, which represents a typical example of native insect adaptation to an exotic host. After the third instar, larvae move from soil to standing trees and damage the plants with a wormhole. Although females disperse to lay eggs, larvae can accurately find eucalyptus in a mingled forest of eight species, which leads us to hypothesize that the larval olfactory system contributes to its host selection. Herein, we investigated the transcriptomes of the head and tegument of E. signifer larvae and explored the expression profiles of olfactory proteins. We identified 15 odorant-binding proteins (OBPs), including seven general OBPs (GOPBs), six chemosensory proteins (CSPs), two odorant receptors (ORs), one gustatory receptor (GR), 14 ionotropic receptors (IRs), and one sensory neuron membrane protein (SNMP). Expression profiles indicated that all olfactory proteins, except for EsigCSP1, were expressed in the head, and most were also detected in non-olfactory tissues, especially thorax tegument. Furthermore, EsigOBP2, EsigOBP8, EsigGOBP1, EsigGOBP2, EsigGOBP5, EsigCSP3, EsigCSP5, and EsigOR1 were expressed most strongly in the head; moreover, EsigCSP3 expressed abundantly in the head. EsigGR1 exhibited the highest expression among all tissues. Besides phylogenetic analysis shows that EsigGOBP7 probably is the pheromone-binding protein (PBP) of E. signifier. This study provides the molecular basis for future study of chemosensation in E. signifier larvae. EsigCSP3 and EsigGR1, which have unique expression patterns, might be factors that govern the host choice of larvae and worth further exploration.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Olfactory Proteins and Their Expression Profiles in the Eucalyptus Pest Endoclita signifier Larvae

et al. 2021

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“…Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae) is an important ectoparasitoid of Cerambycidae beetles that is widely used in their biological control 7–9 . Indoor mass‐reared Dastarcus helophoroides has been widely released into woodland and achieved significant results of controlling Anoplophora glabripennis (Motschulsky), Monochamus alternatus , Massicus raddei (Blessig), Batocera horsfieldi (Hope), and Apriona swainsoni (Hope) around China 10,11 .…”

Section: Introductionmentioning

confidence: 99%

“…5,6 Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae) is an important ectoparasitoid of Cerambycidae beetles that is widely used in their biological control. [7][8][9] Indoor mass-reared Dastarcus helophoroides has been widely released into woodland and achieved significant results of controlling Anoplophora glabripennis (Motschulsky), Monochamus alternatus, Massicus raddei (Blessig), Batocera horsfieldi (Hope), and Apriona swainsoni (Hope) around China. 10,11 By releasing the parasitoid, the highest and average parasitism rate of Dastarcus helophoroides on Monochamus alternatus were 92.6% and 88.6% in the first year, respectively, and the spread of pine wilt disease vectored by Monochamus alternatus had been successfully controlled.…”

Section: Introductionmentioning

confidence: 99%

Chromosome‐level genome assembly of Dastarcus helophoroides provides insights into CYP450 genes expression upon insecticide exposure

Zhang

Pei

Zhang

et al. 2022

Pest Management Science

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BACKGROUND: Dastarcus helophoroides is an important natural enemy of cerambycids, and is wildly used in biological control of pests. Nevertheless, the absence of complete genomic information limits the investigation of the underlying molecular mechanisms. Here, a chromosome-level of Dastarcus helophoroides genome is assembled using a combination strategy of Illumina, PacBio, 10x™ Genomics, and Hi-C. RESULTS: The final assembly is 609.09 Mb with contig N50, scaffold N50 and GC content of 5.46 Mb, 42.56 Mb and 31.50%, respectively, and 95.25% of the contigs anchor into 13 chromosomes. In total 14 890 protein-coding genes and 65.37% repeat sequences are predicted in the assembly genome. The phylogenetic analysis of single-copy gene families shared among 20 insect species indicates that Dastarcus helophoroides is placed as the sister species to clade (Nitidulidae+Curculionoidea +Chrysomeloidea) + Tenebrionoidea, and diverges from the related species ∼242.9 Mya. In total 36 expanded gene families are identified in Dastarcus helophoroides genome, and are functionally related to drug metabolism and metabolism of xenobiotics by cytochrome P450. Some members of CYP4 Clade and CYP6 Clade are up-regulated in Dastarcus helophoroides adults upon insecticide exposure, of which expressions of DhCYP4Q, DhCYP6A14X1 and DhCYP4C1 are significantly up-regulated. The silencing of the three genes leads to adults more sensitive to insecticide and increased knocked-down rate, which may indicate their critical roles in stress resistance and detoxication.CONCLUSION: Our study systematically integrated the chromosome-level genome, transcriptome and gene expression of Dastarcus helophoroides, which will provide valuable resources for understanding mechanisms of pesticide metabolism, growth and development, and utilization of the natural enemy in integrated control.

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Olfactory proteins of Endoclita signifer larvae and their roles in host recognition

Qiu

Chen

et al. 2022

Chem. Biol. Technol. Agric.

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Background Endoclita signifer causes severe damage to eucalyptus plantations, and the larvae transfer to and damage eucalyptus accurately in mixed forests, suggesting that the larval olfactory system contributes to host selection. The olfactory proteins in the head and tegument of E. signifer larvae were previously identified. To identify the relationship between olfactory protein expression in the larval head the larvae head and the developmental expression dynamics, and its functions in further recognition of plant volatiles, the head transcriptomes of two instar larvae and the expression profiles of olfactory proteins in the instars after exposure to volatiles were studied. Results Eight odorant-binding proteins, six chemosensory proteins, three odorant receptors, three gustatory receptors, and 18 ionotropic receptors were identified. Half of the olfactory proteins were the most highly expressed in the young (5th) larval head, and EsigGOBP2, EsigGOBP4, EsigGOBP5, EsigCSP1, EsigCSP3, EsigGR1 and EsigGR3 were highly expressed and showed a specific expression pattern. In addition, after exposure to o-cymene, α-phellandrene, n-butyl ether, and 4-ethylacetophenone, EsigGR3 was downregulated significantly, and exposure to n-butyl ether caused EsigGR1 to be downregulated significantly. Conclusions Seven specific olfactory proteins may be important genes in larval olfactory recognition. Furthermore, based on the receptors that were downregulated after exposure to volatiles and the previous electrophysiological activity in the third larvae, we speculated that the ligand of EsigGR1 was n-butyl ether, and the ligands of the newly identified EsigGR3 are all electrophysiologically active compounds, which demonstrated host recognition in the third larvae of E. signifer. These results provide a way to find key plant volatiles recognized by the key olfactory proteins as new targets for pest control. Graphical Abstract

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Identification of putative olfactory genes in newly hatched larvae of a Coleopteran ectoparasitoid Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae) by transcriptome analysis

Cited by 5 publications

References 43 publications

Olfactory Proteins and Their Expression Profiles in the Eucalyptus Pest Endoclita signifier Larvae

Olfactory Proteins and Their Expression Profiles in the Eucalyptus Pest Endoclita signifier Larvae

Chromosome‐level genome assembly of Dastarcus helophoroides provides insights into CYP450 genes expression upon insecticide exposure

Olfactory proteins of Endoclita signifer larvae and their roles in host recognition

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