Identification of pyrene degraders via DNA-SIP in oilfield soil during natural attenuation, bioaugmentation and biostimulation

Teng, Tingting; Ji, Liang; Wu, Zijun

doi:10.1016/j.scitotenv.2021.149485

Cited by 21 publications

(5 citation statements)

References 56 publications

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“…A previous study from our lab has also shown that Azospirillum has the potential for the degradation of hydrocarbon [67]. Another essential genus found in these soils, Skermanella sp., was reported as a pyrene degrader in a study involving an oilfield soil that used natural attenuation, bioaugmentation, and bio-stimulation approaches in the degradation of pyrene [6]. Thus, their presence in the studied soil could be associated with their roles as bio-degraders in crude oil-contaminated soil.…”

Section: Discussionmentioning

confidence: 89%

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Assessment of the Microbial Communities in Soil Contaminated with Petroleum Using Next-Generation Sequencing Tools

García-García¹,

Bocanegra-García

Vital-López

et al. 2023

Applied Sciences

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Microbial communities are known to play a principal role in petroleum degradation. This study tries to determine the composition of bacteria in selected crude oil-contaminated soil from Tabasco and Tamaulipas states, Mexico. We determined the microbial populations living under these conditions. We evaluated the structure and diversity of bacterial communities in the contaminated soil samples. The most abundant phylum is proteobacteria. Next Generation Sequencing (NGS) analysis of the sampled soils from both states revealed that this phylum has the most relative abundance among the identified bacteria phyla. The heatmap represented the relative percentage of each genus within each sample and clustered the four samples into two groups. Moreover, this allowed us to identify many genera in alkaline soil from Tamaulipas, such as Skermanella sp., Azospirillum sp. and Unclassified species from the Rhodospirillaceae family in higher abundance. Meanwhile, in acidic soil from Tabasco, we identified Thalassospira, Unclassified members of the Sphingomonadaceae family and Unclassified members of the Alphaproteobacteria class with higher abundance. Alpha diversity analysis showed a low diversity (Shannon and Simpson index); Chao observed species in both Regions. These results suggest that the bacteria identified in these genera may possess the ability to degrade petroleum, and further studies in the future should elucidate their role in petroleum degradation.

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Section: Discussionmentioning

confidence: 89%

“…2023, 13, 6922 2 of 16 of the soil often comes from activities such as oil exploitation, smelting, oil transportation, etc. [6]. Among the largest producers of petroleum worldwide are the United States, Canada, Brazil, and Mexico.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Microbial Communities in Soil Contaminated with Petroleum Using Next-Generation Sequencing Tools

García-García¹,

Bocanegra-García

Vital-López

et al. 2023

Applied Sciences

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“…Finally, a low number of ASVs were affiliated to Perlucidibaca . This genus was described by Teng and colleagues [ 41 ] as responsible for the degradation of pyrene in oil land soils. The ability to degrade complex hydrocarbons is certainly a powerful function in POME-fertilized soil.…”

Section: Discussionmentioning

confidence: 99%

Soil Fertilization with Palm Oil Mill Effluent Has a Short-Term Effect on the Bacterial Diversity of an Amazonian Agricultural Land Area

Sanches,

Costa,

das Graças

et al. 2024

Microorganisms

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Palm oil derived from the fruits of Elaeis guineensis Jacq. has global economic importance and is largely produced in tropical regions. The palm oil production process leads to a highly polluting waste called palm oil mill effluent (POME). A strategy commonly used by producers to overcome environmental issues and to improve soil fertility is the reuse of POME as a fertilizer due to the chemical and biological characteristics of the effluent. In this research, three groups were analyzed: soil without POME application (control group) and soil samples after 4 and 9 days of POME application. An environmental DNA metabarcoding approach was used. eDNA was extracted, and the V4 region of the 16S rRNA gene was amplified and sequenced in the Illumina MiSeq platform. The abundance of Proteobacteria (48.1%) and Firmicutes (9.0%) was higher in fertilized soil, while Bacteroidetes (20.3%) and Verrucomicrobia (7.8%) were more abundant in control soil. Additionally, the effluent seemed to modify soil characteristics favoring taxa responsible for the mineralization of organic compounds and nitrogen fixation such as species of Gammaproteobacteria class. Our study highlights the influence of POME on soil biological components and contributes to the sustainable production of palm oil in the Amazon.

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“…HTS can be used with DNA-SIP to study intricate background ecosystems ( Hatzenpichler et al, 2020 ). HTS-DNA-SIP has made it possible to determine isotope incorporation patterns in several microbial species ( Teng et al, 2021 ). The fundamental benefit of SIP is the ability to examine different environmental samples, such as water, soil, and sediment, without the need to first identify the organisms involved in the biodegradation of the contaminants.…”

Section: High Throughput Sequencing Technologymentioning

confidence: 99%

Cutting edge tools in the field of soil microbiology

Garg,

Patel,

Rawat

et al. 2024

Current Research in Microbial Sciences

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Identification of pyrene degraders via DNA-SIP in oilfield soil during natural attenuation, bioaugmentation and biostimulation

Cited by 21 publications

References 56 publications

Assessment of the Microbial Communities in Soil Contaminated with Petroleum Using Next-Generation Sequencing Tools

Assessment of the Microbial Communities in Soil Contaminated with Petroleum Using Next-Generation Sequencing Tools

Soil Fertilization with Palm Oil Mill Effluent Has a Short-Term Effect on the Bacterial Diversity of an Amazonian Agricultural Land Area

Cutting edge tools in the field of soil microbiology

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