Identification of QTLs and critical genes related to sugarcane mosaic disease resistance

Abstract

Mosaic viral diseases affect sugarcane productivity worldwide. Mining disease resistance-associated molecular markers or genes is a key component of disease resistance breeding programs. In the present study, 285 F1 progeny were produced from a cross between Yuetang 93-159, a moderately resistant variety, and ROC22, a highly susceptible variety. The mosaic disease symptoms of these progenies, with ROC22 as the control, were surveyed by natural infection under 11 different environmental conditions in the field and by artificial infections with a mixed sugarcane mosaic virus (SCMV) and sorghum mosaic virus (SrMV) inoculum. Analysis of consolidated survey data enabled the identification of 29 immune, 55 highly resistant, 70 moderately resistant, 62 susceptible, and 40 highly susceptible progenies. The disease response data and a high-quality SNP genetic map were used in quantitative trait locus (QTL) mapping. The results showed that the correlation coefficients (0.26~0.91) between mosaic disease resistance and test environments were significant (p< 0.001), and that mosaic disease resistance was a highly heritable quantitative trait (H2 = 0.85). Seven mosaic resistance QTLs were located to the SNP genetic map, each QTL accounted for 3.57% ~ 17.10% of the phenotypic variation explained (PVE). Furthermore, 110 pathogen response genes and 69 transcription factors were identified in the QTLs interval. The expression levels of nine genes (Soffic.07G0015370-1P, Soffic.09G0015410-2T, Soffic.09G0016460-1T, Soffic.09G0016460-1P, Soffic.09G0017080-3C, Soffic.09G0018730-3P, Soffic.09G0018730-3C, Soffic.09G0019920-3C and Soffic.03G0019710-2C) were significantly different between resistant and susceptible progenies, indicating their key roles in sugarcane resistance to SCMV and SrMV infection. The seven QTLs and nine genes can provide a certain scientific reference to help sugarcane breeders develop varieties resistant to mosaic diseases.

Sugarcane breeding: a fantastic past and promising future driven by technology and methods

“…However, so far, only the closely linked markers R12H16 and 9O20-F4 for resistance to the brown rust Bru1 gene (Le Cunff et al, 2008) and the G1 marker for resistance to orange rust (Yang et al, 2018) have been used in sugarcane breeding. Recently, single nucleotide polymorphism (SNP) microarrays established on high-throughput sequencing began to be used for target trait localization, and quantitative trait locus (QTLs) related to yellow leaf (You et al, 2019), ratoon stunting (You et al, 2021), leaf blight (Wang et al, 2022a), and mosaic disease (Lu et al, 2023) resistance, as well as chlorophyll content History and future perspective of sugarcane breeding. (Lu et al, 2021), tillering and ratooning ability (Wang et al, 2023a) were obtained, nevertheless none of them have achieved subsequent progress in application.…”

Sustentabilidade em Foco: Abordagens Interdisciplinares em Agropecuária e Meio Ambiente

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Influence of IBA Concentrations on the Success and Growth Characteristics of Grape Rooting Cuttings (Vitis vinifera L.)

“…Grapes were domesticated between 6,000-8,000 years ago in the region between the Black and Caspian Seas. They subsequently spread east into Asia and west into the Mediterranean region (Lu, et al, 2023).…”