Identification of QTLs for powdery mildew and scald resistance in barley

Abstract

A population of 103 recombinant inbred lines (RILs, F 9 -derived lines) developed from the tworow spring barley cross L94×'Vada' was evaluated under field conditions for resistance against powdery mildew (Blumeria graminis f.sp. hordei) and scald (Rhynchosporium secalis). Apart from the major resistance gene mlo on chromosome 4 (4H), three QTLs (Rbgq1, Rbgq2 and Rbgq3) for resistance against powdery mildew were detected on chromosomes 2 (2H), 3 (3H), and 7 (5H), respectively. Rbgq1 and Rbgq2 have not been reported before, and did not map to a chromosome region where a major gene for powdery mildew had been reported. Four QTLs (Rrsq1, Rrsq2, Rrsq3 and Rrsq4) for resistance against scald were detected on chromosomes 3 (3H), 4 (4H) and 6 (6H). All four mapped to places where QTLs for scald resistance had been reported before in different populations.

Combination of seedling and adult plant resistance to leaf scald for stable resistance in barley

“…QSc.VlWa.6H was located on the short arm of chromosome 6H. Previous reports identified other QTLs for scald resistance in a similar region (Cheong et al 2006;Gronnerod et al 2002;Jensen et al 2002;Shtaya et al 2006;Spaner et al 1998). This suggests that QSc.VlWa.6H might be the same as QTLs reported in other genetic materials.…”

“…In total, 43QTLs and seven genes conferring quantitative and qualitative resistance to R. secalis have been located on individual maps (Abbott et al 1992(Abbott et al , 1995Backes et al 1995;Bjornstad et al , 2004Cheong et al 2006;Garvin et al 1997Garvin et al , 2000Genger et al 2003aGenger et al , b, 2005Gronnerod et al 2002;Jensen et al 2002;Li and Zhou 2011;Patil et al 2003;Pickering et al 2006;Sayed et al 2004;Schweizer et al 1995Schweizer et al , 2004Shtaya et al 2006;Spaner et al 1998;von Korff et al 2005;Wagner et al 2008;Yun et al 2005). Here, we integrated all these QTLs and genes into a consensus map to assess the genetic variation of scald resistance (Fig.…”

Basal Host Resistance of Barley to Powdery Mildew: Connecting Quantitative Trait Loci and Candidate Genes

“…Similarly, QTL analyses for FHB resistance in different barley mapping populations indicated that the quantitative resistance of barley to FHB is conditioned by many loci (Mesfin et al 2003). QTL for powdery mildew resistance have previously been reported in different mapping populations of barley (Heun 1992;Backes et al 1995Backes et al , 1996Backes et al , 2003Spaner et al 1998;Falak et al 1999;Read et al 2003;Von Korff et al 2005;Shtaya et al 2006). We compared the position of the peak marker of the presently found QTL with the peak mark- ers of 28 reported QTL using our high-density integrated map of barley.…”

“…Fifteen candidate gene sequences remained for which no polymorphism was detected or for which it was not possible to obtain a clear polymerase chain reaction (PCR) amplification product in any of the parental combinations (clone ID: HO14H18, HO14K08, HO16C19, HO26F22, HO13M13, HZ40O15, HI05K17, HO15M05, HO13K06, HW03O11, HU02G09, AJ344223, AJ518932, AJ518933, and AM265370). The position of the mlo gene was determined directly in the LnVa map as being on chromosome 4H (Qi et al 1998;Shtaya et al 2006). Ror1 is located within a 0.1 cM distance of the ABG452 marker on chromosome 1H (Collins et al 2001).…”

“…Because L94 is an mlo-barley, it is fully resistant to powdery mildew. Thus, in our inoculation experiments, we only included non-mlo-carrying RIL (51 accessions) of the LnVa population (Shtaya et al 2006).…”