Identification of Quantitative Trait Loci Controlling Symptom Development During Viral Infection inArabidopsis thaliana

Abstract

In compatible interactions between plants and viruses that result in systemic infection, symptom development is a major phenotypic trait. However, host determinants governing this trait are mostly unknown, and the mechanisms underlying it are still poorly understood. In a previous study on the Arabidopsis thaliana-Plum pox virus (PPV) pathosystem, we showed a large degree of variation in symptom development among susceptible accessions. Two basic types of interaction exist between a virus and its host plant: i) a compatible interaction associated with plant invasion and viral multiplication, followed in most cases by the induction of symptoms, and ii) an incompatible interaction with no or only limited viral multiplication or movement in the host plant. Plant-virus interactions are relatively well documented, but efforts have so far focused on resistance (or resistancebreaking) mechanisms and on the identification of their genetic determinants. Conversely, the one or more mechanisms controlling symptom development in plants are still largely unknown, so that only a very patchy picture of the interaction between a plant and a virus can be presented.

Quantitative trait loci analysis of Plum pox virus resistance in Prunus davidiana P1908: new insights on the organization of genomic resistance regions

“…These findings suggest that this common region probably contributes to a major part of the PPV resistance in P. davidiana P1908 but seems to be more associated to virus accumulation and distribution in the plant than to symptom severity. This suggests that symptom severity and virus accumulation are associated with different genomic regions as reported in Sicard et al (2008) who detected QTL associated to symptom severity (SYMP) and virus accumulation (ACC) for PPV infection in A. thaliana. Besides this, these findings suggest that the major QTL in LG6 is probably the one which contributes the most to the reduction of symptom severity since a correlation seems to exist between the high level of symptom severity and the low effect of this QTL in the RD population.…”

“…Verticals bars on the right indicate the position and LOD-1 confidence interval for each QTL. The shaded areas in the linkage groups represent the QTL position and interval Arabidopsis thaliana Sicard et al 2008). It is essentially based on visual observation of symptoms in the plants followed by confirmation of the virus accumulation with ELISA or PCR techniques.…”

“…The implication of components of the eukaryotic translation initiation complex has been reported in several virus/plant pathosystems including A. thaliana (Maule et al 2007;Nicaise et al 2007), and among these ones the eukaryotic initiation factor 4E (eIF4E) has been identified as a recessive inherited resistance locus in pepper, lettuce, and pea against several potyvirus (Kang et al 2005). Decroocq et al (2005) and Sicard et al (2008) mapped several candidate genes involved in the eukaryotic translation initiation complex in peach and apricot and suggested the involvement of some of them in resistance to PPV. In addition, Marandel et al (2009) mapped SSRs linked to a component of the eukaryotic translation initiation complex (eIF(iso)4G) to the region of the major QTL in LG6, even if these authors did not demonstrate a link between the resistance and a specific allele.…”

A Plant Small Polypeptide Is a Novel Component of DNA-Binding Protein Phosphatase 1-Mediated Resistance to Plum pox virus in Arabidopsis

“…Besides the unquestionable key role of eIF4E and eIF(iso)4E, which are essential factors in potyvirus infection, this supports the notion that resistance to potyvirus is a multifaceted response resulting from a complex balance between the attempt of the virus to use plant components to its own benefit and the defense mechanisms of the plant, with multiple host factors being involved in such an interaction. In accordance with this idea, symptoms induced by PPV infection in Arabidopsis show high variability among different accessions and also within specific susceptible ecotypes such as Col-0 (Decroocq et al, 2006;Sicard et al, 2008). The differences in expression levels and/or in the regulation of protein functions involved may account, at least partly, for this variability in a quantitative manner.…”

Identificación y caracterización de la familia de factores DBP, nuevos reguladores de la expresión génica en plantas.

“…These results suggest -0 (20). In this interaction, correlation analysis showed weak linearity beween symptom severity and virus accumulation, which both seem to be polygenic and quantitative traits (21). We also analyzed the performance of atdbp1 plants against another potyvirus, Turnip Mosaic Virus (TuMV), which causes more severe symptoms in Col-0 than PPV infection.…”