Identification of Quantitative Trait Loci Associated With Partial Resistance to Fusarium Root Rot and Wilt Caused by Fusarium graminearum in Field Pea

Wu, Liren; Fredua-Agyeman, Rudolph; Strelkov, Stephen E.; Chang, K. F.; Hwang, Sheau‐Fang

doi:10.3389/fpls.2021.784593

Cited by 8 publications

(10 citation statements)

References 74 publications

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“…The results of the current study validated the stability of genetic resistance in ‘00-2067’. Similar to our previous studies [ 25 , 67 ], significant genotypic effects, a high correlation coefficient within each trait and a negative correlation of root rot severity with vigor and plant height were observed in the RIL population derived from ‘00-2067’ × ‘Carman’. The frequency distribution of the disease severity data for the RIL population suggested that the resistance in ‘00-2067’ was transferred to the progenies in the RIL lines.…”

Section: Discussionsupporting

confidence: 89%

“…The use of partially resistant cultivars is the most effective method to control this disease, particularly given the lack of fully resistant genotypes. The pea cultivar ‘00-2067’ was reported to be partially resistant to infection by A. euteiches under field conditions [ 22 ], and this partial resistance to ARR as well as to Fusarium root rot was further explored in recent studies [ 25 , 67 ]. The most stable and major QTL for resistance to the root rot complex were mapped to two genomic regions on chromosome 4, while minor to moderate QTL were located on chromosomes 5, 6 and 7 [ 19 , 62 ].…”

Section: Discussionmentioning

confidence: 99%

“…The QTL identified by Wu et al [ 25 , 67 ] were determined using an F 8 RIL population derived from the cross ‘00-2067’ (root rot-resistant parent) × ‘Reward’ (susceptible parent). To study the inheritance of the identified QTLs in a different genetic background, we crossed the ARR and Fusarium root rot resistant parent ‘00-2067’ with the susceptible cultivar ‘Carman’ and developed RIL of 135 individuals.…”

Section: Discussionmentioning

confidence: 99%

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Identification of Novel Genes Associated with Partial Resistance to Aphanomyces Root Rot in Field Pea by BSR-Seq Analysis

Wu¹,

Fredua-Agyeman²,

Strelkov³

et al. 2022

IJMS

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Aphanomyces root rot, caused by Aphanomyces euteiches, causes severe yield loss in field pea (Pisum sativum). The identification of a pea germplasm resistant to this disease is an important breeding objective. Polygenetic resistance has been reported in the field pea cultivar ‘00-2067’. To facilitate marker-assisted selection (MAS), bulked segregant RNA-seq (BSR-seq) analysis was conducted using an F8 RIL population derived from the cross of ‘Carman’ × ‘00-2067’. Root rot development was assessed under controlled conditions in replicated experiments. Resistant (R) and susceptible (S) bulks were constructed based on the root rot severity in a greenhouse study. The BSR-seq analysis of the R bulks generated 44,595,510~51,658,688 reads, of which the aligned sequences were linked to 44,757 genes in a reference genome. In total, 2356 differentially expressed genes were identified, of which 44 were used for gene annotation, including defense-related pathways (jasmonate, ethylene and salicylate) and the GO biological process. A total of 344.1 K SNPs were identified between the R and S bulks, of which 395 variants were located in 31 candidate genes. The identification of novel genes associated with partial resistance to Aphanomyces root rot in field pea by BSR-seq may facilitate efforts to improve management of this important disease.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Identification of Novel Genes Associated with Partial Resistance to Aphanomyces Root Rot in Field Pea by BSR-Seq Analysis

Wu¹,

Fredua-Agyeman²,

Strelkov³

et al. 2022

IJMS

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“…The disease can reduce the pea crop yield by 30% to 57% [17]. Pea root rot is sometimes referred to as Fusarium root rot as Fusarium species are the predominant pathogens [16,18]). Typical symptoms of root rot are dark-brown lesions on the roots (especially at the base of the roots, close to the soil level); dark spots on the cotyledons, epicotyl, or base of the shoot; and leaf yellowing [19].…”

Section: Introductionmentioning

confidence: 99%

Antifungal Properties of Bio-AgNPs against D. pinodes and F. avenaceum Infection of Pea (Pisum sativum L.) Seedlings

Stałanowska,

Szablińska-Piernik,

Pszczółkowska

et al. 2024

IJMS

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Ascochyta blight and Fusarium root rot are the most serious fungal diseases of pea, caused by D. pinodes and F. avenaceum, respectively. Due to the lack of fully resistant cultivars, we proposed the use of biologically synthesized silver nanoparticles (bio-AgNPs) as a novel protecting agent. In this study, we evaluated the antifungal properties and effectiveness of bio-AgNPs, in in vitro (poisoned food technique; resazurin assay) and in vivo (seedlings infection) experiments, against D. pinodes and F. avenaceum. Moreover, the effects of diseases on changes in the seedlings’ metabolic profiles were analyzed. The MIC for spores of both fungi was 125 mg/L, and bio-AgNPs at 200 mg/L most effectively inhibited the mycelium growth of D. pinodes and F. avenaceum (by 45 and 26%, respectively, measured on the 14th day of incubation). The treatment of seedlings with bio-AgNPs or fungicides before inoculation prevented the development of infection. Bio-AgNPs at concentrations of 200 mg/L for D. pinodes and 100 mg/L for F. avenaceum effectively inhibited infections’ spread. The comparison of changes in polar metabolites’ profiles revealed disturbances in carbon and nitrogen metabolism in pea seedlings by both pathogenic fungi. The involvement of bio-AgNPs in the mobilization of plant metabolism in response to fungal infection is also discussed.

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“…2012; Wu et al. 2022), it is not clear how different pea accessions and varieties with pigmented or nonpigmented flowers will respond to a highly aggressive F. avenaceum isolate.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Pea Accessions Differing in Flower and Seed Coat Pigmentation for Resistance to Fusarium avenaceum Root Rot

Awodele,

Gali,

Sivachandra Kumar

et al. 2024

Legume Science

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Pea production across the world is significantly limited by root rot disease, which is caused by many fungal and oomycetes pathogens. In Canada, Fusarium avenaceum is the most devastating pathogen of the Fusarium root rot complex of pea. Host genetic resistance is the most effective control method for this disease. Evaluation of global pea accessions and Canadian varieties for F. avenaceum root rot resistance has not been reported to date. This study evaluated 20 pea accessions of different market classes with pigmented or nonpigmented seed coats and flowers for F. avenaceum resistance under controlled conditions. The pea accessions CDC Acer, CDC Vienna, PBA OURA, Morgan, CDC Blazer, CDC Dakota, and PI 280609, which have pigmented flowers and seed coats, were identified as resistant or partially resistant to F. avenaceum. This was based on their root rot severity scores and ability to tolerate F. avenaceum infection without significant (p > 0.05) reductions in plant height, shoot dry weight, and root dry weight. Among the varieties with nonpigmented flowers and seed coats, only Cameor showed partial resistance to F. avenaceum when challenged with reduced conidial concentration. Root dry weight (R = −0.86), plant height (R = −0.82), and shoot dry weight (R = −0.78) had a strong negative correlation (p < 0.001) with disease severity, suggesting that F. avenaceum root rot can negatively impact the growth and development of pea seedlings. F. avenaceum resistance identified in this study can be utilized to study the molecular basis of the resistance and develop disease‐resistant varieties. While our findings suggest a relationship between pigmentation and F. avenaceum resistance, future research with a larger, more diverse panel is warranted to validate these initial results.

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Identification of Quantitative Trait Loci Associated With Partial Resistance to Fusarium Root Rot and Wilt Caused by Fusarium graminearum in Field Pea

Cited by 8 publications

References 74 publications

Identification of Novel Genes Associated with Partial Resistance to Aphanomyces Root Rot in Field Pea by BSR-Seq Analysis

Identification of Novel Genes Associated with Partial Resistance to Aphanomyces Root Rot in Field Pea by BSR-Seq Analysis

Antifungal Properties of Bio-AgNPs against D. pinodes and F. avenaceum Infection of Pea (Pisum sativum L.) Seedlings

Evaluation of Pea Accessions Differing in Flower and Seed Coat Pigmentation for Resistance to Fusarium avenaceum Root Rot

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