Identification of quantitative trait loci underlying seed oil content of soybean including main, epistatic and QTL×environment effects in different regions of Northeast China

Abstract

Oil content is a primary trait in soybean and determines the quality of soy food, feed and oil product. Increasing oil content is a major objective of soybean breeding. The aims of the present study were to identify quantitative trait loci (QTLs) and epistatic QTLs associated with oil content in soybean seed by using 129 recombinant inbred lines derived from a cross between cultivar Dongnong 46 (oil content 22.53%) and the semi-wild line L-100 (oil content 17.33%). Phenotypic data were collected from 10 tested environments including Harbin in the years 2012–15, Hulan in 2013–15 and Acheng in 2013–15. A genetic linkage map including 213 simple sequence repeat markers in 18 chromosomes (or linkage groups) was constructed, covering ~3623.39 cM. Seven QTLs, located on five chromosomes (or linkage groups), were identified to be associated with oil content, explaining 2.24–17.54% of the phenotypic variation in multi-environments. Among these identified QTLs, five (qOIL-2, qOIL-4, qOIL-5, qOIL-6 and qOIL-7) were detected in more than five environments. Seven QTLs had additive and/or additive × environment interaction effects. QTLs with higher additive effects were more stable in multi-environments than those with lower additive effects. Moreover, five epistatic, pairwise QTLs were identified in different environments. The findings with respect to genetic architecture for oil content could be valuable for marker-assisted selection in soybean breeding programs for high oil content.

Identification of additive and epistatic effects QTLs for seed oil content in soybean based on an integrating map of two RIL populations

“…In present study, PVE of A and AE of 64 additive QTL ranged from 1.29% to 10.75% and 0.86% to 10.66%, respectively, while those of AA and AAE effect of 12 pairs of epistatic QTL ranged from 1.61% to 3.31% (Figure 3 and Table 5). By the comparison, AA and AAE effect were less than A and AE effect, this is similar to the study of Teng et al (2017) [22]. Epistasis may also play an essential role in trait improvement even if epistatic variance components are low [17,54].…”

“…Epistasis, interaction between one pair of loci located in the same or different chromosomes, as an important genetic basis of complex traits [18]. Some studies had shown that epistasis signi cantly affected the expression of genes and genetic variation underlying soybean [17,[19][20][21][22][23][24]. Hou et al(2014) [20] mapped oil content QTLs using simple repeat sequence (SSR) markers derived from Charleston and Dongnong594, which detected 4 pairs oil epistatic effect QTLs, and Qi et al (2017) [12] identi ed epistatic interactions for seed oil content under multi-environments based on a high-density single-nucleotide polymorphisms (SNP) using the same population.…”

Nutritional Composition and Volatile Compounds of Black Cumin (Nigella sativa L.) Seed, Fatty Acid Composition and Tocopherols, Polyphenols, and Antioxidant Activity of Its Essential Oil

“…In a literature study, approximate moisture analysis of black cumin seed found that crude protein ranged from 20.6 to 31.2%, ash ranged from 3.9 to 4.8%, fats ranged from 22.6 to 53.4%, carbohydrates varied from 24.9 to 34%, and moisture ranged 3.8 to 7.4% [2,4,6,16,17]. The black seed had higher fat content than corn (10%) [18] and soybean (20%) [19], suggesting that the black seed would be a suitable source for oil industry applications. Additionally, the black seed is an excellent source of fats and proteins for humans.…”

Characterization of a novel squalene‐rich oil: Pachira macrocarpa seed oil

“…As shown in Table 1, the moisture, lipid, protein, and ash contents of P. macrocarpa seed were 6.23%, 43.34%, 15.21%, and 3.90%, respectively. The lipid content exceeded that of many common oil crops, such as soybean (∼20%) (Teng et al., 2017) and corn (∼11%) (Navarro et al., 2016), suggesting P. macrocarpa seed may be suitable for the oil industry application.…”