Identification of RANKL in Osteolytic Lesions of the Facial Skeleton

Tay, Jessica; Bay, Boon‐Huat; Yeo, Jaeseung; Harris, Malcolm; Meghji, S; Dheen, S. Thameem

doi:10.1177/154405910408300415

Cited by 63 publications

(52 citation statements)

References 28 publications

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“…RANKL promotes the formation of osteoclast by bonding with cytokine, which influences bone remodeling. OPG inhibits the formation of osteoclast by inhibiting RANKL [9].…”

Section: Introductionmentioning

confidence: 99%

Effect of low‐level laser treatment after installation of dental titanium implant‐immunohistochemical study of RANKL, RANK, OPG: An experimental study in rats

Kim

Hwang

et al. 2007

Lasers Surg Med

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“…RANKL promotes the formation of osteoclast by bonding with cytokine, which influences bone remodeling. OPG inhibits the formation of osteoclast by inhibiting RANKL [9].…”

Section: Introductionmentioning

confidence: 99%

Effect of low‐level laser treatment after installation of dental titanium implant‐immunohistochemical study of RANKL, RANK, OPG: An experimental study in rats

Kim

Hwang

et al. 2007

Lasers Surg Med

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“…Increased RANKL/OPG ratio accompanies bone resorption involved in a variety of processes such as pathological osteolysis (Grimaud et al, 2003;Tay et al, 2004), periodontitis (Crotti et al, 2003), tooth eruption (Heinrich et al, 2005), and orthodontic tooth movement (Yamaguchi, 2009). The current data adds physiologic mandibular periosteal growth to that list.…”

Section: Discussionmentioning

confidence: 87%

Molecular Variations Related to the Regional Differences in Periosteal Growth at the Mandibular Ramus

Sun

Tee

2010

The Anatomical Record

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Periosteal growth at human mandibular ramus is characterized by bone apposition at the posterior border and resorption at the anterior border. Molecular control of this regional variation is unclear. This study examined the expression of several molecules involved in bone apposition/ resorption at these regions in vivo and in vitro. By using growing pigs as a model, the periosteal growth was assessed at the mandibular ramus by vital staining and histological observations. In parallel, periosteal tissues were harvested and pulverized for RNA and protein extraction. Periosteal cells were also isolated, expanded in osteogenic media, and subjected to a single dose of dynamic tensile strain (0, 5, or 10% magnitude at 0.5 Hz) to examine their responses to mechanical loading. Real-time RT-PCR and Western blot analyses were used to examine mRNA and protein expression from periosteal tissues and cultured cells. Histological observation confirmed an anterior-resorption/posterior-apposition pattern in the pig mandibular ramus. Both in vivo tissue and in vitro cells demonstrated greater mRNA expression of receptor activator of NF-jB ligand (RANKL)/osteoprotegerin (OPG) ratio and bone morphogenetic protein 2 (BMP2) at the anterior region, while OPG expression at the anterior region was lower than the posterior region. In response to the application of a single dose of dynamic tensile strain, cultured periosteal cells appeared to change the expression profile of osteogenic markers but not that of RANKL/OPG and BMP2. These findings suggest that the unique regional variation of periosteal activity at the mandibular ramus is regulated by a differential expression of RANKL/OPG ratio (likely through differential induction of OPG) and BMP2. Anat Rec, 294:79-87, 2011. V V C 2010 Wiley-Liss, Inc.Key words: periosteum; mandibular growth; RANKL/OPG; tissue engineering; cell loadingHuman mandibular growth is mainly through endochondral bone formation at the condyles and intramembranous bone formation at the periosteum (Enlow and Hans, 1996). While condylar growth provides the overall elongation and rotation of the mandible (Bjork and Skieller, 1983), periosteal activity causes extensive surface modeling/remodeling or reshaping of the mandible (Enlow and Hans, 1996). One striking feature of mandibular periosteal activity is at the ramus, where bone resorption takes place at the anterior (rostral) border whereas bone apposition at the posterior (caudal) border (Robinson and Sarnat, 1955;Seiton and Engel, 1969;Hans et al., 1995). At the cell level, osteogenic proliferation is concentrated at the appositional posterior ramal region, whereas osteoclasts are mainly present at the resorptive anterior region (Ochareon and Herring, 2007). However, the molecular mechanisms related to these differential periosteal activities are mostly unknown.

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“…Macrophage colony-stimulating factor (M-CSF), receptor activator of nuclear factor-kappaB ligand (RANKL), and osteoprotegerin (OPG), which regulate osteoclast differentiation and activation, have been identified in ameloblastomas and keratocystic odontogenic tumors (46,(166)(167)(168)(169)(170). These observations suggest that osteolytic cytokines have a role in local bone resorption during the progression of odontogenic tumors.…”

Section: Osteolytic Cytokinesmentioning

confidence: 99%

Molecular alterations in the development and progression of odontogenic tumors

Kumamoto

2010

Oral Med Pathol

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Odontogenic tumors are lesions derived from the elements of the tooth-forming apparatus and are found exclusively within the jawbones. This review presents a contemporary outline of our current understanding of the molecular and genetic alterations associated with the development and progression of odontogenic tumors, including apoptosis-related factors, telomerase, cell cycle regulators, growth factors, regulators of tooth development, hard tissue-related proteins, cell adhesion molecules, matrix-degrading proteinases, angiogenic factors, and osteolytic cytokines. It is hoped that better understanding of related molecular mechanisms will help to predict the course of odontogenic tumors and lead to the development of new therapeutic concepts for their management.

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Identification of RANKL in Osteolytic Lesions of the Facial Skeleton

Cited by 63 publications

References 28 publications

Effect of low‐level laser treatment after installation of dental titanium implant‐immunohistochemical study of RANKL, RANK, OPG: An experimental study in rats

Effect of low‐level laser treatment after installation of dental titanium implant‐immunohistochemical study of RANKL, RANK, OPG: An experimental study in rats

Molecular Variations Related to the Regional Differences in Periosteal Growth at the Mandibular Ramus

Molecular alterations in the development and progression of odontogenic tumors

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