Identification of Rapd Marker Linked to Resistance of Musa to Fusarium Oxysporum

Zambrano, Asia Y.; Martínez, Gustavo; Gutiérrez, Zulay; Manzanilla, Edward; Ruíz, Lucía Delgadillo; Díaz, Lucy M.; Contreras, A.; Márquez, Akira Pérez; Demey, Jhonny R.

doi:10.17660/actahortic.2007.738.69

Cited by 3 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…cubense (E.F. Smith) W.C. Snyder & H.N. Hansen (Foc) (Zambrano et al, 2007), est l'infection la plus destructrice du bananier (Ploetz et Pegg, 2000). Quatre races ont été identifiées chez Foc par leur capacité à infecter un cultivar de bananier spécifique (Groenewald, 2006).…”

Section: Introductionunclassified

Diagnostic de la fusariose du bananier sur les cultivars grande naine (Musa AAA) et corne 1 (Musa AAB) à la périphérie du District d’Abidjan (Côte d’Ivoire)

Kra¹,

Diallo²,

Kobenan³

et al. 2012

Int. J. Bio. Chem. Sci

View full text Add to dashboard Cite

En 2006, des symptômes ressemblant à la fusariose ont été observés sur des bananiers Grande Naine et Corne 1 en Côte d'Ivoire. Des échantillons de 5 mg de sol de la rhizosphère, trois bouts de racine et trois sections de faisceaux vasculaires de stipe des bananiers infectés ont été ensemencés séparément sur le milieu PDA pour l'isolement des champignons associés aux symptômes. Ces échantillons ont développé les 42 isolats de Fusarium oyxsporum identifiés à l'aide des clefs d'identification. En vue de connaître l'agent pathogène responsable de la maladie, un test de pathogénicité a été effectué. Neuf différents isolats de F. oxysporum isolés ont été inoculés à des vitroplants de Grande Naine et des vivoplants de Corne 1 sains en conditions contrôlées. Huit sur les neuf isolats testés ont reproduit les mêmes symptômes de la maladie observés au champ sur les deux cultivars de bananiers. Pour vérifier le Postulat de Koch, des explants de racines et de stipes issus des plants ayant développé les symptômes de la maladie ont été ensemencés sur le milieu PDA. Chaque explant d'organe a développé l'isolat initialement inoculé aux plants de bananier infectés.

show abstract

Section: Introductionunclassified

Diagnostic de la fusariose du bananier sur les cultivars grande naine (Musa AAA) et corne 1 (Musa AAB) à la périphérie du District d’Abidjan (Côte d’Ivoire)

Kra¹,

Diallo²,

Kobenan³

et al. 2012

Int. J. Bio. Chem. Sci

View full text Add to dashboard Cite

show abstract

The development and utilization of two SCAR markers linked to the resistance of banana (Musa spp. AAA) to Fusarium oxysporum f. sp. cubense race 4

Qv,

Feng,

Chen

et al. 2024

Euphytica

View full text Add to dashboard Cite

Banana (Musa spp.) production worldwide is seriously threatened by Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc). The best way to control this disease is to grow resistant cultivars. However, it requires large-scale field evaluations and labor- and time-consuming to obtain disease-resistant germplasm. Development of early, reliable, and reproducible selection strategies are considered as the efficient approach which could speed up the selection procedure. In this study, two pairs of sequence-related amplified polymorphism (SRAP) primers related to banana Foc resistance/susceptibility were screened from 100 pairs of random primers. Correspondingly, two pairs of sequence characterized amplified region (SCAR) markers (namely SC4-F/SC4-R and SC14-F/SC14-R, respectively) were successfully generated from these two SRAP markers using 30 cultivars either resistant or susceptible to Foc. Both SCAR markers were located in mitochondrion genome and showed discriminatory power of 96.67% and 100%, respectively. Mitochondrial proteins possibly play a very important role in banana resistance to Foc. In additional, these two SCAR markers were employed simultaneously to screen potential resistant germplasm from 53 accessions with unknown resistance to Foc, and the results revealed a consistency of 83.0% with each other, further indicating their high reliability and reproducibility. These results suggest that both SCAR markers could be used in molecular marker-assisted selection for banana germplasm resistant to Fusarium.

show abstract

Development of SCAR Markers Related to Banana Susceptibility to Fusarium oxysporum and Their Combination Use in Screening Potential Resistant Germplasm

Qv,

Feng,

Chen

et al. 2023

Preprint

View full text Add to dashboard Cite

Banana (Musa spp.) production worldwide is seriously threatened by Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc). The best way to control this disease is to grow resistant cultivars. However, it requires large-scale field evaluations and labor- and time-consuming to obtain disease-resistant germplasm. Development of early, reliable, and reproducible selection strategies are considered as the efficient approach which could speed up the selection procedure. In this study, two pairs of sequence-related amplified polymorphism (SRAP) primers related to banana Foc resistance/susceptibility were screened from 100 pairs of random primers. Correspondingly, two pairs of sequence characterized amplified region (SCAR) markers (namely SC4-F/SC4R and SC14-F/SC14R, respectively) were successfully generated from these two SRAP markers using 30 cultivars either resistant or susceptible to Foc. Both SCAR markers were located in mitochondrion genome and showed discriminatory power of 96.67% and 100%, respectively. In additional, these two SCAR markers were employed simultaneously to screen potential resistant germplasm from 53 accessions with unknown resistance to Foc, and the results revealed a consistency of 83.0% with each other, further indicating their high reliability and reproducibility. These results suggest that both SCAR markers could be used in molecular marker-assisted selection for banana germplasm resistant to Fusarium.

show abstract

Identification of Rapd Marker Linked to Resistance of Musa to Fusarium Oxysporum

Cited by 3 publications

References 0 publications

Diagnostic de la fusariose du bananier sur les cultivars grande naine (<em>Musa</em> AAA) et corne 1 (<em>Musa</em> AAB) à la périphérie du District d’Abidjan (Côte d’Ivoire)

Diagnostic de la fusariose du bananier sur les cultivars grande naine (<em>Musa</em> AAA) et corne 1 (<em>Musa</em> AAB) à la périphérie du District d’Abidjan (Côte d’Ivoire)

The development and utilization of two SCAR markers linked to the resistance of banana (Musa spp. AAA) to Fusarium oxysporum f. sp. cubense race 4

Development of SCAR Markers Related to Banana Susceptibility to Fusarium oxysporum and Their Combination Use in Screening Potential Resistant Germplasm

Contact Info

Product

Resources

About