Identification of reference genes for studies of quantitative gene expression in male and female quail tissues

Macário, Maíse dos Santos; Nascimento, Carlos Souza do; Sousa, Fabiana Cristina Belchior de; Oliveira, I. R. S.; Vesco, Ana Paula Del; Barbosa, Leandro Teixeira; Sousa, Katiene Régia Silva

doi:10.1080/10495398.2022.2092744

Cited by 1 publication

(1 citation statement)

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“…Previously, in magnum tissue of hens housed in CF systems, the most stable gene was HMBS, similar to our results in the spleen of hens of the CF (geNorm and NormFinder) and comprehensive data (CC-CF; BestKeeper) group ( Rodríguez-Hernández et al , 2021 ). On the other hand, the HMBS gene was reported as the most stable gene in the spleen of quail ( Macario et al , 2022 ) and codifying for HMBS protein, a vital enzyme in the heme biosynthetic pathway ( Wang et al , 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of reference genes for expression studies in the liver, and spleen of laying hens housed in cage and cage-free systems

nchez¹,

Villegas²,

Iang³

et al. 2023

Open Vet J

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Background: The liver and spleen play a pivotal role in metabolism and immune response. During stress, neuroendocrine response induces changes in gene expression and its assessment demands the validation of the stability of the reference genes to perform relative gene expression experiments. Aim: The objective of this study was to determine the expression stability of four reference genes (GAPDH, ACTB, RNA18S, and HMBS) in the liver and spleen tissues from laying hens housed in conventional cage (CC) and cage-free (CF) egg production systems. Methods: Liver and spleen from Hy-Line Brown hens housed in CC and CF egg production systems were used. mRNA transcript levels were determined by qPCR and the gene expression stability was evaluated using geNorm, BestKeeper and NormFinder algorithms. Results: the most stable gene from liver tissue was ACTB in CC, CF and CC-CF group (overall data). In the spleen, the most stables genes were GAPDH (CC), HMBS (CF), and ACTB (CC-CF). Conclusion: the ACTB gene was the most stable gene in the liver, and GAPDH and HMBS genes were stable in spleen tissues that could be used for the normalization in qPCR experiments performed in liver and spleen tissues of laying hens housed CC and CF production systems.

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Section: Discussionmentioning

confidence: 99%