Identification of resistance gene analogs involved in Phytophthora capsici recognition in black pepper (Piper nigrum L.)

Suraby, Erinjery Jose; Prasath, D.; Babu, K. Nirmal; Anandaraj, M.

doi:10.1007/s42161-020-00586-3

Cited by 14 publications

(3 citation statements)

References 34 publications

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“…Upon recognition of the effector, conformational changes allow the exchange of ADP to ATP, resulting in an open structure and the activation of downstream defense (Takken and Goverse, 2012). Phosphorylation of ADP has been reported as a key process in pathogen recognition (Suraby et al, 2020). The involvement of the NBS region of the I2 gene in binding to ATP at the ATP-binding sites of P-loop, kinase 2, and kinase 3, and the subsequent hydrolysis of ATP, confirmed the relatedness of NBS to the ATPase super family (Tameling et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Resistance screening and in silico characterization of cloned novel RGA from multi-race resistant lentil germplasm against Fusarium wilt (Fusarium oxysporum f. sp. lentis)

et al. 2023

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Fusarium wilt caused by Fusarium oxysporum f. sp. lentis (Fol) is the most devastating disease of lentil present worldwide. Identification of multi-race fusarium wilt resistance genes and their incorporation into existing cultivars will help to reduce yield losses. In the present study, 100 lentil germplasms belonging to seven lentil species were screened against seven prevalent races of Fol, and accessions IC201561 (Lens culinaris subsp. culinaris), EC714243 (L. c. subsp. odemensis), and EC718238 (L. nigricans) were identified as resistant. The typical R gene codes for the nucleotide-binding site and leucine-rich repeats (NBS-LRR) at the C terminal are linked to either the Toll/interleukin 1-like receptor (TIR) or coiled coil (CC) at the N terminal. In the present study, degenerate primers, designed from the NBS region amplifying the P-loop to the GLPLA motif, isolated forty-five resistance gene analogues (RGAs) from identified resistant accessions. The sequence alignment identified both classes of RGAs, TIR and non-TIR, based on the presence of aspartate (D) and tryptophan (W) at the end of the kinase motif, respectively. The phylogenetic analysis grouped the RGAs into six classes, from LRGA1 to LRGA6, which determined the diversity of the RGAs present in the host. Grouping of the RGAs identified from Lens nigricans, LnRGA 2, 9, 13 with I2 revealed the structural similarity with the fusarium resistance gene. The similarity index ranged from 27.85% to 86.98% among the RGAs and from 26.83% to 49.41% among the known R genes, I2, Gpa2, M, and L6. The active binding sites present along the conserved motifs grouped the RGAs into 13 groups. ADP/ATP, being the potential ligand, determines the ATP binding and ATP hydrolysis activity of the RGAs. The isolated RGAs can be used to develop markers linked to the functional R gene. Furthermore, expression analysis and full-length gene isolation pave the path to identifying the molecular mechanism involved in resistance.

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Section: Discussionmentioning

confidence: 99%

Resistance screening and in silico characterization of cloned novel RGA from multi-race resistant lentil germplasm against Fusarium wilt (Fusarium oxysporum f. sp. lentis)

et al. 2023

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“…Plants have evolved a complex innate immune system that allows them to recognize specific pathogens via a specialized group of gene products known as resistance (R) proteins (Cheng et al., 2016). RGAs are involved in plant disease resistance (Reddy et al., 2015; Suraby et al., 2020). In this study, the expression of miR168‐3p and its target gene RGA had opposite trends in the leaves after infection with BLS, which confirmed that RGA may be regulated by hvu‐miR168‐3p during BLS‐ resistance in Tibetan hulless barley.…”

Section: Discussionmentioning

confidence: 99%

Identification microRNAs and target genes in Tibetan hulless barley to BLS infection

et al. 2021

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Tibetan hulless barley (Hordeum vulgare L. variety nudum Hook. f.) is widely grown on the Qinghai-Tibet Plateau, where it has served as a staple food for Tibetan people since the 5th century CE. Barley leaf stripe (BLS) is one of the most severe fungal diseases affecting the yield and quality of Tibetan hulless barley. Here, we compared the miRNA profiles before and after BLS in a BLS-sensitive ('Z1141') and tolerant ('Kunlun14') Tibetan hulless barley for the first time. A total of 36 conserved and 56 novel miRNAs were identified, of which 24 differentially expressed miRNAs were found in Z1141 and Kunlun14, and an additional 546 putative target genes were predicted. Transcriptome sequencing analysis showed that among these genes, 131 were differentially expressed in normal and infected leaves of Kunlun14 and Z1141.Gene ontology (GO), pathway, and Blast analyses indicated 10 candidate target genes including seven functional genes and three transcription factors were involved in barley disease resistance. These functional genes were two members of the CYP450 genes, a RGA gene, a LIN gene, a SAM gene, a PSD gene, and a NDB gene; the three transcription factor genes were all from the WRKY family. These 10 candidate target genes were regulated by eight miRNAs (hvu-miR168-3p, hvu-miR171-5p, hvu-miR159b, hvu-miR156a, hvu-novel-91, hvu-novel-46, hvu-novel-52, and hvu-novel-11). According to quantitative real-time polymerase chain reaction (PCR) results, the 10 targets were negatively correlated with their corresponding miRNAs after infection with BLS. Thus, these miRNAs and their target genes can be used to improve BLS resistance in Tibetan hulless barley though breeding programs or genetic engineering.

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“…Plants evolved a complex innate immune system that recognized speci c pathogens via a speci c group of gene products called resistance (R) proteins [50]. RGAs were involved in plant disease resistance [51,52]. Thus, RGA appears to be crucial to the response of plant disease resistance.…”

Section: Discussionmentioning

confidence: 99%

Identification and Profiling of microRNAs and Their Target Genes in Tibetan Hulless Barley in Response to Barley Leaf Stripe (BLS) Infection

Yao¹,

Wang²,

Yao³

et al. 2020

Preprint

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Background Tibetan hulless barley is widely grown on the Qinghai-Tibet Plateau, where it has served as a staple food for Tibetan people since the 5th century CE. Barley leaf stripe (BLS) is one of the most severe fungal diseases affecting the yield and quality of Tibetan hulless barley. Results Here, we compared the miRNA profiles before and after BLS in two Tibetan hulless barley genotypes: Z1141, a BLS-sensitive wild variety, and Kunlun14, a BLS-tolerant hybrid variety. A total of 36 conserved and 56 novel miRNAs were identified. Of these, 10 conserved and 10 novel miRNAs exhibited significantly changed expression between the normal and infected leaves of Kunlun14, respectively, while 3 conserved and 5 novel miRNAs exhibited significantly changed expression between the normal and infected leaves of Z1141, respectively. A total of 24 miRNAs were found in Z1141 and Kunlun14, and a further 546 putative target genes were predicted. Transcriptome sequencing analysis showed that among the 546 candidate genes, 131 had significant differences in expression between the normal and infected leaves of Kunlun14 and Z1141. Gene ontology, pathway, and Blast analyses indicated 10 candidate target genes that were involved in the barley disease resistance. These 10 candidate target genes may be regulated by 7 miRNAs. According to quantitative real-time PCR results, the 10 targets were negatively correlated with their corresponding miRNAs after infection with BLS. Conclusions The miRNAs and their target genes expressed in Tibetan hulless barley were identified and found to be associated with BLS resistance. Thus, these miRNAs and their target genes may be exploited via breeding programs or genetic engineering to improve BLS resistance in Tibetan hulless barley.

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Identification of resistance gene analogs involved in Phytophthora capsici recognition in black pepper (Piper nigrum L.)

Cited by 14 publications

References 34 publications

Resistance screening and in silico characterization of cloned novel RGA from multi-race resistant lentil germplasm against Fusarium wilt (Fusarium oxysporum f. sp. lentis)

Resistance screening and in silico characterization of cloned novel RGA from multi-race resistant lentil germplasm against Fusarium wilt (Fusarium oxysporum f. sp. lentis)

Identification microRNAs and target genes in Tibetan hulless barley to BLS infection

Identification and Profiling of microRNAs and Their Target Genes in Tibetan Hulless Barley in Response to Barley Leaf Stripe (BLS) Infection

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