Identification of resistance loci toward <i>Phytophthora sojae</i> in South Korean soybean plant introductions 407974B and 424487B

Bolanos-Carriel, Carlos; Batnini, Amine; McHale, Leah K.; Dorrance, Anne E.

doi:10.1002/csc2.20596

Cited by 4 publications

(2 citation statements)

References 82 publications

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“…Rps loci for multiple generations of OH-Windfall and one generation of OH-MIA were mapped past the known end of the long arm of chromosome 18 in both the PI 407985 and PI 408097 populations. Resistance toward isolates OH4 and OH25 also mapped to the end of chromosome 18 in the Williams × PI 407974B RIL population by Bolaños-Carriel et al. (2022) .…”

Section: Discussionmentioning

confidence: 99%

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Comparison of Rps loci toward isolates, singly and combined inocula, of Phytophthora sojae in soybean PI 407985, PI 408029, PI 408097, and PI424477

Clevinger,

Biyashev,

Schmidt

et al. 2024

Front. Plant Sci.

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For soybean, novel single dominant Resistance to Phytophthora sojae (Rps) genes are sought to manage Phytophthora root and stem rot. In this study, resistance to P. sojae was mapped individually in four recombinant inbred line (RIL) populations derived from crosses of the susceptible cultivar Williams with PI 407985, PI 408029, PI 408097, and PI424477 previously identified as putative novel sources of disease resistance. Each population was screened for resistance with five to seven isolates of P. sojae separately over multiple F7–F10 generations. Additionally, three of the populations were screened with inoculum from the combination of three P. sojae isolates (PPR), which comprised virulence to 14 Rps genes. Over 2,300 single-nucleotide polymorphism markers were used to construct genetic maps in each population to identify chromosomal regions associated with resistance to P. sojae. Resistance segregated as one or two genes to the individual isolates and one gene toward PPR in each population and mapped to chromosomes 3, 13, or 18 in one or more of the four RIL populations. Resistance to five isolates mapped to the same chromosome 3 region are as follows: OH7 (PI 424477 and PI408029), OH12168, OH7/8, PPR (PI 407985), and 1.S.1.1 (PI408029). The resistance regions on chromosome 13 also overlapped for OH1, OH25, OH-MIA (PI424477), PPR (PI 424477, PI 407985, and PI 408097), PPR and OH0217 (PI 408097), and OH4 (PI 408029), but were distinct for each population suggesting multiple genes confer resistance. Two regions were identified on chromosome 18 but all appear to map to known loci; notably, resistance to the combined inoculum (PPR) did not map at this locus. However, there are putative new alleles in three of four populations, three on chromosome 3 and two on chromosome 13 based on mapping location but also known virulence in the isolate used. This characterization of all the Rps genes segregating in these populations to these isolates will be informative for breeding, but the combined inoculum was able to map a novel loci. Furthermore, within each of these P. sojae isolates, there was virulence to more than the described Rps genes, and the effectiveness of the novel genes requires testing in larger populations.

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Section: Discussionmentioning

confidence: 99%

“…Waseshiroge and Rps of cv. Daewon, and Rps identified in PI 407974B ( Bolaños-Carriel et al., 2022 ; Lin et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Rps loci toward isolates, singly and combined inocula, of Phytophthora sojae in soybean PI 407985, PI 408029, PI 408097, and PI424477

Clevinger,

Biyashev,

Schmidt

et al. 2024

Front. Plant Sci.

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Erratum to: Identification of resistance loci toward Phytophthora sojae in South Korean soybean plant introductions 407974B and 424487B

Bolanos-Carriel¹,

Batnini²,

McHale³

et al. 2022

Crop Science

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Resistance gene enrichment sequencing for NLR genes for Phytophthora sojae in selected soybean plant introductions and differentials with putative novel and known Rps genes

Hodge,

Batnini,

Bolaños‐Carriel

et al. 2024

Crop Science

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Numerous sources of putative novel resistance genes toward Phytophthora sojae (Rps genes) have been identified and loci mapped in soybean (Glycine max L. Merr.) but cloning has remained elusive. We utilized resistance gene enrichment sequencing (RenSeq) to identify the putative resistance genes in 20 plant introductions (PIs) and differentials of the cultivar Williams with rps, Rps1c, Rps3a, and Rps8. The DNA from these genotypes was enriched and sequenced using more than 25,000 80 nt baits designed to nucleotide‐binding leucine‐rich repeat (NLR) encoding sequences. Overall, there were greater numbers of variants in the NLR‐encoding genes in Rps loci on chromosomes (Chrs) 3, 7, 13, and 18 for the 20 PIs as compared to the Williams differentials for rps, Rps1c, Rps1k, Rps3a, and Rps8. Genes encoding Rps1c, Rps3a, and Rps8 were proposed based on sequence differences among the differentials. Among the 20 PIs, there may be additional alleles on Chrs 3, 13, and 18, and PI399079 may have two new alleles at Chrs 3 and 7 loci. A unique NLR on Chr 8 was identified in PI200553. New alleles were also identified on Chrs 3 and 18 when the PI and resistant bulks were compared to susceptible recombinant inbred lines. This study demonstrates the utility of RenSeq as an efficient method to identify and predict specific novel NLR genes in landrace soybean germplasm, which confer resistance to P. sojae and obtain gene‐specific markers to facilitate their introgression into modern cultivars.

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Identification of resistance loci toward Phytophthora sojae in South Korean soybean plant introductions 407974B and 424487B

Cited by 4 publications

References 82 publications

Comparison of Rps loci toward isolates, singly and combined inocula, of Phytophthora sojae in soybean PI 407985, PI 408029, PI 408097, and PI424477

Comparison of Rps loci toward isolates, singly and combined inocula, of Phytophthora sojae in soybean PI 407985, PI 408029, PI 408097, and PI424477

Erratum to: Identification of resistance loci toward Phytophthora sojae in South Korean soybean plant introductions 407974B and 424487B

Resistance gene enrichment sequencing for NLR genes for Phytophthora sojae in selected soybean plant introductions and differentials with putative novel and known Rps genes

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