Identification of Rice Blast Resistance Genes in South Central Coast of Vietnam Using Monogenic Lines under Field Condition and Pathogenicity Assays

Abstract

Abstract:In the present study, the authors studied the interaction between 26 monogenic differentials carrying 26 blast resistance genes with 15 isolates of Pyricularia oryzae under field conditions and pathogenicity assays. The result in field conditions showed that area under disease progress curves (AUDPC) of monogenic lines was found to range from 0 to 142.3. Lijiangxintuanheigu (LTH) was susceptible in all of regions. The monogenic lines carrying Pik, Piz, Pi1, Pi7(t), Pi4(t), Pish, Pi9(t) and Pita were highly resistant to blast in all of regions. In plastic house net conditions, the percentage of virulent reactions of monogenic lines to 15 isolates was found to range from 23.1% to 84.6%. LTH was susceptible to all 15 isolates. All 26 monogenic lines were resistant to at least 3 isolates of P. oryzae, and the frequency of resistant reactions of the monogenic lines carrying Pik, Piz, Pi4(t), Pish, Pi1, Pi7(t), Pi9, Pik-m and Pita were 80.0%, 93.3%, 93.3%, 80.0%, 73.3%, 86.7%, 73.3%, 80.0%, 80.0%, 80.0% and 80.0%, respectively. These findings suggest that Pik, Piz, Pi4(t), Pish, Pi1, Pi7(t), Pi9, Pik-m and Pita may be important R genes for preventing blast disease in South Central Vietnam. Based on these data, a useful strategy for managing rice blast disease by stacking pyramiding blast R genes against pathogenic P. oryzae isolates at hotspot in South Central Coast of Vietnam was proposed.

New Climate Resilience Rice Varieties for Coping with Climate Change HomThadokKham 17 (HTDK 17)

“…These varieties are also tolerant to flooding, lodging resistant, medium-height, resistant to shattering, mature at medium-season, widely adaptable, suitable for harvesting machinery, and have soft eating quality and aroma. Reports of resistance to the blast disease isolate have been documented in Thailand and Laos [5,15,22], the Philippines [5], Cambodia [7], Vietnam [18]. While over 100 genes are known to confer resistance to blast disease, only 24 have been successfully cloned [34].…”

A unique genetic variation with respect to blast (<i>Pyricularia oryzae</i> Cavara) resistance in rice (<i>Oryza sativa</i> L.) varieties in Vietnam

“…Since improved rice cultivars were introduced in Vietnam after green revolution with the release of improved rice cultivars carrying the semi-dwarf gene, rice yields have significantly increased, allowing both national food security and export ( Duong and Thanh 2019 ). However, those cultivars were introduced from nearby countries such as Thailand and China and from the International Rice Research Institute (IRRI), and their genetic backgrounds of rice cultivars introduced in Vietnam have been therefore poorly understood ( Nguyen et al 2015 ). Thus, the necessity of clarification of the genetic variation in rice germplasm has remained in Vietnam.…”

“…The MLs and LTH NILs have been used widely as international DVs owing to their high ability to differentiate races of blast fungus from tropical to temperate areas: Cambodia ( Fukuta et al 2014 ), West Africa ( Odjo et al 2014 ), south central coast of Vietnam ( Nguyen et al 2015 ), Yunnan, China ( Li et al 2016 ), Japan ( Kawasaki-Tanaka et al 2016 ), Kenya ( Fukuta et al 2019a ), Northern and Central Vietnam ( Nguyen et al 2020 ), Mekong River Delta, Vietnam ( Fukuta et al 2020 ), Laos ( Xangsayasane et al 2020 ), and Indonesia ( Kadeawi et al 2021 ). Specifically, the differential systems consist of DVs and standard differential blast isolates (SDBIs) that were developed based on pathological analyses from West Africa ( Odjo et al 2016 ), Bangladesh ( Khan et al 2016 ), Vietnam ( Nguyen et al 2020 ), Laos ( Xangsayasane et al 2020 ), and Indonesia ( Kadeawi et al 2021 ), and have been used in studies on pathogenicity of blast fungus and resistance genes in rice cultivars.…”

Investigating Rice Blast Resistance Gene Distribution among Landrace Rice Varieties in Lower Northern Thailand for Improving Rice Cultivars

“…More recently, rice blast resistance genes were characterized in landrace rice from various locations such as Bangladesh, China, Japan, Malaysia, Tailand, and Vietnam. From these, more than 30 R genes were identifed in Asian landrace rice, i.e., Pi-36, Pi9, Pib, Pigm(t), Pik-p, Pik-h, Pi-ta, Piz [7,[20][21][22][23]. Of these 30 R genes, the Pi9, Pib, and Pi-ta were commonly found in resistance rice cultivars grown in Malaysia [20] and Tailand [7].…”