Identification of RNA helicases in Medicago truncatula and their expression patterns under abiotic stress

Cheng, Jie; Zhou, Songsong; Yang, Kun; Yu, Haifeng; Chen, Rongrong; Zeng, Liming; Li, Hua; Wang, Yihua; Song, Jianbo

doi:10.1007/s12298-021-01087-y

Cited by 2 publications

(1 citation statement)

References 37 publications

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“…It seems therefore likely that, in addition of CreRH22, several other plastid-targeted DEAD-box RNA helicases may participate, via the nuclear control of the plastid protein synthesis, to the coordination between nuclear and plastid compartments, which is critical for a correct assemblage of chloroplast complexes, particularly in changing light conditions. Recently, many DEAD-box RNA helicases have been identified and partially characterized in crop plant genomes, including rice (Lu et al, 2020), wheat (Ru et al, 2021), Medicago truncatula (Cheng et al, 2021) and Brassica rapa (Nawaz et al, 2021), and plastid-targeted DEAD-box RNA helicases have been proposed to play a role in the response to abiotic stress such a drought, salinity or cold (Nawaz and Kang, 2017). As light is as a key parameter that potentiates the effects of abiotic stress on photosynthesis (Schwenkert, in press), plastid-targeted DEAD-box RNA helicases may represent promising targets towards improving the productivity of crops in stress conditions.…”

Section: Discussionmentioning

confidence: 99%

A plastidial DEAD box RNA helicase plays a critical role in high light acclimation by modulating ribosome biogenesis inChlamydomonas reinhardtii

Djouani-Tahri

Nellaepalli

Auroy

et al. 2022

Preprint

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Photosynthetic organisms have developed sophisticated strategies to fine-tune light energy conversion to meet the metabolic demand, thereby optimizing growth in fluctuating light environments. Although mechanisms such as energy dissipation, photosynthetic control, or the photosystem II (PSII) damage and repair have been widely studied, little is known about the regulation of protein synthesis capacity during light acclimation. By screening a Chlamydomonas reinhardtii insertional mutant library using chlorophyll fluorescence imaging, we isolated a high chlorophyll fluorescence mutant (hf0) defected in a gene encoding a putative plastid targeted DEAD-box RNA helicase called CreRH22. CreRH22 is rapidly induced upon illumination and belongs to the GreenCut, a set of proteins specific to photosynthetic organisms. While photosynthesis is slightly affected in the mutant under low light (LL), exposure to high light (HL) induces a marked decrease in both PSII and PSI, and a strong alteration of the light-induced gene expression pattern. These effects are explained by the inability of hf0 to increase plastid ribosome amounts under HL. We conclude that CreRH22, by promoting ribosomal RNA precursor maturation in a light-dependent manner, enables the assembly of extra-ribosomes required to synthesize photosystem subunits at a higher rate, a critical step in the acclimation of algae to HL.

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Section: Discussionmentioning

confidence: 99%

A plastidial DEAD box RNA helicase plays a critical role in high light acclimation by modulating ribosome biogenesis inChlamydomonas reinhardtii

Djouani-Tahri

Nellaepalli

Auroy

et al. 2022

Preprint

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Functions and mechanisms of RNA helicases in plants

Zhu

et al. 2022

Journal of Experimental Botany

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RNA helicases (RHs) are a family of ubiquitous enzymes that alter RNA structures and remodel ribonucleoprotein complexes (RNPs) using the energy typically through hydrolysis of adenosine 5′-triphosphate (ATP). RHs are involved in various aspects of RNA processing and metabolism, exemplified by transcriptional regulation, pre-mRNA splicing, miRNA biogenesis, liquid-liquid phase separation, and rRNA biogenesis among other molecular processes. Through these mechanisms, RHs contribute to vegetative and reproductive growth, as well as abiotic and biotic stress responses throughout the life cycle in plants. In this review, we systematically characterized RH-featured domains and signature motifs in Arabidopsis. We also summarized the functions and mechanisms of RHs in various biological processes in plants with a focus on DEAD-box and DEAH-box RNA helicases, aiming to present the latest pictures of RHs in plant biology.

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Identification of RNA helicases in Medicago truncatula and their expression patterns under abiotic stress

Cited by 2 publications

References 37 publications

A plastidial DEAD box RNA helicase plays a critical role in high light acclimation by modulating ribosome biogenesis inChlamydomonas reinhardtii

A plastidial DEAD box RNA helicase plays a critical role in high light acclimation by modulating ribosome biogenesis inChlamydomonas reinhardtii

Functions and mechanisms of RNA helicases in plants

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