Identification of S.aureus and E.coli from Dairy Products Intended for Human Consumption

Kandil, Aya Atef; Elhadidy, Mohamed; El-Gamal, Ahmed; Abdou, A. M.

doi:10.17582/journal.aavs/2018/6.11.509.513

Cited by 10 publications

(14 citation statements)

References 10 publications

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“…To some extent nearly similar results for the count were indicated by Ewida (2009) and Salama et al (2015), with respect to the S. aureus incidence higher results were recorded by El-Kholy et al (1995) and Kandil et al (2018), but lower results were reported by Mousa (2017) and Abo El-Makarem and Amer (2018). While lower results for the count were recorded by Moawad et al (2002) S. aureus is frequently found in milk, however their presence in kareish cheese is usually due to primary contamination from raw milk, unhygienic handling of the cheese and ∕ or on extensive contamination by personnel possibly involved in cheese making and marketing; as humans are the common carriers.…”

Section: Figure (3)supporting

confidence: 70%

“…The highest frequency distribution (36%) was in a range of 10 6 -<10 7 . Nearly similar result for the incidence was recorded by Abou El-Makarem (2013), while lower results for count and incidence were indicated by Hafez (2010) and Kandil et al (2018).…”

Section: Figure (3)supporting

confidence: 68%

“…To some extent nearly similar result for the count was pointed by Nawar (2001), but for the incidence was recorded by Ahmed et al (2019). While higher result for incidence was reported by Kandil et al (2018) and lower result for the count was detected by Ewida (2009). It's noticed from the results in Table (2) and figures (2&3) that the count of S. aureus in the examined large scale ice-cream ranged from <10 2 to 1.1×10 7 with a mean value of 6.52×10 5 ±4.41×10 5 CFU/g and an incidence of 24%.…”

Section: Figure (3)mentioning

confidence: 75%

“…In addition to, lower result for the incidence was reported by El-Ansary (2015). From another side higher results for the incidence were detected by Zakary et al (2011) and Kandil et al (2018).…”

Section: Figure (3)mentioning

confidence: 80%

“…,De Oliveira et al (2011), Charaya et al (2014 andPourtaghi et al (2015). While higher results were reported byGitau et al (2010),Ayele et al (2017) at farm level, andKandil et al (2018). On the other side, lower results were demonstrated byAbdou et al (2016) andMatallah et al (2019).…”

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confidence: 80%

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Studies on Enterotoxigenic Staphylococcus Aureus in Milk and Some Dairy Products

Meshref

Hassan

Riad³

et al. 2019

Assiut Veterinary Medical Journal

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Staphylococcus aureus is one of the commonest aetiological agents of bacterial diseases. Food intoxication by S.aureus was listed as the third etiology of food intoxication all over the world. The current study was designed to record the prevalence of S. aureus in milk and some dairy products. A total of 200 samples consisting of (25 raw cow's milk, 25 raw buffalo's milk, 50 kareish cheese, 25 small scale ice-cream, 25 large scale ice-cream, 25 small scale yoghurt and 25 large scale yoghurt samples) were collected from dairy shops and street vendors in Beni-Suef Governorate, Egypt. The prevalence rate of S. aureus was 13 (52%), 16 (64%), 34 (68%), 20 (80%), 6 (24%), 22 (88%) and 9 (36%) with an average count of 1.62×10 8 ± 9.5×10 7 , 7.88×10 7 5.19×10 7 CFU/ml, 8.68×10 7 2.61×10 7 , 6.64×10 7 3.29×10 7 , 6.52×10 5 4.41×10 5 , 3.67× 10 61.68 × 10 6 and 5.27×10 5 3.45× 10 5 CFU/g in the concering samples, respectively. The molecular results confirmed that 30% of the examined S. aureus strains were enterotoxigenic by PCR technique as carried one or two SE-genes. High S. aureus counts in milk and milk products constitute a public health hazard to the consumers and emphasizes the need for improved hygienic standards.

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Section: Figure (3)supporting

confidence: 70%

Section: Figure (3)supporting

confidence: 68%

Section: Figure (3)mentioning

confidence: 75%

Section: Figure (3)mentioning

confidence: 80%

mentioning

confidence: 80%

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Studies on Enterotoxigenic Staphylococcus Aureus in Milk and Some Dairy Products

Meshref

Hassan

Riad³

et al. 2019

Assiut Veterinary Medical Journal

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Bacteriological quality of raw cow milk at a collection center in Iwo, Osun State, Nigeria

Elutade,

Imohiosen,

Akinola

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Dairy milk provides essential nutrients for humans, but microbial contamination can occur from the point of milking to storage. There has been sparsity of data on quality of raw cow milk (RCM) at collection points in Nigeria. This study investigated the bacteriological quality of RCM from a bulk-tank in Iwo, Osun State, South-West Nigeria. Raw milk samples were collected from the storage tank during a six-week period and evaluated for aerobic plate count (APC), aerobic spore-formers count, Enterobacteriaceae count, salmonella and shigella count and Staphylococcus count. One-way analysis of variance (ANOVA) and Duncan’s multiple range test were used to compare differences in means among the various bacterial counts assessed, using the Statistical Package for Social Sciences (SSPS). The mean aerobic plate count (8.2±1.71 log CFU/ml) and Enterobacteriaceae count (5.8±1.12 log CFU/ml) obtained were significantly different (P≤0.05) and were higher than the acceptable threshold limits of 5.0 log CFU/ml and 3.0 log CFU/ml, respectively. The bacteriological profile found in the milk samples included Enterobacter spp., Salmonella spp., Shigella spp., Staphylococcus spp., and Pseudomonas spp. Enterobacter spp. and Staphylococcus aureus had the highest and least occurrence of 30 % and 2.5 %, respectively. Good sanitary practices are recommended at every stage of the milking process prior to storage in a bulk-tank so as to ensure safe dairy derivative products.

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Prevalence and molecular characteristics of Staphylococcus aureus in raw milk and milk products in Ethiopia: a systematic review and meta-analysis

et al. 2022

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Background Staphylococcus aureus is an important opportunistic pathogen of raw milk and milk products, and the enterotoxins cause food poisoning. Milk and milk products are important reservoirs of enterotoxin-producing S. aureus. The aims of this systematic review were to estimate the pooled prevalence of S. aureus, including methicillin-resistant Staphylococcus aureus (MRSA), and to summarize their molecular characteristics, assess the potential sources of S. aureus contamination in bulk milk and analyse the antimicrobial resistance patterns of the isolates. Methods Following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, we searched publicly available scientific online databases and search engines: PubMed, Research for Life, African Journal Online (AJOL), and Google Scholar. In addition, the reference lists of the identified studies were manually checked for relevant literature. A random effects model using the DerSimonian method was used to compute pooled prevalence estimates, and the data were transformed using variance stabilizing Freeman-Tukey double arcsine transformation. Results A total of 38 studies were included in this systematic review. The pooled prevalence of S. aureus was highest in raw cow milk (30.7%), followed by camel milk (19.3%), goat milk (13.6%) and pasteurized milk (3.8%). The pooled prevalence of S. aureus in locally produced soft cheese (ayib) and traditional fermented milk (ergo) was 18.6% and 14.9%, respectively. The pooled prevalence of MRSA in milk and milk products was 0.73%. In this study, 58.9% of S. aureus isolates recovered from milk and milk products harbored at least one type of enterotoxin gene. Raw milk of the three species (cow, goat and camel) showed the highest S. aureus pooled prevalence rate at processing plants (50.3%), followed by milk collection centers (MCCs) (47.1%), selling points (34.5%), farm bulk milk (25.8%), milking buckets (24.8%) and udder milk (20.3%). Water for washing milking utensils (39.3%) was more contaminated than swab samples from farm workers’ nares (31.5%), milkers’ hands (25.9%), MCCs containers (23.8%), bulk tanks (20.4%), udders (15.6%), milking buckets (14.2%) and towels (10%). S. aureus isolates were highly resistant to penicillin G (92%), followed by ampicillin (82%) and amoxicillin (62.6%). The pooled multidrug resistance (MDR) was high (62.1%). Conclusion This systematic review revealed a high and increasing level of S. aureus contamination of raw milk from udder to MCCs or processing plants. Enterotoxin genes and MRSA were reported in milk, milk products and samples from farm workers. S. aureus showed resistance to different antimicrobial agents, with β-lactams showing the highest pooled antimicrobial resistance and the level of MDR was high. The results of this study indicated that the consumption of raw milk and milk products may predispose consumers to staphylococcal food poisoning. Application of good hygiene and handling practices across the dairy value chain starting from farm, udder health, milk cooling, heat treatment of milk before drinking and rational use of antibiotics in veterinary medicine can reduce the potential health risks from S. aureus and MRSA contamination of milk and milk products.

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Identification of S.aureus and E.coli from Dairy Products Intended for Human Consumption

Cited by 10 publications

References 10 publications

Studies on Enterotoxigenic Staphylococcus Aureus in Milk and Some Dairy Products

Studies on Enterotoxigenic Staphylococcus Aureus in Milk and Some Dairy Products

Bacteriological quality of raw cow milk at a collection center in Iwo, Osun State, Nigeria

Prevalence and molecular characteristics of Staphylococcus aureus in raw milk and milk products in Ethiopia: a systematic review and meta-analysis

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