Identification of Salivary Microorganisms and Metabolites Associated with Halitosis

Jo, Jae-Kwon; Seo, Seung-Ho; Park, Seong-Eun; Kim, Hyun Woo; Kim, Eunju; Na, Chang-Su; Cho, Kwang-Moon; Kwon, Sun-Jae; Moon, Young‐Jin; Son, Hong-Seok

doi:10.3390/metabo11060362

Cited by 19 publications

(12 citation statements)

References 55 publications

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“…Moreover, both genera correlated with increased VSC (here: H 2 S and CH 3 SH) values. This observation was in line with a Korean study where higher abundances of Prevotella and Alloprevotella in saliva correlated with amine metabolites, suggesting a causative mechanism via cadaverine and putrescine pathways [ 173 ].…”

Section: Prevotella Involvement In Oral Conditions Of Clinic...supporting

confidence: 91%

Prevotella species as oral residents and infectious agents with potential impact on systemic conditions

Könönen

Fteita

Gürsoy

et al. 2022

Journal of Oral Microbiology

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Oral Prevotella are known as anaerobic commensals on oral mucosae and in dental plaques from early life onwards, including pigmented P. melaninogenica, P. nigrescens, and P. pallens and non-pigmented Prevotella species. Many Prevotella species contribute to oral inflammatory processes, being frequent findings in dysbiotic biofilms of periodontal diseases ( P. intermedia, P. nigrescens ), cariotic lesions ( P. denticola, Alloprevotella (formerly Prevotella ) tannerae ), endodontic infections ( P. baroniae, P. oris, P. multisaccharivorax ), and other clinically relevant oral conditions. Over the years, several novel species have been recovered from the oral cavity without knowledge of their clinical relevance. Within this wide genus, virulence properties and other characteristics like biofilm formation seemingly vary in a species- and strain-dependent manner, as shown for the P. intermedia group organisms ( P. aurantiaca, P. intermedia, P. nigrescens , and P. pallens ). Oral Prevotella species are identified in various non-oral infections and chronic pathological conditions. Here, we have updated the knowledge of the genus Prevotella and the role of Prevotella species as residents and infectious agents of the oral cavity, as well as their detection in non-oral infections, but also gathered information on their potential link to cancers of the head and neck, and other systemic disorders.

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Section: Prevotella Involvement In Oral Conditions Of Clinic...supporting

confidence: 91%

Prevotella species as oral residents and infectious agents with potential impact on systemic conditions

Könönen

Fteita

Gürsoy

et al. 2022

Journal of Oral Microbiology

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“…Other studies have used isopropanol [ 208 ] or acetone [ 209 ], which requires evaporation and resuspension in an appropriate solvent before chromatographic analysis. When GC–MS is used, the dried extracts are derivatized using oximation followed by a silylation step [ 209 , 210 , 211 , 212 , 213 , 214 , 215 , 216 ]. Methods involving headspace [ 217 ] and solid-phase microextraction (SPME) for VOC analysis [ 218 ] are also found.…”

Section: Underexplored Specimens In Clinical Metabolomicsmentioning

confidence: 99%

Critical Factors in Sample Collection and Preparation for Clinical Metabolomics of Underexplored Biological Specimens

de Souza,

Pereira,

de Sá

et al. 2024

Metabolites

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This review article compiles critical pre-analytical factors for sample collection and extraction of eight uncommon or underexplored biological specimens (human breast milk, ocular fluids, sebum, seminal plasma, sweat, hair, saliva, and cerebrospinal fluid) under the perspective of clinical metabolomics. These samples are interesting for metabolomics studies as they reflect the status of living organisms and can be applied for diagnostic purposes and biomarker discovery. Pre-collection and collection procedures are critical, requiring protocols to be standardized to avoid contamination and bias. Such procedures must consider cleaning the collection area, sample stimulation, diet, and food and drug intake, among other factors that impact the lack of homogeneity of the sample group. Precipitation of proteins and removal of salts and cell debris are the most used sample preparation procedures. This review intends to provide a global view of the practical aspects that most impact results, serving as a starting point for the designing of metabolomic experiments.

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“…It is mostly GC–MS detection that is used for studying the VOCs present in saliva. This technique has already been applied in halitosis research, where a study recently identified two potential biomarkers of halitosis: 5-aminovaleric acid and N -acetylornithine [ 131 ]. Monedeiro et al have reported 164 VOCs in saliva, which they monitored by headspace solid-phase microextraction coupled to GC–MS (HS-SPME-GC–MS).…”

Section: Assessment Of Halitosismentioning

confidence: 99%

A Literature Review and Framework Proposal for Halitosis Assessment in Cigarette Smokers and Alternative Nicotine-Delivery Products Users

Zanetti

Semren

Battey

et al. 2021

Front. Oral. Health

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Halitosis is a health condition which counts cigarette smoking (CS) among its major risk factors. Cigarette smoke can cause an imbalance in the oral bacterial community, leading to several oral diseases and conditions, including intraoral halitosis. Although the best approach to decrease smoking-related health risks is quitting smoking, this is not feasible for many smokers. Switching to potentially reduced-risk products, like electronic vapor products (EVP) or heated tobacco products (HTP), may help improve the conditions associated with CS. To date, there have been few systematic studies on the effects of CS on halitosis and none have assessed the effects of EVP and HTP use. Self-assessment studies have shown large limitations owing to the lack of reliability in the participants' judgment. This has compelled the scientific community to develop a strategy for meaningful assessment of these new products in comparison with cigarettes. Here, we compiled a review of the existing literature on CS and halitosis and propose a 3-layer approach that combines the use of the most advanced breath analysis techniques and multi-omics analysis to define the interactions between oral bacterial species and their role in halitosis both in vitro and in vivo. Such an approach will allow us to compare the effects of different nicotine-delivery products on oral bacteria and quantify their impact on halitosis. Defining the impact of alternative nicotine-delivery products on intraoral halitosis and its associated bacteria will help the scientific community advance a step further toward understanding the safety of these products and their potentiall risks for consumers.

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Identification of Salivary Microorganisms and Metabolites Associated with Halitosis

Cited by 19 publications

References 55 publications

Prevotella species as oral residents and infectious agents with potential impact on systemic conditions

Prevotella species as oral residents and infectious agents with potential impact on systemic conditions

Critical Factors in Sample Collection and Preparation for Clinical Metabolomics of Underexplored Biological Specimens

A Literature Review and Framework Proposal for Halitosis Assessment in Cigarette Smokers and Alternative Nicotine-Delivery Products Users

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