Identification of salivary microRNA profiles in male mouse model of chronic sleep disorder

Yoshida, Yuta; Yajima, Yuhei; Fujikura, Yuri; Zhuang, Haotong; Higo-Yamamoto, Sayaka; Toyoda, Atsushi; Oishi, Katsutaka

doi:10.1080/10253890.2022.2156783

“…We have selected saliva as a biological matrix preferable to blood because in individuals without training, it can be collected easily and in a non-invasive way with a lower stress for the animals. Saliva was used in previous studies to evaluate stress in sheep [6,30,31] and, in some research, it has been used to evaluate miRNAs as biomarkers of stress in other species such as humans [32], pigs [33], and mice [34].…”

Section: Discussionmentioning

confidence: 99%

Adaptation Response in Sheep: Ewes in Different Cortisol Clusters Reveal Changes in the Expression of Salivary miRNAs

Manenti,

Viola,

Ala

et al. 2023

Animals

1

0

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Farm procedures have an impact on animal welfare by activating the hypothalamic-pituitary-adrenal axis that induces a wide array of physiological responses. This adaptive system guarantees that the animal copes with environmental variations and it induces metabolic and molecular changes that can be quantified. MicroRNAs (miRNAs) play a key role in the regulation of homeostasis and emerging evidence has identified circulating miRNAs as promising biomarkers of stress-related disorders in animals. Based on a clustering analysis of salivary cortisol trends and levels, 20 ewes were classified into two different clusters. The introduction of a ram in the flock was identified as a common farm practice and reference time point to collect saliva samples. Sixteen miRNAs related to the adaptation response were selected. Among them, miR-16b, miR-21, miR-24, miR-26a, miR-27a, miR-99a, and miR-223 were amplified in saliva samples. Cluster 1 was characterized by a lower expression of miR-16b and miR-21 compared with Cluster 2 (p < 0.05). This study identified for the first time several miRNAs expressed in sheep saliva, pointing out significant differences in the expression patterns between the cortisol clusters. In addition, the trend analyses of these miRNAs resulted in clusters (p = 0.017), suggesting the possible cooperation of miR-16b and -21 in the integrated stress responses, as already demonstrated in other species as well. Other research to define the role of these miRNAs is needed, but the evaluation of the salivary miRNAs could support the selection of ewes for different profiles of response to sources of stressors common in the farm scenario.

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“…10 These molecules, such as miRNAs, can be used as diagnostic biomarkers of tumors, autism, Parkinson disease and sleep disorder. 11 Only a few studies have investigated the irisin level in saliva. [12][13][14][15][16][17] Findings have shown that this myokine is present in a different concentration over blood.…”

mentioning

confidence: 99%

Salivary and serum irisin in healthy adults before and after exercise

Missaglia

¹

,

Tommasini²,

Vago³

et al. 2023

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Irisin is an exercise-induced cytokine mainly secreted by myocytes. Circulating level of irisin can increase in response to acute exercise, promoting pleiotropic effects on health. Generally, irisin is evaluated in blood, however, its collection is invasive. Saliva sample would not have any risk associated with blood collection and would represent a less invasive method for irisin detection. Until now, there are only a few studies that have analyzed irisin levels in saliva. In the present research, five healthy male adults performed an incremental exercise until exhaustion on cycle ergometer. Serum and saliva samples were collected before exercise and 15min, 24h and 48h after reaching the exhaustion. Irisin was detected by ELISA assay. Serum and salivary irisin levels increased from baseline to 24h post exercise and reverted to basal levels after 48h of rest. A significant rise of both serum and salivary irisin level at 24h (p≤0.05) compared to baseline levels was found. Furthermore, a significant correlation between irisin percentage change in serum and saliva from baseline to 24h post exercise was detected (r=0.92, p<0.05). Despite the relatively limited sample, this research suggests that collecting saliva samples might represent a valid and less invasive method to detect irisin level changes induced by exercise.

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Salivary and serum irisin in healthy adults before and after exercise

Missaglia

¹

,

Tommasini²,

Vago³

et al. 2023

Eur J Transl Myol

0

View full text Add to dashboard Cite

Irisin is an exercise-induced cytokine mainly secreted by myocytes. Circulating level of irisin can increase in response to acute exercise, promoting pleiotropic effects on health. Generally, irisin is evaluated in blood, however, its collection is invasive. Saliva sample would not have any risk associated with blood collection and would represent a less invasive method for irisin detection. Until now, there are only a few studies that have analyzed irisin levels in saliva. In the present research, five healthy male adults performed an incremental exercise until exhaustion on cycle ergometer. Serum and saliva samples were collected before exercise and 15min, 24h and 48h after reaching the exhaustion. Irisin was detected by ELISA assay. Serum and salivary irisin levels increased from baseline to 24h post exercise and reverted to basal levels after 48h of rest. A significant rise of both serum and salivary irisin level at 24h (p≤0.05) compared to baseline levels was found. Furthermore, a significant correlation between irisin percentage change in serum and saliva from baseline to 24h post exercise was detected (r=0.92, p<0.05). Despite the relatively limited sample, this research suggests that collecting saliva samples might represent a valid and less invasive method to detect irisin level changes induced by exercise.

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Identification of salivary microRNA profiles in male mouse model of chronic sleep disorder

Cited by 3 publications

References 56 publications

Adaptation Response in Sheep: Ewes in Different Cortisol Clusters Reveal Changes in the Expression of Salivary miRNAs

Adaptation Response in Sheep: Ewes in Different Cortisol Clusters Reveal Changes in the Expression of Salivary miRNAs

Salivary and serum irisin in healthy adults before and after exercise

Salivary and serum irisin in healthy adults before and after exercise

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