Identification of salivary protein biomarkers for orthodontically induced inflammatory root resorption

Kaczor‐Urbanowicz, Karolina Elżbieta; Deutsch, Omer; Zaks, Batia; Krief, Guy; Chaushu, Stella; Palmon, Aaron

doi:10.1002/prca.201600119

Cited by 29 publications

(35 citation statements)

References 32 publications

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“…In terms of orthodontic treatment, each study examined exclusively fixed appliance therapy except for two studies. One of the studies was an orthognathic surgery study while the other compared root resorption in an untreated group …”

Section: Resultsmentioning

confidence: 99%

“…One study used protease inhibitors . One study used amylase/immunoglobulin/albumin depletion protocol . Removal of debris (through centrifugation) and fast freezing are known and acceptable processes to preserve protein biomarkers .…”

Section: Discussionmentioning

confidence: 99%

“…Concentrating samples as shown by Ellias et al seem to help pick up more inflammatory biomarkers. Removing highly abundant proteins in saliva such as amylase, immunoglobulins and albumin, for instance depletion assay, had shown a promising effect . Using multiplex system to detect salivary cytokines may also be an option .…”

Section: Discussionmentioning

confidence: 99%

“…Finally, three studies examined other factors that may be unique to that population including orthodontically induced inflammatory root resorption (OIIRR), obesity and orthognathic surgery. For example, Kaczor‐Urbanowicz et al identified biomarkers that may be related more to the bone healing from surgery such as vitamin D binding precursor increased, fibrinogen alpha chain precursor and wound healing such as complement C3. These non‐orthodontic factors may have influenced the biomarkers and masked the effect of orthodontic treatment.…”

Section: Discussionmentioning

confidence: 99%

“…One of the studies was an orthognathic surgery study 15 while the other compared root resorption in an untreated group. 16 Whole saliva collection protocol was used in all studies. However, Table 2).…”

Section: Re Sultsmentioning

confidence: 99%

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Salivary protein biomarkers associated with orthodontic tooth movement: A systematic review

Allen

Edelmann

Abdulmajeed

et al. 2019

Orthod Craniofacial Res

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Structured Abstract Objective Saliva can provide a non‐invasive approach to indicate changes in the oral and systemic conditions. Salivary proteomics allows the discovery of new protein biomarkers associated with certain conditions. The effectiveness and physiological effects of orthodontic tooth movement in theory can be measured using salivary protein biomarkers. Setting and Sample Population This study applied a systematic review to analyse current literature to define and summarize salivary biomarkers associated with orthodontic tooth movement identified by mass spectrometry proteomics and other protein detection techniques. Materials and Methods Peer‐reviewed articles published through the 15th of November 2018 via the PubMed, EMBASE, Web of Science and Dentistry & Oral Sciences databases were reviewed. Only studies analysing protein biomarkers in saliva samples collected from human subjects associated with orthodontic treatments were included. Results Out of 482 articles, 7 studies were selected. Sample size ranged from 3 to 72 subjects. Minor variations of unstimulated whole saliva sample collection protocol were noted. Mass spectrometry proteomics and ELISA represented the majority of biomarker discovery and targeting, respectively. Twenty biomarkers were identified or chosen as target biomarkers. Conclusion Salivary proteins may be used to indicate effectiveness of orthodontic treatment and orthognathic treatment as well as adverse treatment consequence, such as root resorption.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%