Identification of Salt-induced Changes in Leaf and Root Proteomes of the Wild Tomato, Solanum chilense

Zhou, Suping; Sauvé, Rémy; Zong, Liu; Reddy, Sasikiran; Bhatti, Sarabjit; Hucko, Simon; Fish, Tara; Thannhauser, Theodore W.

doi:10.21273/jashs.136.4.288

Cited by 43 publications

(29 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The inhibition of growth observed in many plants under salinity conditions is usually associated with several physiological disturbances, including ion toxicity, osmotic stress and generation of reactive oxygen species (ROS; Parida and Das, 2005). Zhou et al (2011) recently conducted a thorough proteomic analysis in S. chilense and demonstrated that salt-induced proteins are mainly associated with selective ion transport, carbohydrate metabolism and antioxidant activities. It was also recently demonstrated that S. chilense displays a fascinating ability to cope with salt-induced oxidative stress at the seedling stage (Martínez et al, submitted).…”

Section: Solanum Lycopersicum Solanum Chilensementioning

confidence: 99%

EFFECTS OF SALINE WATER ON WATER STATUS, YIELD AND FRUIT QUALITY OF WILD (SOLANUM CHILENSE) AND DOMESTICATED (SOLANUM LYCOPERSICUM VAR. CERASIFORME) TOMATOES

et al. 2012

View full text Add to dashboard Cite

SUMMARYFarmers around the world are concerned about the effects of human-induced salinity on crop yield and quality. Therefore, researchers are actively testing wild relatives of cultivated plants to identify candidates to improve crop performance under salt stress. A study was conducted to understand the effects of salt stress (Sodium chloride, NaCl) on cultivated tomato species (Solanum lycopersicum var. cerasiforme L.) and a wild tomato relative (Solanum chilense Dun.) from the Northern part of Chile. Plants were cultivated hydroponically under controlled environmental conditions for 112 days with nutrient solution containing 0 mM (3 dS m−1), 40 mM (6 dS m−1) and 80-mM (9 dS m−1) NaCl. Salt stress reduced the shoot biomass in S. lycopersicum but not in S. chilense. Both species were able to maintain the leaf water content; however, the cultivated S. lycopersicum showed osmotic adjustment, while S. chilense did not. Salt stress reduced the total fruit yield in S. lycopersicum based on a decrease in the mean fruit weight, but it had no impact on the number of fruits per plant. In contrast, salt stress had no significant impact on the fruit yield in S. chilense. Salt stress increased the total soluble solids content in S. lycopersicum and the titratable acidity in S. chilense. It was concluded that S. chilense displays a contrasting behaviour in response to prolonged exposure to moderate salinity compared with S. lycopersicum, and that this related species could be an interesting plant for breeding purposes.

show abstract

Section: Solanum Lycopersicum Solanum Chilensementioning

confidence: 99%

EFFECTS OF SALINE WATER ON WATER STATUS, YIELD AND FRUIT QUALITY OF WILD (SOLANUM CHILENSE) AND DOMESTICATED (SOLANUM LYCOPERSICUM VAR. CERASIFORME) TOMATOES

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Therefore, comparative proteomic approaches are powerful and promising tools for investigating plant stress responses. Several studies focused on proteomic changes in different plant organs, including roots and leaves, in response to salt stress in soybean ( Glycine max ), [ 14 ] and in wild tomato ( Solanum chilense ) [ 15 ]. Proteomic changes in leaves of wild and modern wheat under drought stress were studied to determine the differentially expressed proteins; the results revealed the differences in leaf tissue protein levels between modern and wild wheat genotypes in response to drought [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Physiological and Comparative Proteomic Analysis Reveals Different Drought Responses in Roots and Leaves of Drought-Tolerant Wild Wheat (Triticum boeoticum)

Liu

et al. 2015

PLoS ONE

View full text Add to dashboard Cite

To determine the proteomic-level responses of drought tolerant wild wheat (Triticum boeoticum), physiological and comparative proteomic analyses were conducted using the roots and the leaves of control and short term drought-stressed plants. Drought stress was imposed by transferring hydroponically grown seedlings at the 3-leaf stage into 1/2 Hoagland solution containing 20% PEG-6000 for 48 h. Root and leaf samples were separately collected at 0 (control), 24, and 48 h of drought treatment for analysis. Physiological analysis indicated that abscisic acid (ABA) level was greatly increased in the drought-treated plants, but the increase was greater and more rapid in the leaves than in the roots. The net photosynthetic rate of the wild wheat leaves was significantly decreased under short-term drought stress. The deleterious effects of drought on the studied traits mainly targeted photosynthesis. Comparative proteomic analysis identified 98 and 85 differently changed protein spots (DEPs) (corresponding to 87 and 80 unique proteins, respectively) in the leaves and the roots, respectively, with only 6 mutual unique proteins in the both organs. An impressive 86% of the DEPs were implicated in detoxification and defense, carbon metabolism, amino acid and nitrogen metabolism, proteins metabolism, chaperones, transcription and translation, photosynthesis, nucleotide metabolism, and signal transduction. Further analysis revealed some mutual and tissue-specific responses to short-term drought in the leaves and the roots. The differences of drought-response between the roots and the leaves mainly included that signal sensing and transduction-associated proteins were greatly up-regulated in the roots. Photosynthesis and carbon fixation ability were decreased in the leaves. Glycolysis was down-regulated but PPP pathway enhanced in the roots, resulting in occurrence of complex changes in energy metabolism and establishment of a new homeostasis. Protein metabolism was down-regulated in the roots, but enhanced in the leaves. These results will contribute to the existing knowledge on the complexity of root and leaf protein changes that occur in response to drought, and also provide a framework for further functional studies on the identified proteins.

show abstract

“…Rick Tomato Genetics Resource Center, University of California, Davis. Germinated seeds were grown to the two-leaf stage in seedling cubes (Smithers-Oasis, Kent, OH) and then transferred into net pots (3.81 cm wide) filled with hydroteon clay balls (Hydrofarm, Seattle, WA) and continued to grow in half-strength Hoagland's nutrient solution (Hoagland and Arnon, 1950) to the four-leaf stage (Zhou et al, 2011). The greenhouse conditions were set at 25°C from 0500 to 2000 HR and 21°C during the remaining dark period with no supplemental lighting.…”

Section: Methodsmentioning

confidence: 99%

“…Tissues were ground into a fine powder under liquid nitrogen and then re-suspended in acetone supplemented with 10% trichloroacetic acid and 1% dithiothreitol (Sigma, St. Louis, MO). After incubation at -20°C overnight followed by centrifugation at 12,000 g n for 10 min at 4°C, protein pellets were collected and washed four times with pre-chilled acetone and finally evaporated to nearcomplete dryness (Zhou et al, 2009(Zhou et al, , 2011. After adding a dissolution buffer (1:10, w/v) consisting of 50 mM triethylammonium bicarbonate (TEAB) and 500 mM urea (Sigma), protein powder was incubated on ice for 10 min followed by centrifugation at 10,000 g n for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Differential Root Proteome Expression in Tomato Genotypes with Contrasting Drought Tolerance Exposed to Dehydration

Zhou¹,

Palmer²,

Zhou³

et al. 2013

J. Amer. Soc. Hort. Sci.

Self Cite

View full text Add to dashboard Cite

A comparative proteomics study using isobaric tags for relative and absolute quantitation (iTRAQ) was performed on a mesophytic tomato (Solanum lycopersicum) cultivar and a dehydration-resistant wild species (Solanum chilense) to identify proteins that play key roles in tolerance to water deficit stress. In tomato ‘Walter’ LA3465, 130 proteins were identified, of which 104 (80%) were repressed and 26 (20%) were induced. In S. chilense LA1958, a total of 170 proteins were identified with 106 (62%) repressed and 64 (38%) induced. According to their putative molecular functions, the differentially expressed proteins belong to the following subgroups: stress proteins, gene expression, nascent protein processing, protein folding, protein degradation, carbohydrate metabolism, amino acid and nucleotide metabolism, lipid metabolism, signal transduction, and cell cycle regulation. Based on changes in protein abundance induced by the dehydration treatment, cellular metabolic activities and protein biosynthesis were suppressed by the stress. In S. chilense, dehydration treatment led to elevated accumulation of proteins involved in post-transcriptional gene regulation and fidelity in protein translation including prefoldin, which promotes protein folding without the use of adenosine-5′-triphosphate (ATP), several hydrophilic proteins, and calmodulin in the calcium signal transduction pathway. Those protein changes were not found in the susceptible tomato, ‘Walter’. Within each functional protein group, proteins showing opposite changes (dehydration induced vs. repressed) in the two species were identified and roles of those proteins in conferring tolerance to water deficit stress are discussed. Information provided in this report will be useful for selection of proteins or genes in analyzing or improving dehydration tolerance in tomato cultivars.

show abstract

Identification of Salt-induced Changes in Leaf and Root Proteomes of the Wild Tomato, Solanum chilense

Cited by 43 publications

References 62 publications

EFFECTS OF SALINE WATER ON WATER STATUS, YIELD AND FRUIT QUALITY OF WILD (SOLANUM CHILENSE) AND DOMESTICATED (SOLANUM LYCOPERSICUM VAR. CERASIFORME) TOMATOES

EFFECTS OF SALINE WATER ON WATER STATUS, YIELD AND FRUIT QUALITY OF WILD (SOLANUM CHILENSE) AND DOMESTICATED (SOLANUM LYCOPERSICUM VAR. CERASIFORME) TOMATOES

Physiological and Comparative Proteomic Analysis Reveals Different Drought Responses in Roots and Leaves of Drought-Tolerant Wild Wheat (Triticum boeoticum)

Differential Root Proteome Expression in Tomato Genotypes with Contrasting Drought Tolerance Exposed to Dehydration

Contact Info

Product

Resources

About