Identification of salt tolerance QTL in a wheat RIL mapping population using destructive and non-destructive phenotyping

Asif, Muhammad; Garcia, Melissa; Tilbrook, Joanne; Brien, Chris; Dowling, Kate; Berger, Bettina; Schilling, Rhiannon K.; Short, Laura; Trittermann, Christine; Gilliham, Matthew; Fleury, Delphine; Roy, Stuart J.; Pearson, Allison S.

doi:10.1071/fp20167

Cited by 29 publications

(19 citation statements)

References 80 publications

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“…There have been numerous reports detailing the balance of ions (Na + , K + , and Ca 2+ , among others) related to plant salt tolerance [ 15 , 29 , 30 ]. In bread wheat, shoot ion-independent tolerance is considered an important trait for salt tolerance, favoring tissue expansion and tillering of plants before the shoots are affected by salt toxicity [ 31 , 32 ]. In general, the Na + content in roots and shoots of a non-halophyte will increase to varying degrees under salt stress, while the contents of K + and Ca 2+ will decrease to varying degrees [ 33 , 34 , 35 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

Root Na+ Content Negatively Correlated to Salt Tolerance Determines the Salt Tolerance of Brassica napus L. Inbred Seedlings

Wang

Han

Qiao

et al. 2022

Plants

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Soil salinization is a major environmental stressor that reduces the growth and yield of crops. Maintaining the balance of ions under salinity is vital for plant salt tolerance; however, little is known about the correlation between the salt tolerance of crops and the ion contents of their roots and shoots. Here, we investigated the poorly understood salt-tolerance mechanisms, particularly regarding ion contents (particularly Na+), in Brassica napus subsp. napus L., an agriculturally important species. Twenty B. napus inbred lines were randomly chosen from five salt-tolerance categories and treated with increasing concentrations of NaCl (0–200 mmol) for this work. We found that the root Na+ content is the most correlated limiting factor for the salt tolerance of B. napus; the higher the salt tolerance, the lower the root Na+ content. Correspondingly, the Ca2+/Na+ and K+/Na+ ratios of the roots were highly correlated with B. napus salt tolerance, indicating that the selective absorption ability of these ions by the roots and their translocation to the shoots play a pivotal role in this trait. These data provide a foundation for the further study of the molecular mechanisms underlying salt tolerance and for breeding salt-tolerant B. napus cultivars.

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Section: Introductionmentioning

confidence: 99%

Root Na+ Content Negatively Correlated to Salt Tolerance Determines the Salt Tolerance of Brassica napus L. Inbred Seedlings

Wang

Han

Qiao

et al. 2022

Plants

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“…Co‐location of ionic concentration (Na + , K + and Cl − ) QTL has also been observed in barley (Nguyen et al., 2013), rice (Ammar et al., 2009; Pandit et al., 2010) and wheat (Asif et al., 2020; Genc et al., 2014). Clustering of these QTL indicates that one or more genes/transporters are controlling the transport of these three ions.…”

Section: Discussionmentioning

confidence: 90%

An approach to detecting quantitative trait loci and candidate genes associated with salinity tolerance in faba bean (Vicia faba)

Asif

Paull

2021

Plant Breeding

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Faba bean is one of the most important high protein cool‐season grain legumes, but it is sensitive to salinity stress. Improved salt tolerance of faba bean is needed to increase its yield and area of production. In this study, a recombinant inbred line (RIL) population, derived from the bi‐parental cross of 'Icarus' × 'Ascot', was phenotyped under control and saline treatments. Quantitative trait locus (QTL) analysis of this population detected multiple QTL for leaf ionic concentration (Na+, K+ and Cl−) under both treatments. Of these, seven QTL were detected under salt treatment, including three for leaf K+:Na+ (qK:Na‐S‐1, qK:Na‐S‐2 and qK:Na‐S‐3) and one each for Na+ accumulation (qNa‐S), K+ accumulation (qK‐S), Cl− accumulation (qCl‐S) and Na+:K+ (qNa:K‐S). An important region was identified on linkage group I.A/III/V, which contained three co‐located QTL (qNa‐S, qCl‐S and qK:Na‐S‐3) for leaf Na+, Cl− and K+:Na+ accumulation. Potential candidate genes within these QTL intervals were shortlisted using the Medicago truncatula genome. These findings are expected to facilitate the breeding of new salt tolerant faba bean cultivars.

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“…(2021) ; Olatunji and Afalayan (2019) ; Rawandoozi et al. (2021) Asif et al. (2020) Association mapping SSR; SSR; SNP Maize ( Zea mays ); Chickpea; Maize ( Zea mays ) Jha et al.…”

Section: The Concept Of Molecular or Dna Markersmentioning

confidence: 99%

Basic concepts and methodologies of DNA marker systems in plant molecular breeding

Amiteye

2021

Heliyon

126

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The concepts, methodologies and applications of some of the major molecular or DNA markers commonly used in plant science have been presented. The general principles of molecular marker techniques have been elucidated with detailed explanation of some notable basic concepts associated with marker applications: marker polymorphism, dominant or co-dominant mode of inheritance, agronomic trait-marker linkage, genetic mutations and variation. The molecular marker methods that have been extensively reviewed are RFLP, RAPD, SCAR, AFLP, SSR, CpSSR, ISSR, RAMP, SAMPL, SRAP, SSCP, CAPS, SNP, DArT, EST, and STS. In addition, the practicality of the retrotransposon-based marker methods, IRAP, REMAP, RBIP, and IPBS, have been discussed. Moreover, some salient characteristics of DNA markers have been compared and the various marker systems classified as PCR-or non-PCR-based, dominantly or co-dominantly inherited, locus specific or non-specific as well as at the levels of marker polymorphism and efficiency of marker reproducibility. Furthermore, the principles and methods of the following DNA markers have been highlighted: Penta-primer amplification refractory mutation system (PARMS), Conserved DNA-Derived Polymorphism (CDDP), P450-based analogue (PBA) markers, Tubulin-Based Polymorphism (TBP), Inter-SINE amplified polymorphism (ISAP), Sequence specific amplified polymorphism (S-SAP), Intron length polymorphisms (ILPs), Inter small RNA polymorphism (iSNAP), Direct amplification of length polymorphisms (DALP), Promoter anchored amplified polymorphism (PAAP), Target region amplification polymorphism (TRAP), Conserved region amplification polymorphism (CoRAP), Start Codon Targeted (SCoT) Polymorphism, and Directed Amplification of Minisatellite DNA (DAMD). Some molecular marker applications that have been recently employed to achieve various objectives in plant research have also been outlined. This review will serve as a useful reference resource for plant breeders and other scientists, as well as technicians and students who require basic know-how in the use of molecular or DNA marker technologies.

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Identification of salt tolerance QTL in a wheat RIL mapping population using destructive and non-destructive phenotyping

Cited by 29 publications

References 80 publications

Root Na+ Content Negatively Correlated to Salt Tolerance Determines the Salt Tolerance of Brassica napus L. Inbred Seedlings

Root Na+ Content Negatively Correlated to Salt Tolerance Determines the Salt Tolerance of Brassica napus L. Inbred Seedlings

An approach to detecting quantitative trait loci and candidate genes associated with salinity tolerance in faba bean (Vicia faba)

Basic concepts and methodologies of DNA marker systems in plant molecular breeding

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